目的:系统评价火针与普通针刺对脑卒中后肩手综合征的康复疗效.方法:检索万方(WanFang)、中国知网(CNKI)、维普(VIP)、中国生物医学文献服务数据系统(SinoMed)、PubMed、EMbase、Web of Science、the Cochrane Library、EBSCO、OVID等数据库中火针与普通针刺治疗脑卒中后肩手综合征康复疗效比较的临床随机对照试验研究,检索时限为自建库至 2022 年 7 月 31 日.经筛选后对最终纳入的文献进行质量评价.采用 RevMan 5.4软件进行 Meta分析.结果:纳入文献 11 篇,涉及脑卒中后肩手综合征病人 729例.通过 Meta分析发现,火针对比普通针刺,肩手综合征评估量表(SHSS)评分[MD=-1.45,95%CI(-1.64,-1.26),P<0.000 01]、临床疗效[OR=2.68,95%CI(1.57,4.59),P<0.000 3]、Fugl-Meyer运动功能评分上肢部分[MD=6.01,95%CI(3.18,8.85),P<0.001],肩关节外展活动度[MD=13.11,95%CI(8.34,17.87),P<0.000 01]差异均有统计学意义.结论:现有证据表明,火针较普通针刺能更有助于脑卒中后肩手综合征病人的康复,且安全性较高.

The purpose of this study was to determine the effect of amiodarone (Ami) on hERG-T618I currents in HEK293 cells. A transient transfection method was used to transfer hERG-T618I and hERG wild-type (WT) channel plasmids into HEK293 cells. An extracellular local perfusion method was used for administration. Currents were recorded using the whole-cell patch clamp technique. Ami (10 μM) had a greater retarding effect on the hERG-T618I channel than WT (P < 0.05). The half-inhibitory concentration for the mutant was approximately 1.82 times that of WT (P < 0.05). The WT current inhibition fraction against Ami was significantly greater than T618I in the same cell (P < 0.05). The STEP current of the mutant channel was larger than the WT channel, but the characteristic of inward rectification did not appear. Ami reduced the STEP current of the mutant channel, and the steady-state activation curve indicated that channel activation decreased (P > 0.05). Ami restored partial inactivation of the mutant channel. Ami effectively reduced the current in the phase 2 plateau (P < 0.05), but the phase 3 current did not exhibit the characteristics of a WT current. Ami inhibited hERG-T618I currents on HEK293 cells, but the effect was weaker than WT.

