Anthropogenic activities have triggered a global biodiversity crisis, characterized by severe declines in insect abundance and species richness. Effective conservation is often hindered by the taxonomic impediment and the 'Raunkiæran shortfall' - the limited knowledge regarding species' ecological and functional traits. However, developing targeted conservation strategies requires moving beyond observed patterns toward mechanistic, trait-based frameworks. Here, we examine how several major and emerging threats - biological invasions, wildlife trade, climate change, and light pollution - impact insect communities via complex direct and indirect pathways. Drawing on recent literature, we argue that mechanistic insights facilitate 'precision species conservation', allowing for targeted management strategies tailored to specific ecological vulnerabilities. For example, understanding how artificial light disrupts insect flight enables more sustainable lighting designs that reduce mortality, while identifying trait-based vulnerabilities strengthens biosecurity and trade regulations to limit biological invasions and unsustainable wildlife trade. Because the magnitude of these impacts varies across trophic, morphological, and taxonomic scales, conservation scenarios must be equally specialized. Bridging the gap between mechanistic ecological knowledge and practical application is critical for developing robust frameworks to mitigate global insect biodiversity loss.

Aim Species composition in biological assemblages is driven by an interplay of evolutionary, environmental, and ecological processes. Investigating the relationships between evolutionary history and climatic conditions can shed light on the variation in biodiversity and phylogenetic structure in biological assemblages. Harnessing a global dataset of ant biodiversity, here we determine the extent to which climate drives geographic patterns of phylogenetic diversity and dispersion in ants.Methods We calculated mean pairwise distance (MPD, measuring phylogenetic diversity) and the standardized effect size of MPD (MPDses, measuring phylogenetic dispersion) in each of over 11,500 ant assemblages, and related them to variables representing current climatic conditions and Quaternary climate change.Location Global.Time Period Current.Taxon Ants.Results We find that these two metrics are positively correlated with climate averages yet negatively correlated with climate variability. We also find that current climatic conditions are more strongly associated with these phylogenetic metrics than Quaternary climate change. Temperature variables had stronger associations than precipitation variables, and climate extremes and seasonality affected phylogenetic diversity and dispersion equally. Lastly, areas with higher phylogenetic diversity and dispersion tend to have more paleo-endemic lineages.Main Conclusions The geographic patterns and climatic associations with ant phylogenetic diversity and dispersion found in this study are largely consistent with those found in butterflies and several plant groups, suggesting that the same mechanisms may have driven phylogenetic structure more broadly across the tree of life.

On a global scale, biodiversity is geographically structured into regions of biotic similarity. Delineating these regions has been mostly targeted for tetrapods and plants, but those for hyperdiverse groups such as insects are relatively unknown. Insects may have higher biogeographic congruence with plants than tetrapods due to their tight ecological and evolutionary links with the former, but it remains untested. Here, we develop a global regionalization for a major and widespread insect group, ants, based on the most comprehensive distributional and phylogenetic information to date, and examine its similarity to regionalizations for tetrapods and vascular plants. Our ant regionalization supports the newly proposed Madagascan and Sino-Japanese realms based on tetrapod delineations, and it recovers clusters observed in plants but not in tetrapods, such as the Holarctic and Indo-Pacific realms. Quantitative comparison suggests strong associations among different groups-plants showed a higher congruence with ants than with tetrapods. These results underscore the wide congruence of diverse distribution patterns across the tree of life and the similarities shared by insects and plants that are not captured by tetrapod groups. Our analysis highlights the importance of developing global biogeographic maps for insect groups to obtain a more comprehensive geographic picture of life on Earth. In this study, the authors delineate a global biogeographic map for ants based on their distributions and phylogenetic relationships. They show that current biogeographic maps based on tetrapod groups fail to capture important regions for biodiversity shared by insects and plants, which are both known to possess strong ecological and evolutionary ties.

Forest litter decomposition is an essential component of global carbon and nutrient turnover. Invertebrates play important roles in litter decomposition, but the regional pattern of their effects is poorly understood. We examined 476 case studies across 93 sites and performed a meta-analysis to estimate regional effects of invertebrates on forest litter decomposition. We then assessed how invertebrate diversity, climate and soil pH drive regional variations in invertebrate-mediated decomposition. We found that (1) invertebrate contributions to litter decomposition are 1.4 times higher in tropical and subtropical forests than in forests elsewhere, with an overall contribution of 31% to global forest litter decomposition; and (2) termite diversity, together with warm, humid and acidic environments in the tropics and subtropics are positively associated with forest litter decomposition by invertebrates. Our results demonstrate the significant difference in invertebrate effects on mediating forest litter decomposition among regions. We demonstrate, also, the significance of termites in driving litter mass loss in the tropics and subtropics. These results are particularly pertinent in the tropics and subtropics where climate change and human disturbance threaten invertebrate biodiversity and the ecosystem services it provides.

