目的:基于网络药理学和分子对接技术探讨"陈皮-山楂"药对治疗高脂血症(HLP)的作用机制.方法:通过使用TCMSP、ETCM和BATMAN-TCM数据库筛选陈皮、山楂的活性成分和作用靶点;通过Genecards、Disgenet和OMIM数据库获取HLP潜在作用靶点;通过Venny网站获取"陈皮-山楂"药对与疾病的交集靶点;使用Cytoscape软件构建活性"成分-疾病-交集靶点"网络图;使用String数据库和Cytoscape软件构建交集靶点蛋白质-蛋白质相互作用(PPI)网络;利用Metascape数据库对交集靶点进行基因本体(GO)生物功能分析及京都基因和基因组百科全书(KEGG)通路富集分析;最后利用AutoDock Vina软件对主要活性成分和关键靶点进行分子对接.结果:得到"陈皮-山楂"药对潜在活性成分71个、潜在作用靶点414个,HPL疾病靶点1056个,其中成分与疾病交集靶点共88个;GO生物过程作用于激素反应、细胞对激素刺激的反应等602个条目,GO细胞组分富集作用于转录调节复合物、囊泡腔等23个条目,GO分子功能富集作用于核受体活性、配体激活转录因子活性等47个条目;KEGG通路分析共得到127条通路,与脂肪细胞因子信号通路、PPAR信号通路和AMPK信号通路等有关;分子对接结果证实主要活性成分槲皮素、柚皮素与关键靶点PPARA、TP53结合能力良好.结论:"陈皮-山楂"药对可通过多成分、多靶点、多途径发挥对高脂血症的治疗作用.

Objective:To explore the relationship between high performance liquid chromatography (HPLC) fingerprint and toxicity of Gelsemium elegans fermented by Ganoderma lucidum ( Ganoderma lucidum- Gelsemium elegans) for different time. Methods:Gelsemium elegans was processed by biphasic solid-state fermentation with Ganoderma lucidum. A total of 10 samples of Ganoderma lucidum- Gelsemium elegans were collected after fermentation for 9, 11, 13, 15, 17, 19, 21, 23, 25, 27 days (sampling twice on day 27, number: S1-S10) and the fingerprints were determined by self-established HPLC. One hundred specific pathogen-free ICR mice were randomly divided into 10 groups (each group comprised 10 mice, half were male and half were female). The median lethal dose of Ganoderma lucidum- Gelsemium elegans collected after fermentation for 11 days in mice was used as the final concentration in toxicity test. S1-S10 Ganoderma lucidum- Gelsemium elegans solutions were prepared and given to 10 groups of mice respectively by gavage administration and the death of mice was observed. According to the calculation formula of grey correlation analysis, the correlation coefficients between the common peaks of S1-S10 Ganoderma lucidum- Gelsemium elegans in chromatographic fingerprint and their toxicity test results (death rate in mice) was calculated and the main components contributing to the toxicity of Ganoderma lucidum- Gelsemium elegans were analyzed. Results:A total of 17 common peaks were identified in the chromatographic fingerprint spectrum of S1-S10 Ganoderma lucidum- Gelsemium elegans. The mortalities in mice caused by S1-S10 of Ganoderma lucidum- Gelsemium elegans were 1.00, 1.00, 0.80, 0.70, 0.60, 0.60, 0.50, 0.40, 0, and 0, respectively. The grey correlation analysis showed that the correlation coefficients of common peak 7, 3, 6, 9, 1, 8, 17, and 12 to toxicity were 0.868, 0.838, 0.830, 0.828, 0.824, 0.820, 0.818, and 0.802, respectively. The chemical components represented by these 8 chromatographic peaks had more contribution to the toxicity of Ganoderma lucidum- Gelsemium elegans. Conclusions:With the extension of fermentation time, the toxicity of Ganoderma lucidum- Gelsemium elegans decreased gradually, and toxicity was the lowest at 27 days of fermentation. The toxicity of Gelsemium elegans after fermentation was the result of a join action from multiple components. The identification of the main toxicity components can provide a reference for the quality control of Ganoderma lucidum- Gelsemium elegans and the fermentation process optimization.

