<span id="ChDivSummary" name="ChDivSummary" class="abstract-text">目的观察白藜芦醇（resveratrol）对人肾癌786-O细胞迁移和侵袭能力影响,并探讨其作用机制。方法体外培养786-O细胞,分别以0（对照组）、10、20、40、80μmol/L白藜芦醇处理24、48、72 h,细胞计数试剂盒（CCK-8）检测786-O细胞活力。以0（对照组）、20、40、80μmol/L白藜芦醇处理786-O细胞24 h,Annexin V/PI双染法检测786-O细胞凋亡;克隆形成实验检测786-O细胞克隆形成能力;划痕损伤实验和Transwell小室体外侵袭实验分别检测786-O细胞迁移能力和侵袭能力;Western blot检测786-O细胞基质金属蛋白酶–2（MMP-2）、基质金属蛋白酶–9（MMP-9）表达。结果白藜芦醇以剂量和时间依赖性的方式抑制786-O细胞活力（P &lt;0.05）,其24、48、72 h IC50约为52.8、18.6、13.7μmol/L;与对照组[（4.30±0.55）%]比较,20、40、80μmol/L白藜芦醇组786-O细胞凋亡率[分别为（15.07±1.91）%、（19.70±2.25）%、（33.16±4.25）%]明显升高（P &lt;0.05）。与对照组比较,20、40、80μmol/L白藜芦醇组786-O细胞迁移率[分别为（77.68±8.42）%、（52.40±5.46）%、（39.73±4.35）%]明显降低（P &lt;0.05）,786-O细胞侵袭率[分别为（57.57±9.47）%、（31.87±5.21）%、（30.83±5.07）%]明显降低（P &lt;0.05）;与对照组比较,20、40、80μmol/L白藜芦醇组786-O细胞MMP-2、MMP-9表达明显降低（P &lt;0.05）。结论白藜芦醇可明显抑制786-O细胞迁移能力和侵袭能力,其机制可能与其抑制MMP-2和MMP-9表达有关。</span>

Objective To investigate the effects of quercetin on cell viability,apoptosis,autophagy,and phosphatidylinositol 3-kinase(PI3K)/protein kinase B(Akt)/mammalian target of rapamycin(mTOR)signaling pathway in human prostate cell carcinoma PC-3 cells.Methods PC-3 cells were cultured in vitro,and cell viability was detected by CCK-8.Apoptosis was detected by TUNEL staining.Autophagy vesicle was observed by acridine orange staining.Autophagosomes was observed by GFP-LC3 plasmid transfection analysis.Expressions of autophagy-related protein microtubule associated protein 1 light chain 3 fusion protein(LC3)and Beclin-1 and PI3K/Akt/mTOR signaling pathway protein were detected by Western blot analysis.Results Quercetin inhibited cell viability in a dose-time dependent manner and induced apoptosis.Quercetin increased the number of autophagy vesicles and autophagosomes in PC-3 cells.Quercetin increased the expressions of LC3-Ⅱ/LC3-Ⅰ and Beclin-1 in PC-3 cells and decreased the expression of phosphorylated-PI3K,phosphorylated-Akt and phosphorylated-mTOR.Conclusion Quercetin may induce autophagy by inactivating PI3K/Akt/mTOR signaling pathway in PC-3 cells.

目的 探讨血管紧张素转化酶抑制剂(ACEI类药物)在心力衰竭早期的临床干预效果,为临床用药提供参考.方法 收集符合诊断标准的心力衰竭早期患者104例,随机分为两组,对照组(49例)给予积极治疗原发病、控制危险因素等治疗,治疗组(55例)在上述治疗之上加用ACEI类药物:依那普利,干预6个月.观察干预3个月前后的心功能分级、氨基末端前体BNP(NT-proBNP)、心力衰竭超声指数变化,干预6个月心力衰竭超声指数变化及药物不良反应.结果 55例治疗组患者有50例(90.91％)维持在心功能I级,49例对照组患者有30例(61.22％)维持在心功能I级(P<0.05).两组干预前的NT-proBNP水平无显著差异(P<0.05);干预3个月,治疗组NT-proBNP水平较对照组显著下降(P<0.05).使用重复测量的方差分析结果表明,干预前、干预3个月及6个月心衰超声指数水平的两组间、不同时间及组别与时间的交互作用均有统计学意义(P<0.05),治疗组干预3个月、6个月的心力衰竭超声指数水平均较治疗前降低.治疗组药物不良反应发生率为9.09％,与对照组比较差异无统计学意义(P<0.05).结论 在心力衰竭的诊治过程中,应该重视心力衰竭的早期诊断,ACEI类药物早期干预能取得较好的治疗效果.