Tropical forests harbor the richest biodiversity among terrestrial ecosystems, but few studies have addressed the underlying processes of species diversification in these ecosystems. We use the pantropical flowering plant family Annonaceae as a study system to investigate how climate and biogeographic events contribute to diversification. A super-matrix phylogeny comprising 835 taxa (34% of Annonaceae species) based on eight chloroplast regions was used in this study. We show that global temperature may better explain the recent rapid diversification in Annonaceae than time and constant models. Accelerated accumulation of niche divergence (around 15 Ma) lags behind the increase of diversification rate (around 25 Ma), reflecting a heterogeneous transition to recent diversity increases. Biogeographic events are related to only two of the five diversification rate shifts detected. Shifts in niche evolution nevertheless appear to be associated with increasingly seasonal environments. Our results do not support the direct correlation of any particular climatic niche shifts or historical biogeographical event with shifts in diversification rate. Instead, we suggest that Annonaceae diversification can lead to later niche divergence as a result of increasing interspecific competition arising from species accumulation. Shifts in niche evolution appear to be associated with increasingly seasonal environments. Our results highlight the complexity of diversification in taxa with long evolutionary histories.

Brief introduction: What are microclimates and why are they important?Microclimate science has developed into a global discipline. Microclimate science is increasingly used to understand and mitigate climate and biodiversity shifts. Here, we provide an overview of the current status of microclimate ecology and biogeography in terrestrial ecosystems, and where this field is heading next. Microclimate investigations in ecology and biogeography: We highlight the latest research on interactions between microclimates and organisms, including how microclimates influence individuals, and through them populations, communities and entire ecosystems and their processes. We also briefly discuss recent research on how organisms shape microclimates from the tropics to the poles. Microclimate applications in ecosystem management: Microclimates are also important in ecosystem management under climate change. We showcase new research in microclimate management with examples from biodiversity conservation, forestry and urban ecology. We discuss the importance of microrefugia in conservation and how to promote microclimate heterogeneity. Methods for microclimate science: We showcase the recent advances in data acquisition, such as novel field sensors and remote sensing methods. We discuss microclimate modelling, mapping and data processing, including accessibility of modelling tools, advantages of mechanistic and statistical modelling and solutions for computational challenges that have pushed the state-of-the-art of the field. What's next?We identify major knowledge gaps that need to be filled for further advancing microclimate investigations, applications and methods. These gaps include spatiotemporal scaling of microclimate data, mismatches between macroclimate and microclimate in predicting responses of organisms to climate change, and the need for more evidence on the outcomes of microclimate management.

Aim Tropical rainforests harbour the richest biodiversity among terrestrial ecosystems, but few studies have addressed underlying processes of species diversification in these ecosystems. We use the pantropical and early divergent flowering plant family Annonaceae as a model system to investigate how abiotic factors such as climate and biogeographic events contribute to the diversification process and lead to its high diversity across a long evolutionary history. Location Tropics and subtropics Taxon Annonaceae Methods A super-matrix was constructed for 835 taxa (34% of Annonaceae species), based on eight chloroplast regions. To understand the patterns of diversification, we reconstructed climatic niche evolution and historical biogeographical events, and tested their association with diversification rates. Results The analysis of temperature-dependent models in Annonaceae lineages provides strong support for the significant influence of global temperature on net diversification and accumulation of species diversity. The pattern of lineage accumulation in the initial radiation is better aligned with the “museum model,” followed by later accumulation consistent with the “recent cradle model” from the late Oligocene to the present. The increase in the diversification rate of the family (around 25 Ma) lags behind the accumulation of niche divergences (around 15 Ma). Biogeographic events are related to only two of the five diversification rate shifts detected. While no direct relationship to shifts in the diversification rate was uncovered, shifts in niche evolution appear to be associated with increasingly seasonal environments. Main Conclusions Global temperature plays a crucial role in driving recent rapid diversification in the Annonaceae. Our study challenges the prevailing assumption of the “museum model” alone and proposes instead a transition from the “museum model” to the “recent cradle model” during the diversification history of the family. However, our findings do not support the direct correlation of any particular climatic niche shifts or historical biogeographical events with shifts in diversification rate. Instead, Annonaceae diversification can lead to later niche divergence as a result of increasing interspecific competition arising from species accumulation. The evolutionary direction of niche shifts furthermore provides insight into the future expansion of Annonaceae into temperate regions. Our results highlight the complexity of the diversification process in taxa with long evolutionary histories, indicating that identifying isolated driving factors is simplistic and inadequate for explaining the observed patterns. Further comprehensive analyses of range evolution are necessary to delve deeper into the interplay among key opportunities, key innovation, and species diversification.

