以姜曲海猪精液为研究对象，分析冷冻-解冻后猪精子膜完整性及氧化应激水平，结果表明，新鲜精子经冷冻-解冻后，形态正常率、头部质膜完整率、尾部质膜完整率及SOD活性均显著降低，MDA含量显著升高。冷冻1组（未添加维生素C）与冷冻2组（添加维生素C）相比，前者精子形态正常率、头部质膜完整率、尾部质膜完整率及SOD活性分别为71．2％、57．2％、49．2％和85．3 IU/10-8，均显著低于后者，MDA含量则显著高于后者。冷冻-解冻造成了精子膜损伤，维生素C在一定程度上可有效提高精子的抗氧化能力，抵抗膜损伤。

校企合作体制机制创新已成为职业教育界关注的热门话题。本文在分析农业高职院校校企合作困境的基础上,以国家骨干高职院校江苏畜牧兽医职业技术学院理事会推动校企合作体制创新为例,探讨了农业高职院校校企合作的成功实践之路。

The talent cultivation mode is the core of higher occupation education connotation construction.In this paper,recognizing the connotation of talent cultivation model of higher vocational education,reflecting the national model of higher occupation education of agricultural professional personnel training mode reform,put forward " industry integration,linkage school " personnel training mode in Jiangsu Animal Husbandry & Veterinary College,and practice a variety of effective personnel training mode implementation forms at the professional level.

This essay aims to point out the achievements and deficiencies in putting project approach into effect by introduction of the practice of food sanitation inspection teaching innovation and analysis of the challenge of project approach.

The experiment was conducted to study the effects of phosphorus on the formation and resorption activity of muscovy duck's osteoclasts(OC)induced from bone marrow mesenchymal stem cells in vitro.Osteoclasts were isolated from long bones of 1 day old muscovy duck and cultured with different concentrations of NaH2PO4 and different concentrations of NaH2PO4 containing 10-8 mol/L 1,25-(OH)2D3,respectively.The tartrate-resistant acid phosphatase(TRAP)staining was carried out 6 h,12 h,18 h,24 h,30 h and 7 d after replacement of culture liquid with different concentrations of phosphorus,respectively,to count the number and observe the shape of TRAP positive OC,and acridine orange staining was carried out to observe the apoptosis of OC within 30 h,and lacunar resorption was detected on the 7th day through scanning electron microscopy.The result showed that adding phosphorus could inhibit the formation and bone resorption activity of OC induced from muscovy duck's bone marrow mesenchymal stem cells with 1,25-(OH)2D3 at the same time point,and the inhibition degree was direct proportional to the NaH2PO4 concentration from 3 mmol/L to 7 mmol/L.The number of OC co-cultured with phosphorus(≥3 mmol/L)was significantly smaller than that of control group(P0.01).The number of OC in 5 mmol/L group was significantly smaller than that in 3 mmol/L group(P0.01)and there was no significant difference between 5 mmol/L group and 7 mmol/L group.In addition,6,12,18,24 and 30 h after culture liquid replaced by adding different concentrations of phosphorus,the number of OC co-cultured with phosphorus(≥3 mmol/L)was significantly smaller than control group(P0.01),and the number of OC in group with the same concentration group was decreasing with the prolongation of time.In a word,phosphorus could inhibit the formation and resorption activity of OC and induce apoptosis of OC in vitro.

健康动物的骨骼处于不断重建的过程,骨重建(bone remodeling)是旧骨被吸收和新骨形成这一动态平衡过程[1].

中草药含有多种营养成分和生物活性物质,能增强机体的免疫功能,提高营养物质的消化利用率;副作用小,几乎不形成有害残留;资源丰富,可就地取材,且原料价格便宜,使用方便,对于畜牧业生产具有广阔的市场开发前景.

In order to develop a new type of Chinese herbal medicinal additive for preventing diseases and promoting growth in chicken, 4 compound prescriptions were prepared. The weight-gain of the chickens were compared in 3 batches of animal experiments. The results showed that prescription 4, composed of astragalus root, epimedium, hawthorn fruit and so on, could replenish Qi, improve digestion, regulate blood and promote Yang, which presented best efficacy and was superior to the antibiotic.

自从1992年秸秆养牛示范工程正式列入国家农业综合开发项目以来,目前已在全国范围内初步形成了中原、东北和华南三大肉牛带.1996年全国牛肉产量494.9万t,是项目开发前1991年的3.2倍,年递增率为26.61%.目前适度规模化的肉牛饲养已成为农业经济的支柱产业.规模化肉牛饲养管理技术是牛的科学选购、饲养管理、饲料加工等各项技术的综合运用.

应用饲养试验与氮平衡试验,研究了不同尿素水平的日粮对青山羊血清总蛋白(STP)、血清清蛋白(ALB)、血清球蛋白(GLB)、血清清球蛋白比值(A/G)、血清尿素氮(SUN)及血清谷丙转氨酶的含量变化的影响.试验采用4×4拉丁方试验设计,试验分4个阶段,在基础日粮一致的情况下,采用4、9、14、0(对照)g/kg·DM 4种不同的处理,试验结果表明:不同尿素水平极显著影响ALB、SUN浓度和GPT活性浓度(P＜0.01),对血清清球比(A/G)无显著差异(P＞0.05),尿素水平与STP、GLB浓度存在负相关;血清含氮生化指标STP、ALB、GLB、A/G、GPT、SUN随时间呈动态变化,出现高峰的时间比瘤胃氨浓度出现的时间平均推迟1～2h.进食尿素后,血液生化指标由平稳逐渐缓慢升高到高峰,随后又下降并趋于平衡.

2001年10月中旬,江苏某养鸽专业户饲养的300多对美国白羽王肉用鸽,部分鸽出现以神经症状和腹泻为主要特征的疫病,发病率为30%左右,死亡率为20%左右.经临床检查,病理剖检,初步诊断为鸽新城疫(ND).立即对全群鸽用新城疫疫苗紧急接种,迅速扑灭了疫情,同时从病死鸽体内分离到1株新城疫病毒(NDV).

赤羽病(akabane disease,AKA)又名阿卡斑病,是由赤羽病病毒(Akabane disease virus,AKV)引起的牛、绵羊和山羊的一种多型性传染病.AKA以流产、早产、死胎、畸形产为特征,能引起先天性关节弯曲和积水性无脑症.