试验制备了十六烷基三甲基溴化铵稳定的十一烷醇乳液液滴,多巴胺通过氧化在乳液液滴表面形成聚多巴胺膜(pH 8.5,搅拌24 h).乙醇去除乳液模版十一烷醇后,即得到空心聚多巴胺胶囊.经过荧光显微镜,扫描电镜和紫外-可见分光光度计表征,制备的聚多巴胺微胶囊结构稳定.装载水溶性抗癌药物盐酸阿霉素后,中性环境下药物泄漏很少,而在酸性条件下则可加速其释放.

Objective:To analyze and compare the effect of the two classical formula with action of nourishing yin,Zuogui Pill and Liuwei Dihuang Pill,which have different compatibility characteristics,and to investigate relevant immune factors influenced by "Sanbu" (three medicinal with supplementing function),which is common to both formula,in subacute aging rats induced by D-galactose.Methods:The content of IL-1,IL-2,IL-8,TNF-α,and the expression of EGF in sub-acute aging rats were observed after the intervention with Zuogui Pill,Liuwei Dihuang Pill and "Sanbu".Results:After modeling,the content of IL-1,IL-8 and TNF-α were increased,but the content of IL-2 and the expression of EGF were decreased.Compared to the model group,the levels of IL-1 and TNF-α were degraded,and IL-2 level and EGF expression were raised obviously by the medications;IL-8 content was decreased obviously by Liuwei Dihuang Pill,however,there were no significant reducing effect in the other two groups.Conclusion:There are different inhibition effect on IL-1 and TNF-α,and up-regulation effect on IL-2 and EGF in sub-acute aging rats,when they are treated by Zuogui Pill,Liuwei Dihuang Pill,or "Sanbu",which may be related to the inhibition of the inflammatory factors and the adjustment of immune protective effect.There are different compatibility pattern in terms of reinforcing and reducing in the two formulas;comparatively,the effect of Liuwei Dihuang Pill is more obvious and stable,which shows appropriate compatibility playing an important role on the strength and stability of Chinese medicine formula.

老年人慢性胃炎的发病率明显高于年轻人〔1〕，并且老年慢性胃炎具有病势缠绵难愈，易于复发和癌变的特点。本文观察具有健脾行气作用的枳术散治疗老年慢性胃炎的疗效。

目的:观察咳尔康口服液对血管紧张素转化酶抑制剂(ACEI)诱发咳嗽的治疗效果.方法:选择符合纳入标准的ACEI诱发咳嗽患者148例随机分为治疗组(76例)和对照组(72例),治疗组口服咳尔康口服液(发明专利号ZL2009 10071953.6)10ml,每日3次;对照组给予安慰剂(外包装及药物颜色均与咳尔康口服液一致)10ml,每日3次口服;4周为1个疗程,观察两组患者治疗前后症状体征改善情况.结果:治疗组76例,显效55例,3个月随访过程中,22例无复发,16例于3个月内复发,9例于2个月内复发,8例于1个月内复发;18例有效,3例无效,总有效率96.05％.对照组72例,显效2例,有效19例,无效51例,总有效率29.17％,治疗前后主要症状积分变化有显著性差异(P＜0.01);与对照组相比,差异具有统计学意义(P＜0.01).结论:咳尔康口服液能有效缓解ACEI制剂诱发的咳嗽,疗效确切,方法简单安全,值得在临床上推广应用.

目的:观察具有健脾行气作用的枳术散配合针刺足三里对痞满证的治疗效果.方法:选择痞满证患者(年龄在40~70岁之间)59例,口服中药枳术散(白术和枳实按2:1比例为末)5 g,饭前以蜂蜜水送服,每日3次温服;恶食生冷者,以生姜水送服;伴胃脘疼痛者,可于疼痛发作时加次服药,每次5 g,服用次数不限;每日毫针刺双侧足三里穴1.5寸,平补平泻,留针30~40min,一个疗程(3周)后,观察患者治疗前后症状体征改善情况.结果:50例中,痊愈36例,显效9例,有效4例,无效1例,总有效率98%,治疗前后主要症状积分变化差异具有统计学意义,P<0.001.结论:口服枳术散配合针刺双侧足三里穴能有效缓解痞满证的各种症状,疗效确切,方法简单安全,值得在临床上推广应用.

