目的 探讨非SMC凝集素Ⅰ复合亚基D2(NCAPD2)在口腔鳞状细胞癌(OSCC)中的表达及生物学功能.方法 首先应用数据库分析NCAPD2在头颈部鳞状细胞癌(head and neck squamous cell carcinoma,HNSCC)中的表达,然后采用免疫组化法在OSCC标本中进行验证.应用高内涵筛选(high?content screening,HCS)、流式细胞仪及蛋白质印迹法(Western Blot)检测OSCC细胞的增殖、凋亡及凋亡相关蛋白的表达.结果 NCAPD2在HNSCC中高表达,且与HNSCC临床分期、病理分级和淋巴结转移有关.免疫组化显示OSCC组织中的NCAPD2表达显著高于健康口腔组织(P<0.001),且NCAPD2表达与OSCC病理分级之间存在显著相关性.敲减NCAPD2能够抑制OSCC细胞的增殖促进凋亡.此外,BCL?2,p21表达下调,BAX,p53表达上调.结论 NCAPD2有望成为OSCC治疗的一个靶点.

<span id="ChDivSummary" name="ChDivSummary" class="abstract-text">肠道菌群在肥胖的发生发展过程中起着重要的作用。肠道微生物的种类和相互比例与肥胖密切相关,肠道菌群通过宿主与饮食交互作用调节能量代谢,肠道菌群的改变也可导致过多毒素产生进而诱发炎症反应和促进脂肪代谢综合征。本文就近年来有关肠道微生物与肥胖相关研究进展进行综述。</span>

目的了解武汉市售蔬菜、水果中农药残留现状及趋势,为政府有关部门制定针对性强的、有效的农药监管防治对策和为消费者自己的预防保护提供参考依据。方法于2009—2011年每年7月从武汉市某市场采集样品,利用安鑫宝PR-3新型果蔬农药残留速测仪（以及配套的试纸）进行农药残留检测。结果 2009—2011年检测水果蔬菜共191种,总阳性率为38.22%。阳性率呈逐年递增趋势。水果类与蔬菜类农药检出率差异有统计学意义（χ2=5.68,P＜0.05）。结论武汉市农贸市场销售的蔬果中农药残留情况较为严重,建议政府有关部门从农药生产、销售源头加强管理,开展农药残留危害及其防范的宣传教育。消费者从挑选、暴晒、去皮等途径加强自我保护,防治农药危害身体健康。

Objective To investigate the effects of ginkgo biloba extract(GBE) on expressions of IL-1β,IL-6, and TNF-α in the pancreas and brain tissues of rats with severe acute pancreatitis(SAP),and further to explore the pathogenesis of SAP and the efficacy of GBE on brain injury.Methods Fifty-four Winstar rats were randomly divided into normal control group,model group,and treatment group,with 18 rats for each group.For rats in the normal control group,only conversion of pancreas was performed by abdomen opening,followed by wound closure immediately.For rats in the model group and treatment group,5% sodium taurocholate hydrate were injected under pancreatic capsule to establish SAP model,and then GBE and normal saline were infected into intra-abdomen repeatedly every 8 hours,respectively.At 6 h,12 h,and 24 h after the model establishment,experimental samples were extracted and serum amylase was detected.Pathogenic scoring for pancreas tissues was performed under light microscopy,and immunohistochemistry method was employed to detect the expression levels of IL-1β,IL-6,and TNF-α in pancreas and brain tissues.Results For the treatment group,both serum amylase and pancreas scoring were significantly lower than those of the model group(P0.01).At 24 h after model establishment,the expressions of IL-1β,IL-6,and TNF-α of pancreas tissues in model group were significantly higher than those at 6 h and 12 h(P0.05 or P0.01),but no significant differences were observed in treatment group(P0.05).The expressions of IL-1β,IL-6,and TNF-α of brain tissues in model group were significantly higher than those at 6 h and 12 h(P0.05 or P0.01),but in treatment group decreased(P0.05 or P0.01).The expressions of IL-1β,IL-6,and TNF-α in the treatment group were significantly lower than those of the model group at same time(P0.01).Conclusions During SAP,the expressions of IL-1β,IL-6 and TNF-α in pancreas and brain tissues increased obviously.GBE showed suppressing and scavenging effects on IL-1β,IL-6 and TNF-α in pancreas and brain tissues.

对现在在职护理工作人员的工作状态和胜任能力的了解和分析,发现护理中存在的缺陷,通过学习教育寻找解决方法,提升工作能力和培养团队管理能力。

Objective To summarize the clinical experience(154cases) of the mesh plug repair for groin hernias.Methods 154 patient s were treated by mesh plug hernia repair with mading china.,from January 2008 to January 2010.The oper -ative procedure,surgical indicati- on,ability recove -ry,postoperative pain,complications,hospitalized period and recurrence rate were studied.Results The mesh plug hernia repair had a greater advantage than that of t raditional hernia repair procedure,such as easier surgical performance,a wider surgical indication,less postoperative pain,quicker recovery,shorter period of hospita -lization,fewer comp -lications and lower recurrence.Conclusions It is currently the most popular choice for a convenient hernia repair pro -cedure.It has been an out standing method in t reating groin hernia.