目的 比较台湾牛蒡根不同溶剂提取物的降血糖作用.方法 用水和95％乙醇提取台湾牛蒡根,即为水提物和醇提物.随机将56只SD雄性大鼠分为正常组8只和造模组48只,造模组采用高脂饲料+链脲佐菌素(STZ)建立大鼠糖尿病模型,正常组不做处理.空腹血糖≥11.1 mmol/L为造模成功,将造模成功的大鼠随机分为模型组,阳性组,醇提物高、低剂量组,水提物高、低剂量组,每组8只.水提物高剂量组和醇提物高剂量组分别灌胃水提物和醇提物600 mg/(kg·d),水提物低剂量组和醇提物低剂量组分别灌胃水提物和醇提物200 mg/(kg·d),阳性组灌胃二甲双胍150 mg/(kg·d),正常组、模型组灌胃生理盐水10 mL/(kg·d),每天上午9点灌胃给药1次,连续灌胃5周.每隔3d检测各组大鼠体质量、进食量、空腹血糖的变化,末次给药前1d检测各组大鼠糖耐量变化,绘制血糖曲线下面积(AUC).连续给药5周后,腹主动脉采血,检测血清TG、TC、HDL-C的变化.结果 与正常组比较,模型组体质量降低,进食量、空腹血糖升高,AUC增大(P<0.01);与模型组比较,醇提物高、低剂量组体质量、空腹血糖无变化,阳性组,水提物高、低剂量组体质量升高,进食量增加,空腹血糖降低,AUC减小(P均<0.01);与模型组比较,醇提物高、低剂量组大鼠血清TG、TC、HDL-C含量无变化,阳性组,水提物高、低剂量组大鼠血清TG、TC、HDL-C含量降低(P均<0.01).结论 台湾牛蒡根水提物可改善糖尿病大鼠血糖和血脂水平,以水提物高剂量效果最佳.

目的 建立体内、体外钩吻生物碱成分测定方法,并测定抵当汤减毒前后钩吻生物碱成分含量,探讨抵当汤减缓钩吻毒性的可能作用机制.方法 采用UPLC-QDa体外检测钩吻与抵当汤混合前后6种生物碱成分变化,采用UPLC-MS/MS检测抵当汤联合钩吻灌胃对小鼠血浆中钩吻生物碱成分含量的影响.结果 与抵当汤混合后,钩吻中钩吻素子、钩吻素甲、钩吻绿碱、胡蔓藤碱乙含量明显降低(P＜0.01);抵当汤十钩吻组小鼠血清钩吻素子、钩吻素甲、钩吻素己、钩吻绿碱、胡蔓藤碱乙、胡蔓藤碱丙含量均较钩吻组明显降低(P＜0.01).结论 降低钩吻素子、钩吻素甲、钩吻素己、钩吻绿碱及胡蔓藤碱乙含量可能是抵当汤减缓钩吻毒性的作用机制之一.

目的 采用一种新型低共熔溶剂超声辅助提取草珊瑚总黄酮,并对其提取工艺进行优化,旨在为草珊瑚资源的开发、利用提供参考.方法 通过单因素实验研究了低共熔溶剂的组成体系、组成比例、含水量、料液比(g/mL)、超声时间(min)、超声温度(℃)对草珊瑚总黄酮提取率的影响,在此基础上采用响应面法对草珊瑚总黄酮提取工艺进行优化及验证.结果 由氯化胆碱和乙二醇的摩尔比为1∶4(含水量为20％)制备的低共熔溶剂为草珊瑚总黄酮最佳提取溶剂,经响应面法优化后,在超声时间28 min、温度80℃和料液比1∶31 (g/mL)时对草珊瑚总黄酮的提取效果最好,平均提取率为12.80％.结论 超声辅助低共熔溶剂提取草珊瑚总黄酮具有较好的提取效果,该方法操作简单,绿色环保,在一定程度上可以促进草珊瑚的综合利用.

