The effect of sodium chloride concentration,pH,enzyme acting temperature,time and its adding amount on the gel hardness and water holding capacity of egg white treated by transglutaminase were studied in this experiment.The SDS-PAGE electrophoresis was used to analyze the protein crosslinking after enzyme treatment to explore the mechanism of transglutaminase on heat coagulation of egg white.The results showed that the gel hardness and water holding capacity of egg white was increased significnatly after TGase treatment (p ＜ 0.01).When sodium chloride concentration was 0.4mol/L,pH was 6.0,temperature was 35℃,acting time was 3.5h and the TGase addition was 10U/g protein,the value of gel hardness and water holding capacity of egg white reached their maximum value of(134.73 ± 1.79) g and 93.83％ ±0.58％,which were 58.87％ and 29.38％ higher than that of control group,respectively.The SDS-PAGE electrophoresis analysis showed:TGase could contribute to the crosslinking of protein molecules in egg white under certain conditions,which improved the heat coagulation of egg white.

目的 探讨液体复苏对创伤致严重脓毒症和脓毒性休克患者心肌损伤的影响.方法 选取严重脓毒症和脓毒性休克患者26例,均给予液体复苏治疗.根据预后分为2组,治疗3d后存活患者21例为存活组,治疗3d后死亡患者5例为死亡组；根据患者指标达标情况将存活组分为2组,达标患者13例(中心静脉压为8～ 12 mmHg(1 mmHg =0.133kPa),平均动脉压为65 ～ 90 mmHg,混合静脉血氧饱和度为70％以上)为达标组.未达标患者8例为未达标组,分析各组患者间血流动力学指标、组织灌注指标、心肌损伤指标.结果 液体复苏治疗后,患者肺动脉楔压、心指数、体循环血管阻力指数、左心室每搏工作指数、右心室每搏工作指数均显著升高,患者肺循环血管阻力指数、血乳酸浓度、血二氧化碳分压均显著下降(P均＜0.05).存活组与死亡组患者心肌肌钙蛋白Ⅰ、N末端-心室利钠肽前体均显著下降(P均＜0.05).死亡组患者心肌肌钙蛋白Ⅰ、N末端-心室利钠肽前体均明显高于存活组(P均＜0.05).未达标组患者心肌肌钙蛋白Ⅰ、N末端-心室利钠肽前体均明显高于达标组(P均＜0.05).结论 液体复苏可显著改善严重脓毒症和脓毒性休克患者的心肌功能,心肌肌钙蛋白Ⅰ和N末端-心室利钠肽前体等心肌损伤指标与患者的预后有着密切联系.

The research situation, functional characteristics of herbal chicken soup and the research progress of its processing technology were summarized. Combined with market demand, the problems exist-ing in the research of the herbal chicken soup were analyzed and the development trend of its products was forecasted.

The changes of egg pulp’s gel hardness after heating through adding different amount of TGase in the egg pulp are studied in this paper. The result shows that the gel hardness of whole egg pulp increase significantly with the increasing additive amount of Tgase. The maximum gel hardness is 53.18g/mm2 when TGase additive amount is 1.6U/mL.

Texture analyzer was used to discuss the influence of sodium chloride addition,pH value,transglutaminase(TGase)addition,and its action time and temperature on gel hardness of egg pulp after heat treatment.It was aimed to expand the application of TG enzyme in egg industry and provided some data for industrialized production of traditional steamed egg in the future.The results showed TG enzyme could improve the egg pulp gel hardness after heating.The maximum gel hardness 52.375 g of egg pulp after treatment was obtained under following optimal conditions:sodium chloride addition2%,pH value 7,1.2 U/mL of TG,enzyme action temperature30℃and reaction time 4h.

<正>床边紧急心脏起搏是抢救心脏骤停及严重心动过缓的有效措施[1,2]。本文观察了60例心脑血管病急症患者床边紧急心脏起搏,报道如下。临床资料收集2006年1月～2010年3月到我院进行心脏急症治疗的病例120例(男67,女53),年龄39～

Objective To evaluate and analyze the application value of continuous Acute Physiology and Chronic Health EvaluationⅢ(APACHEⅢ)apply on critically ill patients in the intense care unit.MethodsBy collecting and sorting out the data of 320 cases of serious patients,the prognosis of patients were evaluated by APACHEⅢfor 3 days in continuation and tracking the results in the end.ResultsThere was a significant correlation between the score of APACHEⅢ and the actual mortality.The mortality increased remarkably when the score was>90.It is helpful that judging the result of serious patients by using continuous APACHEⅢ.ConclusionsAPACHEⅢ score system can be used to evaluate state of an illness and prognosis.It is the basis of medical quality and reasonable application of ICU source.