Dengue fever, a mosquito-borne fatal disease, brings a huge health burden in tropical regions. With global warming, rapid urbanization and the expansion of mosquitoes, dengue fever is expected to spread to many subtropical regions, leading to increased potential health risks on local populations. So far, limited studies assessed the dengue fever risk spatially for subtropical non-endemic regions hindering the development of related public health management. Therefore, we proposed a spatial hazard-exposure-vulnerability assessment framework for mapping the dengue fever risk in Hong Kong. Firstly, the spatial distribution of the habitat suitability for Aedes albopictus, the mosquito proxy for the dengue fever hazard, was predicted using a species distribution model (e.g., MaxEnt) relying on a list of variables related to local climate, urban morphology, and landscape metrics. Secondly, the spatial autocorrelation between high dengue hazard and high human popula-tion exposure in urban areas was measured. Finally, the dengue fever risk was assessed at community scale by integrating the results of vulnerability analysis basing on census data. This approach allowed the identification of 17 high-risk spots within Hong Kong. The landscape metrics about land utilities and vegetations, and urban morphological characteristics are the influential factors on the spatial distribution of dengue vector. In addition, the underlying factors behind each hot spot were investigated, and specific suggestions for dengue prevention were proposed accordingly. The findings provide a useful reference for developing local dengue fever risk pre-vention measures, with the proposed method easily exportable to other high-density cities within subtropical Asia and elsewhere.

Forest litter decomposition is an essential component of global carbon and nutrient turnover. Soil invertebrates play important roles in litter decomposition, but the regional pattern of their effects is poorly understood. We examined 476 case studies across 93 sites and performed a meta-analysis to estimate regional effects of invertebrates on forest litter decomposition. We then assessed how invertebrate diversity, climate and soil pH drive regional variations in invertebrate-mediated decomposition. We found that (1) invertebrate contributions to litter decomposition are 1.5 times higher in tropical forests than forests outside the tropics, with an overall contribution of 31% to global forest litter decomposition; and (2) invertebrate diversity, particularly of termites, together with warm, humid and acidic environments in the tropics are positively associated with forest litter decomposition by soil invertebrates. Our results demonstrate the significant difference in invertebrate effects on mediating forest litter decomposition between the tropics and elsewhere. We demonstrate, also, the significance of termites in driving litter mass loss in the tropics. These results are particularly pertinent in the tropics where climate change and human disturbance threaten soil invertebrate biodiversity and the ecosystem services it provides.

Dengue fever is a mosquito-borne disease caused by the dengue virus bringing huge health burdens in tropical regions. With global warming, rapid urbanization, and mosquito species introductions, the range of dengue fever is expected to expand to subtropical regions and increase potential health risks for local populations. To reduce dengue fever transmission, relevant risk map is one of the most effective tools for public health management. Though there is abundant literature about mapping the dengue fever risks in endemic regions, few studies in contrast have investigated dengue fever risks for non-endemic regions; hindering the development of preparedness planning.In this study, the spatial hazard-exposure-vulnerability assessment framework proposed by the Intergovernmental Panel on Climate Change was applied in to detect the dengue fever risk in Hong Kong, which is a typical high-density city located within a subtropical region. Firstly, the spatial distribution of the habitat suitability for Aedes albopictus, a mosquito species common in Hong Kong and proxy for the potential dengue fever hazard, was predicted using MaxEnt models relying on the surveillance data and a list of variables related to urban morphology, landscape, land utilization, and local climate. Secondly, the bivariate local Moran’s I was measured to identify urban areas with both high dengue hazard and high human population exposure. Then, vulnerable groups among the human population were identified from the 2016 Hong Kong census data. Finally, dengue risks were assessed at the community scale by overlapping the results of hazard, exposure, and vulnerability analysis.In the optimal MaxEnt model predicting the presence possibility of Aedes albopictus, the normalized difference vegetation index, frontal area index, and the aggregation index of public residential land ranked the top three among all predictors, with permutation importance of 31.8%, 22.8%, and 17% respectively. Three components were generated after principal component analysis on the vulnerable groups. Lastly, this approach allowed the identification of 17 high-risk spots within Hong Kong. In addition, the underlying factors behind each hot spot were investigated from the aspects of hazard, exposure, and vulnerability respectively, and specific suggestions for dengue prevention were proposed accordingly.The findings provide a useful reference for developing local dengue fever risk prevention measures, with the proposed method easily exportable to other high-density cities within subtropical Asia and elsewhere.This study was funded by the Health and Medical Research Fund of the Food and Health Bureau (No. 20190672).