Objective To investigate the protective effect and mechanism of pretreatment of Gualou Xiebai Banxia decoction on myocardial ischemia-reperfusion injury(MIRI)model rats.Methods This experiment was conducted from February 2022 to February 2023.A total of 40 rats were divided into sham group(A group),model group(B group),Gualou Xiebai Banxia decoction group(C group),and nuclear factor-κB(NF-κB)inhibitor group(D group)using random number table method,10 rats in each group.Rats in groups C and D were pretreated with Gualou Xiebai Banxia decoction,while rats in groups A and B were pretreated with an equal amount of 0.9%sodium chloride solution.All four groups were continuously intervened for 7 days.Rats in group A underwent thoracotomy at 1 hour after the last administration,but were not ligated;while rats in groups B,C,and D were used to prepare MIRI models using the left anterior descending branch ligation method at 1 hour after the last administration.Rats in group D were intraperitoneally injected with ammonium pyrrolidine dithioformate at 10 minutes before reperfusion.The percentage of myocardial infarction area,myocardial tissue morphology,serum inflammatory factor(IL-1β and TNF-α)levels,serum oxidative stress indicators[malondialdehyde(MDA),superoxide dismutase(SOD),lactate dehydrogenase(LDH)],myocardial tissue ultrastructure,Toll like receptor 4(TLR4),NF-κB p65 protein expression level and relative mRNA expression level in myocardial tissue were compared in the four groups of rats.Results The percentage of myocardial infarction area in group B was higher than that in group A,the percentage of myocardial infarction area in groups C and D was lower than that in group B,while the percentage of myocardial infarction area in group D was higher than that in group C(P<0.05).The HE staining results showed that the fiber arrangement,morphological changes,and transverse structural changes of myocardial tissue in group C were superior to those in group B,and the nuclei of group D were scattered and obvious on the outer side.The results of Masson staining showed that the arrangement of myocardial cells in group B was disordered,and a few collagen fibers were positively stained;the arrangement of cells in groups C and D was relatively regular,with almost no positive staining of collagen fibers.Serum levels of IL-1β,TNF-α,MDA and serum LDH activity in group B were higher than those in group A,while the serum SOD activity was lower than that in group A(P<0.05);serum levels of IL-1β,TNF-α,MDA and serum LDH activity in groups C and D were lower than those in group B,while the serum SOD activity was higher than that in group B(P<0.05);serum levels of IL-1β,TNF-α,MDA and serum LDH activity in group D were lower than those in group C,while the serum SOD activity was higher than that in group C(P<0.05).The scanning results of the projection electron microscope showed that there were many vacuoles and autophagosomes in the cytoplasm of group B,a few autophagosomes in the cytoplasm of group C,and partial cytoplasmic vacuolization of group D.The protein expression level and mRNA relative expression level of TLR4 and NF-κB p65 in myocardial tissue of group B were higher than those of group A,the protein expression level and mRNA relative expression level of TLR4 and NF-κB p65 in myocardial tissue of groups C and D were lower than those of group B,while the protein expression level and mRNA relative expression level of TLR4 and NF-κB p65 in myocardial tissue of group D were lower than those of group C(P<0.05).Conclusion Pretreatment of Gualou Xiebai Banxia decoction can reduce inflammatory response,oxidative stress,and inhibit the production of autophagosomes in myocardial cells,thereby reducing the myocardial infarction area and protecting myocardial cells in MIRI model rats.Its mechanism may be related to the inhibition of TLR4/NF-κB signal pathway by Gualou Xiebai Banxia decoction.

Background:This study aimed to explore the effects of Kangdaxin oral liquid on myocardial fibrosis in heart failure with preserved ejection fraction (HFpEF) rats.Methods:A total of 30 Sprague Dawley (SD) rats were randomly divided into 3 groups Sham operation group (Sham), HFpEF group (HFpEF), and HFpEF with drug intervention group (HFpEF + I). Rats in HFpEF + I group were given Kangdaxin oral liquid at a dose of 2.7 mL/(kg·d). After modeling or treatment, the value of E/A and E/e' in each group of rats were measured by echocardiography. The N-terminal pro b-type natriuretic peptide (NT-proBNP) level was determined by enzyme-linked immunosorbent assay (ELISA). Heart weight/body mass index (Hw/W) and left ventricular weight/body mass index (LVw/W) were calculated after the rats were sacrificed; the transforming growth factor-β1 (TGF-β1) protein expression level in cardiac tissue was detected by western blot.Results:Compared with sham group, the values of diastolic function item (E/A) and mitral annular early diastolic velocity (E/e') in HFpEF group were significantly decreased, and the level of NT-proBNP was significantly increased (P<0.05). Compared with HFpEF group, the value of E/A and E/e' in HF + I group were significantly increased, and the level of NT-proBNP was significantly decreased (P<0.05). Compared with sham group, the expression of TGF-β1 protein in heart tissue of HFpEF group were significantly increased (P<0.05). Compared with HFpEF group, the expression of TGF-β1 protein in HFpEF + I group were significantly decreased (P<0.05).Conclusions:Kangdaxin oral liquid has protective effect on heart in HFpEF rats, which can reduce the protein expression of TGF-β1 in the heart tissue of HFpEF rats. This may be a possible mechanism to inhibit myocardial fibrosis and improve cardiac diastolic function.