目的:在前期研究基础上,检测钩吻炮制前后的6种主要生物碱成分的变化情况,以期揭示钩吻炮制后减毒存效的内在机制.方法:采用HPLC同时测定钩吻素甲、胡蔓藤碱丙、钩吻素子、钩吻素己、钩吻绿碱、胡蔓藤碱乙6个成分的含量,色谱条件为流动相甲醇(A)-0.1％甲酸水溶液(B)梯度洗脱(0～ 10 min,22％A;10 ～ 20 min,22％ ～ 30％A;20 ～ 30 min,30％～ 40％ A),流速1 mL· min-1,检测波长254 nm,柱温30℃,进样量10μL.采用聚类分析和主成分分析法对钩吻炮制前后化学成分变化情况进行分析.结果:炮制前上述6种成分在12批生品中的平均质量分数分别为1.444,1.129,3.590,1.603,2.376,1.631 mg·g-1;炮制后六者的平均质量分数则依次为2.258,0.343,1.176,0.115,0.459,0.281 mg·g-1;毒性最强成分钩吻素己质量分数下降最为显著,下降率达92.83％,而毒性较小的钩吻素甲炮制后质量分数升高了56.37％,其他4个成分质量分数均有不同程度的降低.聚类分析结果表明钩吻炮制前后可明显分为2个类别,主成分分析表明钩吻炮制后第1主成分由钩吻素子变为钩吻素甲.结论:钩吻炮制后毒性成分钩吻素己被显著降解,胡蔓藤碱丙、钩吻素子、钩吻绿碱、胡蔓藤碱乙成分含量下降以及钩吻素甲的含量升高可能是钩吻炮制后减毒存效的内在机制之一.

目的 建立超高效液相色谱-质谱法(UPLC-MS)同时监测发酵钩吻中的7种有毒生物碱(常绿钩吻碱、钩吻素子、钩吻素甲、钩吻素乙、胡蔓藤碱丙、钩吻绿碱和胡蔓藤碱乙)随着发酵时间的含量变化.方法 用Waters CortecsC18(2.1 mm×100.0 mm,1.6 μm)色谱柱;以乙腈(A)-0.1％甲酸水(B)进行梯度洗脱(0～0.5 min,12％ ～ 12％A;0.5 ～8.0 min,12％～20％A;8.0～11.0min,20％ ～20％A;11.0 ～13.0 min,20％ ～40％A;13.0～17.0 min,40％～70％A;17～20.0 min,70％ ～ 70％A.流速0.25 mL· min-1,柱温45℃.在DAD和QDA光谱仪上进行检测和定量分析.结果 7种成分在各自浓度范围内呈现出良好的线性关系,均r≥0.999 5,平均回收率97.2％～ 101.1％,RSD为2.1％～3.4％(n=6).钩吻7种毒性生物含量分别为常绿钩吻碱65％、钩吻素子96％、钩吻素甲97％、钩吻素乙99％、胡蔓藤碱丙99％、钩吻绿碱95％和胡蔓藤碱乙99％,显示随着发酵的进行,含量显著降低.结论 该方法简便、准确和重复性良好.钩吻生物碱的含量在发酵过程逐渐降低.

目的 采用GC法同时测定不同干燥方式不同部位草珊瑚挥发油中α-蒎烯、莰烯、(-)-β-蒎烯、柠檬烯、芳樟醇、乙酸龙脑酯、β-榄香烯和β-石竹烯8种成分的含量.方法 色谱柱:HP-5毛细管色谱柱(30m×0.32mm×0.25 μm);检测器:FID;进样口温度:250℃;检测器温度:270℃;柱温:50℃为起始温度,保持2min,以5℃·min-1的速度升至130℃,保持5min;载气:氮气;流速:1 mL·min-;进样量:1μL;分流比:30∶1.结果 α-蒎烯、莰烯、(-)-β～蒎烯、柠檬烯、芳樟醇、乙酸龙脑酯、β-榄香烯和β-石竹烯色谱峰分离度良好;进样浓度分别在8.75～1750.00、5.62～1123.60、8.66～1732.80、4.37 ～873.60、1.96～391.20、4.98 ～995.60、8.54～1707.60、3.61 ～721.40μg· mL-1范围线性良好;平均加样回收率(n=6)分别为98.7％、97.2％、98.2％、98.3％、97.7％、97.5％、98.2％、97.8％,RSD分别为0.62％、1.21％、1.24％、1.30％、1.32％、1.27％、1.57％、0.77％.12批草珊瑚挥发油中α-蒎烯、莰烯、(-)-β-蒎烯、柠檬烯、芳樟醇、乙酸龙脑酯、β-榄香烯和β-石竹烯的含量(n=3)测定结果分别为0 ～225.50、0～167.06、0～250.34、0 ～55.66、0 ～77.91、0～201.98、2.35～294.49、0～14.82μg·g-1.结论 该方法准确可靠,适用于草珊瑚挥发油中8个活性成分的含量测定.