Aim: Biogeographical regionalization is scant for most insect groups due to shortfalls in distribution and phylogenetic information, namely the Wallacean and Darwinian shortfalls respectively. Here, we focused on the European ants and compared new techniques to classical analyses based on regional lists and taxonomic methods. We asked the following: (1) Can grid-based regionalizations using novel distribution data improve biogeographical transitions? and (2) Can phylogenetic approaches reveal new insights regarding ant evolutionary history? Location: Europe and Anatolia. Taxon: Ants (Formicidae). Methods: First, we developed a refined database integrating the occurrences of 747 ant species across 207 regions of Europe and Anatolia, based on newly expert-validated records derived from the existing Global Ant Biodiversity Informatics database. Using range estimates for these species derived from polygons and species distribution modelling, we produced species assemblages in 50 x 50 km grid cells. We calculated taxonomic and phylogenetic turnover of ant assemblages, then performed a hierarchical clustering procedure to delineate biogeographical structure. Results: At both the regional list and grid assemblage levels, the Mediterranean has higher turnover and more biogeographical regions than northern Europe, both taxonomically and phylogenetically. Delineations based on grid assemblages detected more detailed biogeographical transitions, while those based on regional lists showed stronger insularity in biogeographical structure. The phylogenetic regionalization suggested a very similar spatial structure but varied affinities between assemblages in comparison to the taxonomic approach. Main Conclusions: Here, we integrated expert-validated regional lists, species distribution modelling and a recent phylogeny to tackle Wallacean and Darwinian shortfalls for an important insect group by developing a next-generation map of biogeographical regionalization for European ants. The results of this study suggest strong constraints from geographical barriers and potential effects of climatic history on ant distributions and evolutionary history, and also provide baseline spatial information for future investigations of regional insect distributions.

Understanding mechanistic processes driving biodiversity change in land‐use transformation is paramount but challenging. Functional trait‐based approach provides a framework to understand the environmental filtering process of species composition shifts. Yet, trait selection bias may affect the results on forecasting species dynamics, subsequent analyses and the scope of conclusions, in particular, if most studies on terrestrial arthropods rely exclusively on the use of a subset of morphological traits. The integration of ecophysiological traits could then provide additional dimensions of environmental filtering caused by land‐use conversion. Our study investigates how land‐use conversion from forest to rubber plantation impacts functional traits distribution and trait space in foraging ant assemblages in tropical Thailand by integrating both physiological and morphological traits. The results showed that both physiological and morphological traits may explain, although unevenly, the differences observed in ant assemblages between forest and rubber plantation. The use of Critical Thermal maximum provided an important and unique role for differentiating assemblages encountered in both habitats and in characterising the niche structure of species assemblage from forest. The use of particular traits can thus improve our perception of species dynamic along land‐use gradient. To advance our knowledge on the species responses and consequences of land‐use changes, the use of different types of functional traits is recommended.

Research in global change ecology relies heavily on global climatic grids derived from estimates of air temperature in open areas at around 2 m above the ground. These climatic grids do not reflect conditions below vegetation canopies and near the ground surface, where critical ecosystem functions occur and most terrestrial species reside. Here, we provide global maps of soil temperature and bioclimatic variables at a 1‐km 2 resolution for 0–5 and 5–15 cm soil depth. These maps were created by calculating the difference (i.e. offset) between in situ soil temperature measurements, based on time series from over 1200 1‐km 2 pixels (summarized from 8519 unique temperature sensors) across all the world's major terrestrial biomes, and coarse‐grained air temperature estimates from ERA5‐Land (an atmospheric reanalysis by the European Centre for Medium‐Range Weather Forecasts). We show that mean annual soil temperature differs markedly from the corresponding gridded air temperature, by up to 10°C (mean = 3.0 ± 2.1°C), with substantial variation across biomes and seasons. Over the year, soils in cold and/or dry biomes are substantially warmer (+3.6 ± 2.3°C) than gridded air temperature, whereas soils in warm and humid environments are on average slightly cooler (−0.7 ± 2.3°C). The observed substantial and biome‐specific offsets emphasize that the projected impacts of climate and climate change on near‐surface biodiversity and ecosystem functioning are inaccurately assessed when air rather than soil temperature is used, especially in cold environments. The global soil‐related bioclimatic variables provided here are an important step forward for any application in ecology and related disciplines. Nevertheless, we highlight the need to fill remaining geographic gaps by collecting more in situ measurements of microclimate conditions to further enhance the spatiotemporal resolution of global soil temperature products for ecological applications.