目的:观察咳尔康口服液配合西药治疗慢性支气管炎急性发作期的临床疗效.方法:将 94 例慢性咳嗽患者随机分为治疗组47例和对照组47例.治疗组常规西药治疗的同时给予中药制剂咳尔康口服液,对照组给予常规西药治疗,疗程7d～10天.结果:治疗组的总有效率为95.74%,对照组的总有效率为87.23%,两组治疗后疗效积分对比有显著性差异,总体统计分析治疗组疗效优于对照组.结论:以培土生金法为主要配伍特征的咳尔康口服液对慢性支气管炎急性发作期具有较好的辅助抗炎和预防复发的效果,方法简单安全,值得在临床上推广应用.

目的:观察具有健脾行气作用的枳术散对老年人消化性溃疡的治疗效果.方法:选择消化性溃疡病老年患者(年龄60～80岁)30例,口服中药积术散(白术和枳实按2∶1比例为末)5g,饭前以蜂蜜水送服,每日3次温服.恶食生冷者,以生姜水送服；胃脘疼痛发作频繁者,可于疼痛发作时服药,每次5g,服用次数不限,1个疗程(3周)后,观察患者治疗前后胃镜检查及症状体征改善情况.结果:30例中,痊愈22例,显效5例,有效2例,无效1例,总有效率96.7％.治疗前后主要症状积分变化差异具有统计学意义(P＜0.01).结论:口服枳术散具有显著促进老年人消化性溃疡愈合和明显改善老年人消化性溃疡临床症状的作用.

Objective To analyze and compare the anti-aging mechanisms of the two invigorating yin formulas,Zuogui Pill and Liuweidihuang Pill,with different compatibilities of invigorating and reducing method and Sanbu group which were the main part of the two formulas.Methods The effect of Zuogui Pill,Liuweidihuang Pill and Sanbu group which are the main part of the two formulas on the restraining ability to free radical and expressions of P16 in subacute aging rats induced by D-galactose were detected.Results T-AOC,the quantity of CAT and the ability of restraining superoxide radical anion were cut down and H2O2 and the expression of P16 were up-regulated in subacute aging rats resulted by D-gal.The changes above-mentioned were reversed by Zuogui Pill,and so did by Liuweidihuang Pill except H2O2.T-AOC was raised and P16 proteinum expression was degraded by Sanbu group but CAT,superoxide radical anion and H2O2 were not changed markedly.Conclusion The anti-aging function of Sanbu group is weaker than that of the two formulas.It indicates that correct compatibility has important effect on complex prescription of Chinese medicine.

Objective To analyze and compare the function mechanisms preliminarily of two Tonify Yin Decoction with different supplementation and draining methods and three tonics Chinese medicine of Sanbu group.Methods It was observed that the effect of Zuogui Pill,Liuwei Dihuang Pill and Sanbu Decoction which were the main part of the two Decoction on ATPase and expressions of Bcl-2 and Bax which were agents related with apoptosis in subacute aging rats induced by D-galactose(D-gal).Results Consequence of ATPase in the liver and brain of rats were cut down and the expression of Bcl-2 and Bax in the hippocamp zone of rats' brain were stepped up.After the intervention of Zuogui Pill,Liuwei Dihuang Pill and Sanbu Decoction,the activity of Na+-K+-ATPase and Ca2+-Mg2+-ATPase and the expressions of Bcl-2 were raised in different degree and the expressions of Bax was descended.Conclusion The anti-aging function of Zuogui Pill,Liuwei Dihuang Pill and Sanbu Decoction may be concerned with that this formula can retain the stability of cell envelope and restrain apoptosis.There are no disparities of anti-aging functions in the two formulas with different compatibilities of invigorating and reducing method.It demonstrates that the Sanbu Decoction has the prevalent function in the two Decoction.