从中药学课程教学入手,从学情分析、教学设计、形成性评价三方面展开阐述,提出以中医药思维培养为根本,通过多种教学方法及评价手段,提高中药学类大学生对本专业的热爱程度,进而启发专业大学生创新创业意识,以促进培养适应中药类行业需求的专业人才.

目的 观察抵当汤对钩吻的解毒作用. 方法 通过预实验确认钩吻致死量后,采用传统水煎煮和乙醇提取方式的抵当汤与钩吻,通过不同给药方式的毒性试验检测小鼠死亡情况;给药后小鼠眼球后静脉丛取血和肺组织取材,观察各组血常规、乙酰胆碱酯酶变化以及小鼠肺组织HE染色情况. 结果 急性毒性预实验结果显示:钩吻的Dm剂量为1.5 g/kg.急性毒性实验结果显示:钩吻配伍抵当汤后,小鼠死亡率均明显下降,且死亡时间亦明显延长(P＜0.05).与空白组相比,钩吻组小鼠RBC、PLT、HGB、ACH与空白组比较呈显著性差异(P＜0.05),抵当汤钩吻混合液组小鼠RBC、PLT、HGB、ACH与钩吻组比较呈显著性差异(P＜0.05),而与空白组无差异.HE染色显示:钩吻抵当汤混合给药后小鼠肺组织细支气管、肺泡壁、肺间质中性粒细胞浸润伴充血减轻,肺泡壁毛细血管扩张不明显,肺泡腔渗出减少,肺组织局灶性实变消失. 结论 抵当汤能减缓钩吻毒性.

目的 建立HPLC同时测定不同采收期草珊瑚中新绿原酸、绿原酸、隐绿原酸、槲皮素-3-O-β-D-葡萄糖醛酸苷、异嗪皮啶、山柰酚-3-O-β-D-葡萄糖醛酸苷和迷迭香酸的含量.方法 采用北京慧德易SP ODS-AQ C18分析柱(4.6 mm×250 mm,5μm),流动相为乙腈(A)-0.2％磷酸水溶液(B),梯度洗脱(0～ 10 min,5％ A→15％ A;11～18 min,15％A→25％A;18～25min,25％ A;25 ～40 min,25％ A→40％ A),流速1.0mL· min-1,检测波长为344 nm,柱温35℃.结果 新绿原酸、绿原酸、隐绿原酸、槲皮素-3-O-β-D-葡萄糖醛酸苷、异嗪皮啶、山柰酚-3-O-β-D-葡萄糖醛酸苷和迷迭香酸分别在9.32 ～466.00、11.25 ～562.50、10.94 ～547.00、8.68 ～434.00、10.48 ～ 524.00、9.66～483.00和10.86～543.00 μg·mL-1内与峰面积呈现良好的线性关系(r =0.999 6 ～0.999 9),平均加样回收率(n=6)在99.0％～101.5％之间,RSD为0.85％ ～ 1.8％.主成分分析法综合评定得草珊瑚最佳采收月份为9、10、11月份.结论 该方法准确可靠,适用于草珊瑚中7个化学成分的含量测定,为草珊瑚的产地采收提供参考.

目的:研究不同干燥方式对草珊瑚有效成分含量的影响,为确定最佳干燥方法、提高草珊瑚质量提供依据.方法:采用阴干、晒干、不同温度烘干(40、50、60、70 ℃)、不同烘干时间(4、6 h)10种方法对样品进行干燥处理.采用HPLC法测定草珊瑚中异嗪皮啶、迷迭香酸、绿原酸、槲皮素-3-O-β-D-葡萄糖醛酸苷、山柰酚-3-O-β-D-葡萄糖醛酸苷5个有效成分的含量;运用TOPSIS综合分析法对不同干燥方式下的有效成分含量进行综合评价.结果:不同干燥方式对有效成分含量影响显著;综合评价结果表明,不同干燥方法下有效成分含量由高至低的排序为:50℃烘干4 h＞40℃烘干4 h＞50℃烘干6 h＞阴干＞40℃烘干6 h＞60℃烘干4 h＞60℃烘干6 h＞晒干＞70℃烘干4 h＞70℃烘干6h.结论:50℃烘干4h为10种干燥方式中最优的草珊瑚干燥方法,可为草珊瑚的产地加工提供借鉴.