目的:探讨康达心口服液对缺血性心肌病患者血清人可溶性生长刺激表达基因蛋白2(sST2)水平的影响.方法:收集在福建中医药大学附属人民医院就诊的阳虚水泛型缺血性心肌病患者60例,按随机数字表法分为对照组和观察组各30例,对照组参照《稳定性冠心病诊断与治疗指南2018》和《中国心力衰竭诊断和治疗指南2018》推荐的治疗方案治疗,观察组在对照组基础上加用康达心口服液治疗,两组观察期均为4周,两组治疗前、后分别进行心脏彩超、6分钟步行试验,检测sST2、N末端B型利钠肽原(NT-proBNP)等指标.结果:两组患者治疗前各观察指标无统计学差异(P均＞0.05),用药4周后观察组与对照组比较sST2、NT-proBNP下降,LVEF、6分钟步行试验评级提高,差异有统计学意义(P均＜0.05).结论:联合使用康达心口服液能在优化治疗基础上进一步降低阳虚水泛型缺血性心肌病患者sST2,起到抑制心肌纤维化作用,治疗效果好.

目的 观察双清消斑饮加八段锦联合西药治疗稳定性冠心病痰瘀互结证的疗效.方法 选取2020年5—11月在福建中医药大学附属人民医院心血管科住院的稳定性冠心病痰瘀互结证患者80例,按随机数字表法分为对照组和观察组各40例,研究期间对照组剔除5例,观察组剔除4例,最终对照组35例、观察组36例纳入统计.对照组给予规范西药治疗,观察组在对照组治疗基础上加用双清消斑饮口服联合八段锦训练,2组疗程均为8周.比较治疗前后2组心肺运动试验(CPET)指标[峰值摄氧量(peak VO2)、无氧阈值(AT)、氧脉搏(VO2/HR)、峰值运动时间(peak TIME)、AT时最大代谢当量(MET)]、心绞痛严重程度分级及中医证候积分的变化情况.结果 治疗后2组peak VO2、AT、VO2/HR、peak TIME和MET均较治疗前升高(P均<0.05),治疗后观察组上述指标比对照组升高更明显(P<0.05);2组治疗后心绞痛严重程度分级分布情况均较治疗前改善(P均<0.01),观察组较对照组改善更明显(P<0.05);2组治疗后胸闷痛位置固定、痰多体胖,身体有瘀点或瘀斑、面色紫暗、脘腹痞满、头昏多寐、大便黏腻中医证候积分均较治疗前下降(P均<0.01),治疗后观察组上述指标均较对照组下降更明显(P均<0.05).结论 双清消斑饮加八段锦联合西药治疗稳定性冠心病痰瘀互结证能够改善CPET指标,降低心绞痛严重程度,改善临床症状.

目的:观察化痰活血方对痰瘀互结型冠状动脉慢血流(CSF)患者相关炎症因子、运动平板试验指标以及中医证候的影响.方法:纳入2019年8月-2020年7月在福建中医药大学附属人民医院心血管科住院符合诊断的患者88例,随机分为对照组、中药组各44例,对照组给予西药治疗,中药组在对照组基础上加用化痰活血方,治疗周期为8周,对比治疗前后两组炎症因子、运动平板试验指标以及中医证候积分的变化.结果:治疗后2组炎症因子TNF-α、IL-6均较前明显下降(P＜0.05),中药组下降优于对照组(P＜0.05);治疗后2组运动平板试验指标均较治疗前改善(P＜0.05),中药组明显优于对照组(P＜0.05);治疗后两组患者中医证候积分均较治疗前明显下降(P＜0.05),中药组明显优于对照组(P＜0.01).结论:化痰活血方联合西药治疗能够降低CSF相关炎症因子水平,提高患者运动平板实验阈值,改善中医证候.