[Objective] To apply Acute Physiology Chronic Health Evaluation Ⅲ(APACHEⅢ)in evaluation of the ICU patient's condition and prognosis,and simultaneously compare the prognosis predicted by dynamic APACHEⅢ scoring and MODS scoring with the actual prognosis.[Methods]The clinical data of 286 critically ill patients were collected and divided into survival group (252 cases) and death group (34 cases).APACHEⅢ was adopted to evaluate patients of the 2 groups for the 1st,3rd,5th,7th day,the results were compared.MODS scoring and prognosis prediction were simultaneously carried out.[Results]The APACHEⅢ scores of the 1st,3rd,5th,7th day and fatality rate of death group were all higher than those of the survival group(P<0.05 or P<0.01).Dynamic scoring could help to determine prognosis.There was consistency between APACHE Ⅲ scoring and MODS scoring.MODS scoring was correlated with mortality.[Conclusion]Dynamic APACHEⅢ and MODS scoring systems can be used for evaluation of the ICU patient's condition and prognosis,and it is the evidence of medical quality and rational use of ICU resource.

<正>近年来,为提高心肺脑复苏成功率,我科在心肺脑复苏(cardiopulmonary cerebral resuscitation,CPCR)中联合应用肾上腺素、山莨菪碱及纳洛酮,取得良好效果,现报告如下。

近年来,为提高心肺脑复苏成功率,我科在心肺脑复苏(cardiopulmonary cerebral resuscitation,CPCR)中联合应用肾上腺素、山莨菪碱及纳洛酮,取得良好效果,现报告如下.

[Objective]To evaluate the safety and effectiveness of the tirofiban,which was used in the percutaneous coronary intervention therapy for the patients with acute myocardial infarction with st-segment elevation(STEMI).[Methods] The patients with confirmed STEMI were randomly divided into test group and control group,and the test group received intravenous tirofiban before PCI.The TIMI blood flow before and after PCI,the left ventricular ejection fraction after PCI for 1 week,bleeding complications and the cardiovascular events which occurred in 30 days were observed in both 2 groups.[Results] Compared with the PCI group,the tirofiban group had the higher harvest rate of TIMI Class Ⅲ blood flow,and the difference was significant(P0.05);the left ventricular ejection fraction after PCI for 1 week was higher than the PCI group,and the difference was significant(P0.05);bleeding complications had the tendency of being higher than the PCI group,but the difference was not significant(P0.05),with no sever hemorrhage;the incidence of the main cardiovascular events was lower than the PCI group,and the differences was significant(P0.05).[Conclusion] The tirofiban can improve the TIMI blood flow in the infarct related artery of patients with STEMI,so it can be safely used in the emergency interventional treatment of STEMI,and significantly lower the incidence of the cardiovascular events which occur in 30 days.

Objective To investigate the integratived therapy effect of rhubarb which used in patients with ARDS.Methods 60 patients with ARES were divided randomly into two groups,one is control group which were treated by general treatment and the other is treatment group which were treated by general treatment as well as rhbubarb given through stomach tube.The plasm concentrations of IL-6,IL-8 and blood gas analysis were observed.Results After treatment with rhubarb 7 day,concentrations of IL-6,IL-8,blood gas analysis were improved significantly (P<0.05 or P<0.01) compared to control group;Although blood gas analysis improved significantly(P<0.05 or P<0.01),concentrations of IL-6,IL-8 hadn’t significantly change changed in control group(P>0.05);Treatment with rhubarb hospitalization time and the mortality were significant different(P<0.05 or P<0.01) compared to control group.Conclusion Rhubarb could restrain release of inflammatory media,decrease the mortality,shorten hospitalization time, and improve pulmonary function of patient with ARDS.

目的探讨血管性假血友病因子(vWF)及高敏C反应蛋白(hs-CRP)检测在原发性高血压患者中的临床意义。方法选120例原发性高血压患者及30例正常者空腹取肘静脉血采用酶联免疫吸附双抗体夹心法(ELISA),测定血浆vWF含量;采用免疫比浊法测定其hs-CRP血浆浓度,按原发性高血压分期分组。结果高血压Ⅰ,Ⅱ,Ⅲ期患者vWF含量及高敏C反应蛋白(hs-CRP)水平显著高于正常对照组(P<0.01),且原发性高血压合并有冠心病、糖尿病者vWF,hs-CRP水平显著增高,经系统治疗后,患者vWF含量及hs-CRP水平较发病时显著下降。结论vWF是血管内皮受损的标志物,C反应蛋白(CRP)是组织损伤、炎症反应的标志,高血压患者存在内皮细胞受损及炎症反应,内皮细胞受损及炎症反应程度与病情正相关。系统治疗对稳定原发性高血压病情有利。

2007年7月7日,我们收治了1例超大剂量氯氮平中毒患者,经门诊及住院及时治疗,抢救成功.