Plant functional traits represent adaptive strategies to the environment, linked to biophysical and biogeochemical processes and ecosystem functioning. Compilations of trait data facilitate research in multiple fields from plant ecology through to land-surface modelling. Here we present version 2 of the China Plant Trait Database, which contains information on morphometric, physical, chemical, photosynthetic and hydraulic traits from 1529 unique species in 140 sites spanning a diversity of vegetation types. Version 2 has five improvements compared to the previous version: (1) new data from a 4-km elevation transect on the edge of Tibetan Plateau, including alpine vegetation types not sampled previously; (2) inclusion of traits related to hydraulic processes, including specific sapwood conductance, the area ratio of sapwood to leaf, wood density and turgor loss point; (3) inclusion of information on soil properties to complement the existing data on climate and vegetation (4) assessments and flagging the reliability of individual trait measurements; and (5) inclusion of standardized templates for systematical field sampling and measurements.

Knowledge on the distribution and abundance of organisms is fundamental to understanding their roles within ecosystems and their ecological importance for other taxa. Such knowledge is currently lacking for insects, which have long been regarded as the "little things that run the world". Even for ubiquitous insects, such as ants, which are of tremendous ecological significance, there is currently neither a reliable estimate of their total number on Earth nor of their abundance in particular biomes or habitats. We compile data on ground-dwelling and arboreal ants to obtain an empirical estimate of global ant abundance. Our analysis is based on 489 studies, spanning all continents, major biomes, and habitats. We conservatively estimate total abundance of ground-dwelling ants at over 3 × 1015 and estimate the number of all ants on Earth to be almost 20 × 1015 individuals. The latter corresponds to a biomass of ∼12 megatons of dry carbon. This exceeds the combined biomass of wild birds and mammals and is equivalent to ∼20% of human biomass. Abundances of ground-dwelling ants are strongly concentrated in tropical and subtropical regions but vary substantially across habitats. The density of leaf-litter ants is highest in forests, while the numbers of actively ground-foraging ants are highest in arid regions. This study highlights the central role ants play in terrestrial ecosystems but also major ecological and geographic gaps in our current knowledge. Our results provide a crucial baseline for exploring environmental drivers of ant-abundance patterns and for tracking the responses of insects to environmental change.

Leaf morphological traits vary systematically along climatic gradients. However, recent studies in plant functional ecology have mainly analysed quantitative traits, while numerical models of species distributions and vegetation function have focused on traits associated with resource acquisition; both ignore the wider functional significance of leaf morphology. A dataset comprising 22 leaf morphological traits for 662 woody species from 92 sites, representing all biomes present in China, was subjected to multivariate analysis in order to identify leading dimensions of trait covariation (correspondence analysis), quantify climatic and phylogenetic contributions (canonical correspondence analysis with variation partitioning) and characterise co-occurring trait syndromes (k-means clustering) and their climatic preferences. Three axes accounted for >20% of trait variation in both evergreen and deciduous species. Moisture index, precipitation seasonality and growing-season temperature explained 8%-10% of trait variation; family 15%-32%. Microphyll or larger, mid- to dark green leaves with drip tips in wetter climates contrasted with nanophyll or smaller glaucous leaves without drip tips in drier climates. Thick, entire leaves in less seasonal climates contrasted with thin, marginal dissected, aromatic and involute/revolute leaves in more seasonal climates. Thick, involute, hairy leaves in colder climates contrasted with thin leaves with marked surface structures (surface patterning) in warmer climates. Distinctive trait clusters were linked to the driest and most seasonal climates, for example the clustering of picophyll, fleshy and succulent leaves in the driest climates and leptophyll, linear, dissected, revolute or involute and aromatic leaves in regions with highly seasonal rainfall. Several trait clusters co-occurred in wetter climates, including clusters characterised by microphyll, moderately thick, patent and entire leaves or notophyll, waxy, dark green leaves. Synthesis. The plastic response of size, shape, colour and other leaf morphological traits to climate is muted, thus their apparent shift along climate gradients reflects plant adaptations to environment at a community level as determined by species replacement. Information on leaf morphological traits, widely available in floras, could be used to strengthen predictive models of species distribution and vegetation function.