目的 探讨高血压病患者盐摄入量与中医证型以及心、肾靶器官损害程度的相关性.方法 纳入符合诊断的高血压病患者148例,填写问卷调查,采集临床资料,进行中医辨证分型.通过24小时尿钠排泄量估算每日盐摄入量,测定尿微量白蛋白(MAU)水平,同时应用心脏彩超测定左心室重量指数(LVMI),进行相关性分析.结果 ①盐摄入量越多,血压分级越高.高血压3级组患者盐摄入量明显高于高血压1级组(P<0.05).②根据盐摄入量分组,高盐组与中盐组SBP、LVMI、MAU明显高于低盐组(P<0.05),高盐组SBP、LVMI、MAU明显高于中盐组(P<0.05).③高血压中医证型中痰湿壅盛组比例较高;高血压病各中医证型患者盐摄入量比较:肝火亢盛证

目的 探讨益气畅脉饮加减治疗心肌桥(气虚血瘀型)的临床疗效和安全性.方法 选取我院心血管科2019年10月至2020年12月的住院患者60例,经冠状动脉造影术检查诊断为心肌桥,证属中医气虚血瘀型,随机分为对照组(美托洛尔缓释片23.75-47.5mg qd,或地尔硫卓片30mg bid-tid治疗)30例和治疗组(益气畅脉饮加减治疗)30例.治疗8周后比较两组治疗效果及药物不良反应.结果 治疗组治疗后中医证候积分下降,与治疗前及对照组治疗后比较均有统计学差异(P＜0.05);比较心绞痛临床症状改善总有效率情况,治疗组高于对照组(P＜0.05);比较心电图疗效情况,治疗组治疗后优于治疗前及对照组治疗后(P＜0.05),治疗过程中未出现不良反应.结论 益气畅脉饮加减治疗气虚血瘀型心肌桥临床效果明显,能够有效改善患者症状,安全性可靠.

目的:观察三步一顺推拿法联合针刺治疗急性腰肌扭伤的早期临床疗效.方法:选取急性腰肌扭伤患者67例,随机分为观察组和对照组,观察组(n=34)采用三步一顺推拿法联合针刺疗法,对照组(n=33)采用针刺疗法.疗程结束后记录两组患者VAS评分、ODI评分及治疗总有效率.结果:治疗前两组患者VAS评分、ODI评分比较差异无统计学意义(P＞0.05),治疗后两组患者VAS评分、ODI评分均显著降低,且观察组降低幅度大于对照组(P＜0.05).观察组患者总有效率为94.12％,高于对照组的84.85％,差异有统计学意义(P＜0.05).结论:三步一顺推拿法联合针刺治疗急性腰肌扭伤早期疗效较好,可有效改善患者临床症状,优于常规针刺疗法.

目的 探讨温阳通络方对急性心肌梗死(AMI)经皮冠状动脉介入治疗(PCI)术后患者心室重构和血管内皮功能的影响.方法 简单随机选取2019年1月-2021年1月在该院接受PCI治疗的AMI患者80例,以随机数表法分为对照组与观察组,各40例.对照组接受阿司匹林,氯吡格雷+阿托伐他汀治疗,观察组加用温阳通络方治疗,比较两组临床疗效及心室重构和血管内皮功能的变化.结果 观察组治疗总有效率高于对照组(92.50％vs 75.00％),差异有统计学意义(x2=4.501,P=0.034);观察组治疗后胸闷、气短与心悸积分低于对照组,差异有统计学意义(P＜0.05);观察组治疗后左室舒张末内径(LVEDD)小于对照组,左室射血分数(LVEF)与室间隔厚度(IVSd)大于对照组,差异有统计学意义(P＜0.05);观察组治疗后血清基质金属蛋白酶-9(MMP-9)与肿瘤坏死因子-α(TNF-α)水平低于对照组,差异有统计学意义(P＜0.05),一氧化氮(NO)水平高于对照组,差异有统计学意义(P＜0.05).结论 温阳通络方可改善AMI患者接受PCI术后心室重构与血管内皮功能.