目的:探讨卡维地洛治疗老年人早期心力衰竭(以下称心衰)的疗效。方法:90例经超声心动图测定左心室射血分数(-LVEF)(45%,病情稳定无明显心衰症状和体征的老年患者随机分为治疗组(服卡维地洛),对照组(服安慰剂),经治疗1 a,观察两组病人治疗前后均需超声心动图测定心脏功能等变化。结果:治疗组在静息心率、血压、心脏功能等方面在治疗前后比较有显著差异(P(0.05),治疗效果显著。结论:卡维地洛治疗老年早期心衰可增加心脏射分数,明显改善心脏功能。

Objective To observe the clinic effects of treating senile viral myocarditis with Yixin decoction plus western medicine. Methods A retrospective analysis was performed on 98 cases with senile viral myocarditis from March 2005 to September 2007 in our hospital. The 98 cases were randomly recruited into a control group with 44 cases and a treatment group with 54 cases. The treatment group was treated with Yinxin decoction plus conventional western medicine and the control group was treated with conventional western medicine exclusively. Results The total effective rate in the treatment group and the control group was 98.15% and 75% respectively. The difference between the two groups were significant (P<0.05). Conclusion Yixin decoction plus western medicine has good effects in treating senile viral myocarditis.

目的:观察石牡钩莲汤降压及改善患者胰岛素抵抗的临床疗效.方法:以石牡钩莲汤治疗肝阳上亢型高血压病患者51例,与圣通平治疗的51例作对照.结果:治疗组降压有效率为90.2%,高于对照组60.8%(P＜0.05);治疗组TG、TC、LDL治疗后较治疗前均降低(P＜0.05),HDL升高(P＜0.01),而对照组治疗前后,血脂变化不明显(P＞0.05);治疗组可显著改善高血压患者的胰岛素抵抗(P＜0.01).结论:石牡钩莲汤不仅有明显的降压疗效,而且能改善高血压患者的胰岛素抵抗及脂质代谢.

目的:探讨老年人心房颤动病因、临床表现及诊疗方法.方法:对138例老年房颤患者的病例资料进行回顾性分析.结果:18例安装永久心脏起搏器;18例坚持长期服用阿司匹林患者无一例发生脑梗死;房颤伴发脑梗死发生28例、占20.3%,年龄为60～89岁(以70～79岁最多,占46%);1例伴发双下肢动脉栓塞.结论:治疗老年房颤因人而异,抗凝治疗非常重要.

目的:观察稳心颗粒联合普罗帕酮(心律平)治疗老年心律失常的临床疗效及不良反应.方法:将70例经心电图证实有心律失常的老年病人随机分为稳心颗粒与心律平联合应用组(治疗组,40例)与心律平组(对照组,30例),治疗4周.结果:治疗组临床症状缓解总有效率97.5%,治疗组临床症状缓解总有效率优于对照组(P＜0.05),未见明显不良反应;治疗组房性早搏总有效率为77.5%,对室性早搏的总有效率为85.0%,疗效略好于对照组,但与对照组无显著差异(P＞0.05).结论:稳心颗粒与心律平联合应用,可明显改善老年心律失常病人的临床症状,对房性早搏和室性早搏疗效显著.

目的:探讨依那普利对老年无症状性心力衰竭(silent heart tailur,SHF)的心功能改善作用。方法:未经治疗的SHF老年患者91例,随机分为治疗组46例(口服依那普利)及对照组45例(服用安慰剂),治疗16个月,观察两组患者治疗前后心功能(NYHA)分级评估情况和超声心动图心功能测量结果。结果:治疗组46例(100%)治疗后仍然维持心功能Ⅰ级,对照组45例(80%)维持心功能Ⅰ级(P<0.05)。治疗组左心室射血分数LVEF治疗后提高(P<0.05),而对照组下降(P<0.05),两组比较有显著性差异(P<0.05)。左心室收缩末期容积LVESV和左心室舒张末期容积LVEDV,治疗组治疗后明显下降(P<0.05),而对照组治疗后较治疗前上升(P>0.05),两组比较有显著性差异(P<0.05)。结论:依那普利早期干预SHF,可显著改善心功能,延缓老年患者心衰进展,降低住院率。

冠心病心绞痛是常见的心血管疾病之一,发病率和死亡率都有所增高.随着社会老龄化进程加速,老年冠心病患者也逐年增加.为了观察参芎葡萄糖注射液治疗老年心绞痛的临床疗效,我们收集了50例住院老年心绞痛患者的对比治疗情况,总结分析如下.