感染、炎症、坏死组织、组织缺血和再灌注损伤等因素可导致全身炎症反应综合征(SIRS),炎性细胞因子在炎症反应过程中具有广泛的生理功能,是引起重度感染、感染性休克,多器官功能衰竭(MODS)及死亡的主要原因.凝血功能障碍及血液流变性异常将严重影响危重病患者病情的发展和预后,尽早采取有效措施,保护好凝血功能、改善血液流变性是危重病患者成功抢救的关键之一.中药大黄对危重病患者多个重要脏器有保护作用,能有效预防危重病人发生MODS,对于已发生MODS的患者,大黄具有保护作用,从而降低临床病死率,提高抢救成功率[1].

患者男,60岁.因间歇胸闷6个月入院.有高血压病史5年,最高血压160/90mmHg,口服降压药控制在130/80mmHg左右,无糖尿病史和烟酒嗜好.

Objective To investigate the integrative therapy effect of ambroxol which used as continu intravenous pump or intravenous dripping in patients with acute respiratory distress syndrome(ARDS).Methods 63 patients with ARDS were divided randomly into three groups,control group (group A,n=20) and treatment group(group B,n=21),(group C,n=22).Patients in group A were treated with 0.9% NaCl (200 ml),group B with ambroxol (60 mg) plus 0.9 % NaCl (200 ml) intrvenous dripping once a day for 7 days and group C with ambroxol (60 mg) plas 0.9 % NaCl (200 mg) continue pump 24 hours for 7 days.The plasma concentrations of IL-6,IL-8 were measured and blond gas analysis and pulmonary function index were taken before and after the infusion of 5 days and 7 days respectively.Re- sults Compared to group A and group B after treatment with ambroxol in group C,concentrations of IL-6,IL-8,blood gas analysis and pul- monary function index were improved significantly (P0.05 or P0.01).After treatment with ambroxol 7 day in group B,concentrations of IL-6,IL-8 were decresed distinctively(P0.05) compared to before treatment.In group A blood gas analysis and pulmonary function in- dex were much better than those before treatment and in group A(P0.05 or P0.01).Although blood gas analysis improved significant- ly (P0.05),concentrations of IL-6,IL-8 and pulmonary function index hadn't significantly change in group A (P0.05).Conclusion Ambroxol could restrain release of inflammatory media,decrease ALI and improve pulmonary function of patient with ARDS.Continue in- travenous pump is better than intravenous dripping

目的:探讨大黄对全身炎症反应综合征(SIRS)患者凝血功能的影响机制.方法:36例无凝血功能障碍的SIRS患者随机分为二组,对照组18例给予常规抗炎及支持对症治疗,治疗组18例加用生大黄粉60g/天煎制后分3次鼻饲.分别检测二组患者治疗前、治疗后1、3、7天的血浆凝血酶原时间、血小板计数和血浆血管性假血友病因子(vWF)含量.结果:治疗后治疗组第7天的检测指标与对照组比较有显著差异.结论:大黄对SIRS患者的凝血功能有保护作用.

Objective Brain natriuretic peptide (BNP),Interleukin–6(IL-6) and C-reactive protein(CRP) before and after thrombolysis in acute myocardial infarction(AMI) were measured to explore their change characteristics and clinical significance.Methods Sixty-six patients diagnosed as AMI by coronary angiography received thrombolysis and 53 healthy subjects were as the control.Peripheral Venous blood was taken before and after thrombolysis,BNP,IL-6 and CRP were measured by ELISA.Results The average levels of plasma BNP,IL-6 and CRP in the AMI patients were higher than that in the control group(P 0.01),their levels in AMI patients before thrombolysis were higher than those after thrombolysis(P 0.05).Conclusion BNP,IL-6 and CRP measurement may help us evaluate the prognosis of AMI and the efficacy of thrombolysis.

目的:探讨大黄防治多器官功能不全综合征(MODS)的临床疗效.方法:对收治ICU的615例危重病人,治疗组260例,在常规治疗的基础上给予大黄鼻饲(20g/d),分3～4次;对照组355例,只给予常规(不用大黄)治疗.两组病人的病情及其他治疗方法无差异.统计观察两组病人MODS的发生率及病死率.结果:615例危重病人发生MODS 98例.MODS的发生率治疗组11.15%,对照组19.44%.发生MODS的患者死亡64例.MODS病死率治疗组为48.28%,对照组为72.46%.两组病人MODS的发生率和病死比较具有统计学意义.结论:大黄治疗危重病患者可降低MODS的发生率,提高MODS的抢救成功率.