经络气血运行与黄元御"一气周流"理论之脏腑气机升降有许多共通之处,故从整体辨证出发,根据"一气周流"理论运用针刺补泻手法治疗疾病,提高诊病全局观,重观中轴,环顾四维,为临床针刺治病、防病提供新思路.

目的 观察康达心口服液对阳气亏虚血瘀型慢性心力衰竭患者心肺运动试验的影响.方法 收集阳气亏虚血瘀型慢性心力衰竭急性加重期患者65例,按随机数字表法分为对照组和康达心组,出院后病情稳定2～4周.对照组给予西药治疗,康达心组在对照组用药基础上加用康达心口服液治疗,两组均治疗12周,两组在纳入观察前1天及12周末分别进行明尼苏达心力衰竭生活质量评分(minnesota living with heart failure questionnaire,MLHFQ)、N末端B型利钠肽原(N teminal pro brain natriuretic peptide,NT-proBNP)测定以及心肺运动试验(cardiopulmonary exercise testing,CPET),记录峰值摄氧量(peak VO2)、无氧阈值(anaerobic threshold,AT)、峰值氧脉搏(VO2/HR)、二氧化碳通气当量最低值(VE/VCO2最低值)、二氧化碳通气当量斜率(VE/VCO2 slope)指标.结果 两组治疗前各观察指标无统计学差异(P>0.05);两组治疗后对比治疗前MLHFQ、NT-proBNP及CPET各参数均有显著改善,差异有统计学意义(P<0.05);治疗后康达心组较对照组MLHFQ、NT-proBNP下降,差异有统计学意义(P<0.05),peakVO2、AT、VO2/HR升高,VE/VCO2、VE/VCO2 slope下降,差异有统计学意义(P<0.05).结论 联合使用康达心口服液治疗阳气亏虚血瘀型慢性心力衰竭,能明显提高患者运动耐量、改善心肺功能,提高生活质量.

目的 探讨高血压中医证型与胰岛素抵抗及靶器官损害的相关性.方法 选择高血压(EH)患者150例,另选择体检健康者50例为对照组.两组研究对象均做以下检查:测定空腹血糖(FPG)、空腹胰岛素(FINS),计算胰岛素抵抗指数(HOMA-IR),测定靶器官损伤指标:肾损伤标志物尿微量白蛋白(mALB)、尿微白蛋白/尿肌酐(UACR),使用彩色多普勒超声测定颈动脉内-中膜厚度(IMT)、计算Crouse积分,测量左室舒张末期内径(LVDd)、舒张末期左室后壁厚度(PWT)、室间隔厚度(IVS),计算左室质量指数(LVMI).结果 EH组FINS、HOMA-IR、mALB、UACR、IMT、Crouse积分、LVMI均高于对照组(P<0.05);EH组HOMA-IR与mALB、UACR、IMT、Crouse积分、LVMI呈正相关(P<0.05).FINS及HOMA-IR痰湿壅盛组最高(P<0.05),肝火亢盛组最低(P<0.05),阴虚阳亢组及阴阳两虚组差别不显著(P>0.05).阴阳两虚组、痰湿壅盛组、阴虚阳亢组mALB、UACR高于肝火亢盛证组(P<0.05),阴阳两虚组、痰湿壅盛组组、阴虚阳亢组IMT、Crouse积分、LVMI显著高于肝火亢盛证组(P<0.01),阴阳两虚组LVMI高于阴虚阳亢组及痰湿壅盛组(P<0.05),余各组指标未见明显差异(P>0.05).结论 高血压痰湿壅盛证更易发生胰岛素抵抗,HOMA-IR及靶器官损害指标可作为中医辨证分型的客观量化参考指标.