Objective:To determine and compare contents of capiiliposides B and C in Lysimachia capillipes in different harvest time,so as to optimize the best harvest time.Methods:The Platisil C18 column (250 mm × 4.6 mm,5 μm) was used.Acetonitrile-0.3％ phosphoric acid (47 ∶ 53) was used as the mobile phase with isocratic elution at a flow rate of 1.0 mL· min-1.The tube temperature of ELSD was 90 ℃ and the flow rate of carrier gas was 1.0 L· min-1.Results:The calibration curves of capiiliposides B and C were in good linearity over 0.20-10.08 μg and 0.24-6.10 μg(r=0.999 7),respectively.The average recoveries were 96.1％ and 96.7％ with RSD of 0.99％ and 1.2％.Contents of two saponins in Lysimachia capillipes in different harvest time varied a lot and the higher contents were observed in samples harvested in July and November.Conclusion:The established method could determine capiiliposides B and C in Lysimachia capillipes simultaneously.The contents of the two components varied by different harvest time.The maximum total contents were observed in July and November.

目的 建立以秀丽隐杆线虫为模式生物的钩吻急性毒性快速筛选方法.方法 将线虫同步化到L4期,优化体系DMSO浓度、吐温-80用量、染毒时间,确定最优实验条件,建立以秀丽隐杆线虫为模式生物的钩吻急性毒性快速筛选方法.结果 以M9缓冲液为基本培养液,秀丽隐杆线虫在钩吻粗提物溶液中暴露72 h的LC50为39.722 mg·mL-1(95％置信限33.802～45.985 mg·mL-1).结论 该方法简便,快速,试验周期短,样品用量少,该方法可作为钩吻急性毒性研究中钩吻毒性变化的快速判断方法.

OBJECTIVE To optimize the formulation of self-microemulsion containing total saponins from Lysimachia capillipes by central composite design-response surface methodology.METHODS Based on the study of solubility,compatibility test and ternary phase diagram,central composite design-response surface methodology was adopted to optimize the best prescription with the emulsifying time,particle size,and Zeta pontential as indexes,physicochemical properties of this self-microemulsion were also determined.RESULTS Optimum formulation was 12.93％ of ethyl oleate,57.25％ of Kolliphor RH40,29.82％ of Transcutol.The average particle size,polydispersity index,Zeta pontential and drug loading of self-microemulsion containing total saponins from Lysimachia capillipes were 23.0 nm,0.160,－20.28 mV and 10.52 mg · g-1.CONCLUSION With good predictability,this methods can be used for the formulation optimization of self-microemulsion loaded total saponins from Lysimachia capillipes.

Objective:To develop an HPLC method for simultaneous determination of nine active components including astilbin,neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,caffeic acid,quercetin-3-O-β-D-glucuronide,isofraxidin,kaempferol-3-O-β3-D-glucuronide and rosmarinic acid in Sarcandra glabra.Methods:The Diamonsil C18 (250 mm × 4.6 mm,5 μm) column was used.Acetonitrile (A)-0.2％ phosphoric acid (B) was used as the mobile phase with gradient elution (0-20 min,2％ A → 15％ A;20-23 min,15％ A → 12.5％ A;23-42 min,12.5％ A;42-70 min,12.5％ A → 30％ A),at a flow rate of 1.0 mL· min-1.The detection wavelength was 290 nm for detecting astilbin and 344 nm for detecting neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,caffeic acid,quercetin-3-O-β-D-glucuronide,isofraxidin,kaempferol-3-O-β-D-glucuronide and rosmarinic acid.The column temperature was 35 ℃.Results:The calibration curves of astilbin,neochlorogenic acid,chlorogenic acid,cryptochlorogenie acid,caffeic acid,quercetin-3-O-β-D-glucuronide,isofraxidin,kaempferol-3-O-β-D-glucuronide and rosmarinic acid were in good linearity over 0.041-2.050,0.036 0-1.780,0.046-2.282,0.047-2.330,0.040-2.010,0.021-1.060,0.047-2.335,0.024-1.220,0.047-2.368 μg (r=0.999 8-1.000 0),respectively.The average recoveries (n=6)were in the ranges of 98.4％～101.7％ and RSD were in the ranges of 1.1％～2.7％.The contents (n=3) in 17 batches of samples of Sarcandra glabra were 0.29-1.09 mg· g-1 for astilbin,0.03-0.29 mg· g-1 for neochlorogenic acid,0.62-1.37 mg· g-1 for chlorogenic acid,0.38-1.32 mg· g-1 for cryptochlorogenic acid,0.06-0.42 mg· g-1 for caffeic acid,0.03-0.51 mg· g-1 for quercetin-3-O-β-D-glucuronide,0.17-0.68 mg· g-1 for isofraxidin,0.05-0.59 mg· g-1 for kaempferol-3-O-β-D-glucuronide,0.44-2.22 mg· g-1 for rosmarinic acid.Conclusion:The method is precise and reliable,and can be used to determine the contents of nine effective components in Sarcandra glabra.