ABSTRACTAimBiogeographic regionalization has fascinated biogeographers and ecologists for centuries and is endued with new vitality by evolutionary perspectives. However, progress is scant for most insect groups due to shortfalls in distribution and phylogenetic information, namely Wallacean and Darwinian shortfalls respectively. Here, we used the western Palearctic ants as the case to tackle these shortfalls and test their biogeographic structure through novel distribution data and phylogenetic approaches.LocationWestern Palearctic realm.TaxonAnts (Formicidae).MethodsFirstly, we developed a refined database integrating the occurrences of 747 ant species across 207 regions of the western Palearctic realm, based on newly expert-validated records derived from the existing global ant biodiversity informatics. Using range estimates for these species derived from polygons and species distribution modelling, we produced species assemblages in 50 × 50 km grid cells. We calculated taxonomic and phylogenetic turnover of ant assemblages, performing hierarchical clustering analysis using the Simpson dissimilarity index to delineate biogeographic structure.ResultsAt both the regional list- and grid assemblage-levels, the Mediterranean has higher turnover and more biogeographic regions than northern Europe, both taxonomically and phylogenetically. Delineations based on grid assemblages detected more detailed biogeographic transitions, while those based on regional lists showed stronger insularity in biogeographic structure. The phylogenetic regionalization suggested closer but varied affinities between assemblages in comparison to the taxonomic approach.Main conclusionsHere, we integrated expert-validated regional lists, species distribution modelling, and a recent phylogeny to tackle Wallacean and Darwinian shortfalls for an important insect group by developing a next-generation map of biogeographic regionalization for the western Palearctic ants. The results of this study suggest strong constraints from geographic barriers and potential effects of climatic history on ant distributions and evolutionary history, and also provide baseline spatial information for future investigations of regional insect distributions.

Mountains harbour a rich and non‐random cluster of species, yet knowledge of the species' biological attributes that support their coexistence in the montane community is limited. Here, we investigated the association of species occurrence on the Tibetan Plateau with their morphological, ecological or evolutionary constraints.

Background As global climate change accelerates, ecologists and conservationists are increasingly investigating changes in biodiversity and predicting species distribution based on species observed at sites, but rarely consider those plant species that could potentially inhabit but are absent from these areas (i.e., the dark diversity and its distribution). Here, we estimated the dark diversity of vascular plants in China and picked up threatened dark species from the result, and applied maximum entropy (MaxEnt) model to project current and future distributions of those dark species in their potential regions (those regions that have these dark species). Methods We used the Beals probability index to estimate dark diversity in China based on available species distribution information and explored which environmental variables had significant impacts on dark diversity by incorporating bioclimatic data into the random forest (RF) model. We collected occurrence data of threatened dark species (Eucommia ulmoides, Liriodendron chinense, Phoebe bournei, Fagus longipetiolata, Amentotaxus argotaenia, and Cathaya argyrophylla) and related bioclimatic information that can be used to predict their distributions. In addition, we used MaxEnt modeling to project their distributions in suitable areas under future (2050 and 2070) climate change scenarios. Results We found that every study region’s dark diversity was lower than its observed species richness. In these areas, their numbers of dark species are ranging from 0 to 215, with a generally increasing trend from western regions to the east. RF results showed that temperature variables had a more significant effect on dark diversity than those associated with precipitation. The results of MaxEnt modeling showed that most threatened dark species were climatically suitable in their potential regions from current to 2070. Discussions The results of this study provide the first ever dark diversity patterns concentrated in China, even though it was estimated at the provincial scale. A combination of dark diversity and MaxEnt modeling is an effective way to shed light on the species that make up the dark diversity, such as projecting the distribution of specific dark species under global climate change. Besides, the combination of dark diversity and species distribution models (SDMs) may also be of value for ex situ conservation, ecological restoration, and species invasion prevention in the future.

Larva of Vestalaria venusta is identified using DNA barcoding match with the adult and described in the first time. Morphological characters are compared with those of Matrona basilaris and Vestalis amoena. The validity of genus the Vestalaria is reconfirmed. The important role of DNA barcoding in odonate larva identification is emphasized.