目的:探讨盐酸曲美他嗪缓释片改善扩张型心肌病患者心功能的效果及用药安全性.方法:以2019年1月-2020年12月本院收治的104例扩张型心肌病患者为研究对象,随机分为两组.对照组52例,予以标准化心力衰竭治疗;试验组52例,在对照组治疗基础上加用盐酸曲美他嗪缓释片.治疗前后,对两组患者进行NYHA心功能分级评定、6 min步行试验,以及心脏超声测定左房内径(LAD)、左室舒张末期内径(LVEDd)与左室射血分数(LVEF),评价两组心功能改善情况.另外统计两组用药相关不良反应,评价安全性.结果:试验组心力衰竭治疗总有效率(90.38％)高于对照组(71.15％),试验组治疗后6 min步行距离(480.33±37.65)m,LVEF(44.06±5.04)％,均高于对照组,LAD(45.06±7.31)mm,LVEDd(65.82±8.56)mm,低于对照组,差异有统计学意义(P<0.05).试验组不良反应率(3.85％)与对照组(0)比较,差异无统计学意义(P>0.05).结论:规范化心力衰竭治疗基础上加用盐酸曲美他嗪缓释片治疗扩张型心肌病可以更好改善患者心功能,且不明显增加不良反应.

目的 探讨分析AMI患者应用冠状动脉内弹丸式注射水化肝素钠在PCI术中无复流的效果.方法 选取2015年1月至2020年1月我院收治的100例急性心肌梗死(AMI)并经皮冠状动脉介入术中出现无复流患者进行临床研究.按照随机数字表法将所有患者分为对照组(n=50)和观察组(n=50),对照组和观察组的治疗手段分别为硝普钠和水化肝素,对比观察两组患者.结果 将两组患者用药前后的心肌梗死溶栓治疗血流分级进行对比,经统计学计算得出,两组数值在用药前差异无统计学意义(P＞0.05),两组数值在用药后差异有统计学意义(P＜0.05),且单组用药后比治疗前差异有统计学意义(P＜0.05);将两组患者用药前后的计算矫正的TIMI帧数进行对比,经统计学计算得出,两组数值在用药前差异无统计学意义(P＞0.05),两组数值在用药后差异有统计学意义(P＜0.05),且单组用药后比治疗前差异有统计学意义(P＜0.05);将两组患者用药前后的心功能指标(LVEF、NT-proBNP、SV)进行对比,经统计学计算得出,两组数值在用药前差异无统计学意义(P＞0.05),两组数值在用药后差异有统计学意义(P＜0.05),且单组用药后比治疗前差异有统计学意义(P＜0.05);对照组和观察组的不良事件发生率分别为16％、4％,经统计学计算得出,两组数值对比差异有统计学意义(P＜0.05).结论 急性心肌梗死患者应用冠状动脉内弹丸式注射水化肝素钠可以显著改善患者的PCI术中的无复流现象、改善患者的心功能及预后水平,对临床推广发展具有现实意义.

目的:观察双清消斑饮对痰瘀互结型稳定性心绞痛患者心肺运动试验结果及生活质量的影响.方法:收集2020年1-6月在福建中医药大学附属人民医院就诊的痰瘀互结型稳定性心绞痛患者70例,按随机数字表法分为对照组和治疗组,各35例.对照组参照2018年《稳定性冠心病诊断与治疗指南》推荐的药物治疗方案治疗,治疗组在对照组基础上加用双清消斑饮治疗,两组观察期均为3个月,两组患者治疗前后分别进行心肺运动试验(cardiopulmonary exercise testing,CPET),记录峰值摄氧量(peak VO2)、无氧阈值(AT)、峰值氧脉搏(VO2/HR)、二氧化碳通气当量最低值(VE/VCO2最低值)、二氧化碳通气当量斜率(VE/VCO2斜率)等指标;采用SAQ、SF-36量表在治疗前后分别对两组患者进行调查.结果:两组治疗前心肺运动试验各观察指标比较差异无统计学意义(P>0.05);治疗后两组peak VO2、AT、VO2/HR均升高,且治疗组高于对照组(P<0.05);两组VE/VCO2最低值、VE/VCO2斜率均下降,且治疗组低于对照组(P<0.05);治疗组SAQ、SF-36评分高于对照组(P<0.05).结论:联合使用双清消斑饮能进一步改善痰瘀互结型稳定性心绞痛患者心肺功能及生活质量,治疗效果好.