目的 研究钩吻总碱对鸡胚绒毛尿囊膜(CAM)新生血管生成的影响. 方法 制备CAM模型,取50个鸡胚随机分为5组:空白对照组、阳性对照组及实验组(钩吻总碱高、中、低剂量组),于CAM上植入混合纤维素酯微孔滤膜药物载体,实验组加入不同浓度的钩吻总碱,空白对照组不加药品,阳性对照组加入PBS溶液,观察各组CAM新生血管生长的情况. 结果 钩吻总碱高、中剂量组及阳性对照组均有抑制CAM新生血管生成作用,而钩吻总碱低荆量组无抑制作用,与空白对照组作用相当. 结论 钩吻总碱具有抑制CAM新生血管生成的作用,并呈量效关系.

Objective:To study the toxicity attenuation and efficacy reservation of Pueraria Iobata processed by hot sand frying.Methods:Analgesic effect and acute toxicity were taken as evaluation index to select the preferable process by using oven heating method,with processing time and processing temperature as the independent variable in central composite design-response surface test.Results:The toxicity was significantly reduced,and certain analgesic effect was retained in Pueraria Iobata under 190 ～230 ℃ after 10 ～60 min of preparation.After comprehensive consideration,the optimized process was narrowed down to temperature of 200 ～220 ℃ and time 10 ～ 20 min after.The best process was finally confirmed at temperature of 210℃,processing time was 15min.Under this condition,the experimental results were in good agreement with the experimental predictions,which showed that the process parameters obtained by response surface method were reliable and had practical value.Conclusion:Hot sand frying can attenuate the toxicity and preserve analgesic effect of Pueraria Iobata.

目的:探讨钩吻生物碱类化学成分随湿热灭菌条件的变化规律.方法:采用高压蒸汽灭菌、低温间歇灭菌等不同条件,将钩吻藤茎粉末进行湿热灭菌,用HPLC法对灭菌前后的化学成分中的钩吻碱甲、钩吻素子、钩吻素己、胡蔓藤碱乙的含量进行监测.结果:60℃,70℃灭菌3次,钩吻生物碱成分含量未见明显变化,钩吻碱甲在各灭菌条件下较稳定.钩吻素己80C灭菌4次分别降低29.3％,28.2％,26.4％,32.8％,其余生物碱影响不大;90℃灭菌4次,钩吻素子分别降低8.5％,15.4％,15.4％,17.4％;钩吻素己分别降低32.8％,48.3％,55.2％,56.9％;胡蔓藤碱乙降低了1.3％,12.7％,12.7％,11.9％:121℃,21min高压灭菌对钩吻多种生物碱成分的破坏均较大,其中钩吻素己含量降低多达89％.结论:钩吻生物碱受湿热温度影响较大,其中钩吻素己最显著,80℃间歇灭菌4次是保证对钩吻灭菌较彻底且破坏性最低的灭菌方法.

目的 研究钧吻发酵解毒的机理. 方法 运用双向固体发酵原理与技术,将不同药用真菌接种到灭菌的钩吻药性基质上,在特定条件下进行发酵,得到药性菌质;用HPLC法对发酵前后菌质中的钩吻碱甲、钧吻素子、葫蔓藤碱甲、钩吻绿碱、葫蔓藤碱乙含量进行测定. 结果 相同发酵条件下,不同真菌对钩吻的发酵情况有所不同,灵芝、云芝、赤芝能够正常生长,其生物碱含量与钩吻生品比较呈下降趋势. 结论 双向固体发酵后菌丝体生长良好的菌质中,钩吻的液相色谱行为发生了质和量的变化.