Objective To explore correlating factors of surgical treatment and prognosis of colorectal signet-ring cell carcinoma.Methods Clinical pathological data and follow-ups were analyzed retrospectively in 62 patients with colorectal signet-ring cell carcinoma hospitalized during January 1988~December 2006.Survival genetic data were evaluated by the Kaplan-Meier methods.Results All the 62 patients underwent rectum surgeries(33 underwent radical resection;19 underwent colostomy or exploratory surgery and 10 underwent palliative resection,diagnosed as signet-ring cell carcinoma,37 of whom underwent postoperatived adjunctive chemotherapy and 55 patients in the group were followed up).Median survival time was 28 months and the meta-survival time of radical incision,palliation incision and non surgical incision were 47,19,10 months respectively.The difference was of statistical significance(P<0.05).Regression analysis revealed that bowel obstruction,vessel tumor embolism,tumor infiltration,lymph node metastasis,operative modus and postoperative adjunctive therapy were independent prognostic factors.Conclusion Prognosis of colorectal signet-ring cell carcinoma tends to be unsatisfactory.Early diagnosis with radical resection and postoperative adjunctive therapy can improve prognosis.

微量元素在人体中起着多种重要的生理功能,许多肝脏疾病的发生,发展与微量元素在机体的变化及两者间的关系,逐渐受到人们的关注,而肝脏作为有许多重要生理功能的人体最大腺体,对微量元素的转运和分布起关键作用.

[Objective]To summarize the experience of diagnosis and surgical treatment of acute gallstone pancreatitis,so as to improve the diagnosis and treatment levels.[Methods]The clinical data of 86 gallstone pancreatitis cases in Inner Mongolia Medical College Affiliated People's Hospital of recent 8 years were retrospectively analyzed.[Results]Emergency surgery was performed on 17 cases of 86 gallstone pancreatitis patients,including cholecystectomy,choledochotomy,T-tube drainage,and pancreatitic capsule incision based on condition of pancreatitis,removaling of pancreatic necrosis lesions,and small omental drainage cavity lavage.For other 69 cases,firstly the conservative treatment was taken firstly,then operations were performed when the inflammation was subsided.[Conclusion]A clear diagnosis of gallstone pancreatitis should be made as soon as possible,the choice of emergency surgery or conservative treatment with following appropriate surgical approach at appropriate time after the subsided inflammation is based on whether there is obvious obstruction of biliary tract or not.The appropriate treatment can control disease development and reduce complications and improve cure rates of the disease.

作者总结近年收治的184例老年胆系患者外科治疗情况,就其临床特点,手术时机的选择,手术方式的确定及并发症防治等方面进行了讨论。

<正>为探讨慢性结石性胆囊炎与肝脏损害程度的关系,对3年内因慢性结石性胆囊炎行择期胆囊切除术中60例<60岁患者术中取肝组织进行活检,现将结果分析报道如下。1临床资料1.1病例选择标准①60岁以下;②非急性发

总结了胆管空肠Roux—Y吻合术后再次手术23例资料,占同期此类手术7.5%。经手术证实再手术原因为肝胆管结石复发,前次手术病灶清除不彻底,吻合操作方法不当等。认为行胆管空肠Roux—Y吻合术必须要做到"取尽结石,清除病灶,纠正狭窄,通畅引流"。并对有关的具体问题进行了讨论。

[Objective] To explore the surgical principle and method for elderly left half colon cancer obstruction.[Methods] Surgical treatment results of 38 cases with colon cancer were retrospectively analyzed.[Results] All patients were conducted one-stage resection and anastomosis with intraoperative intestinal decompression and sausage.[Conclusion] Elderly patients with more complications and serious disease must be given surgery as soon as possible.If patients' conditions are permitted,one-stage resection and anastomosis is the best surgical program..

Objective:To investigate the causes of the postoperative complication from the Rectal carcinoma,and find the way to avoid them.Methods:Retrospective analyisi of 96 patients(from June 1995 to May 2005)who suffered from the complication after the operation.Results:In all of the patients.There are nineteen cases of complications,they includdthe early postoperative inflammationary ileus,the inosculation meatus fistula,the fistula meatus narrow、 the abscess around the fistula meatus、the necrosis with the intestines of the fistula meatus、the ureter hurt、the celiac hernia、the lung infection、the incision infection,the incision split,the lower limb'sdeep vein thrombus and so on.Conclusion:Part of the reason about the complication is from the source of medicine.Active prevention,reasonable treatment and canonical operation is linchpin of decrease complication,increase curative effect.

目的:探讨应用羟基喜树碱(HCPT)膀胱内灌注,预防浅表性膀胱癌复发的疗效。方法:术后使用羟基喜树碱20mg+生理盐水30ml膀胱内灌注21次预防肿瘤复发。结果:随访1~2年,35例中复发4例,复发率为11.4%,且并发症和副作用小。结论:HCPT膀胱内灌注预防浅表性膀胱癌复发,疗效满意。

With a reference to the related articles,7cases of acute pancreatitis combined with pancreatic encephalopathy were reveiewed in order to discuss the causes,pathological physiology,clinical symptoms,diagnosis and treatment of pancreatic encephalopathy(PE).The main pathogenesis of PE was large quantity of enzymes,which were secreted by the pancreas,entering the blood and crossing the blood-brain barriers which resulted in cerebral hemorrhage,encephalomalacia,demyelination.That could give rise to neurasthenic syndrome.Pancreatic encephalopathy largely showed no signs of specificity and signs of localization which combined with intracranial hypertension and abnormal electroencephalogram.The author put forward the diagnosis criterion for refernce.The treatment principle was to perform an operation in early stage helping diminish inflammation which could block the subsequent damages associated with it.