目的 观察临方炮制三子养亲汤对哮喘的治疗效果,为其临床应用及推广提供一定的循证医学证据.方法 收集符合标准的哮喘病例60例,观察患者咳嗽、咯痰、喘促等临床症状及肺部体征,记录 β受体激动剂、茶碱、糖皮质激素使用情况.结果 3组哮喘患者治疗期间多索茶碱使用量比较无显著差异(F=0.365,P>0.05);三种方法治疗哮喘的疗效有差异(Z=8.268,P<0.05),进一步的两两分析发现,其中临方炮制组和对照组治疗哮喘的疗效无差异(P>0.05),而生品组与对照组和临方炮制组的疗效有差异(P<0.05);治疗前后比较,治疗后3组患者临床症状积分均较治疗前明显减少(P<0.01).结论 临方炮制三子养亲汤联合布地奈德福莫特罗粉吸入剂1吸和布地奈德福莫特罗粉吸入剂2吸治疗哮喘的效果相同,均优于生品三子养亲汤联合布地奈德福莫特罗粉吸入剂1吸.

Abstract Aim of the study: Salvianolic acid B(Sal B) as a natural compound extracted from Salvia miltiorrhiza, has been extensively used to protect cardiomyocytes from myocardial ischemia. Although Sal B has shown evident effects on cardiovascular diseases, the detailed mechanism is still unclear as yet. Herein, we intended to explorethe protective effects of Sal B on myocardial ischemic injury and the underlying mechanism. Methods and Results: Western blotting, immunofluorescence assay, flow cytometry and lentiviral transfection were performed. The mice with myocardial ischemic injury were intravenously given 10 mg/kg Sal B once daily for seven days, and then H9c2 cells were treated with Sal B (20, 40, 80 μmol/L). Sal B treatment protected cardiomyocytes from myocardial ischemia through relieving apoptosis. Transmission electron microscopy and fluorescence microscopy exhibited that Sal B significantly increased autophagic lysosomes and vacuoles in H9c2 cells. Administration with Sal B significantly up-regulated the expressions of autophagy-related factors such as LC3, Atg5 and Beclin 1 in H9c2 cells and myocardial tissues. The beneficial autophagic changes induced by Sal B were abrogated through pharmacological inhibition. Conclusions: This study provides a molecular mechanism by which Sal B potently inhibits apoptosis and oxidative stress upon myocardial ischemia by activating the AMPK-autophagy pathway. Sal B is a potential agent for treating myocardial ischemia.

目的 探讨康达心对异丙肾上腺素(ISO)诱导的心肌细胞凋亡及内质网应激的影响.方法 体外培养H9c2细胞,并将其分为对照组、ISO组和康达心组,采用流式细胞仪、蛋白印记法进行检测,比较各组H9c2细胞凋亡率及半胱氨酸蛋白酶(Caspase)-3、Caspase-9、葡萄糖调节蛋白78(GRP78)表达水平.结果 ISO组H9c2细胞凋亡率为36％,明显高于对照组的18％,差异有统计学意义(P<0.05).康达心组H9c2细胞凋亡率为25％,明显低于ISO组,差异有统计学意义(P<0.05).ISO组Caspase-3、Caspase-9及GRP78表达水平明显高于对照组和康达心组,差异均有统计学意义(P<0.05).结论 康达心通过抑制ISO诱导的内质网应激而有效抑制H9c2细胞凋亡,从而达到抑制氧化应激损伤的作用.