Telephium imperati (L) is a Caryophyllaceae species and is traditionally used in Morocco for its wound healing properties. In the present study the aim was to evaluate the wound healing activity of 5% ointment of hydroalcoholic extract of Telephium imperati (L.) on Wistar rat's skin Phytochemical screening and thin-layer chromatography were carried out to identify possible compounds. Eighteen male Wistar Albinos rats (150180 g) were divided into three groups. The test group treated with experimental ointment, control group treated with Vaseline and the reference group treated with standard drug (MADECASSOL (R)). Burn wounds were induced on dorsal area of the rats body. The wound area measurement was carried out every 5 days during 55 days; and also histopathology evaluation was performed 24 h after burn wound creation, 15th, 25th and 55th days. The results of physicochemical screening and thin-layer chromatography showed the presence of saponins, flavonoid and Quercetin. Significant wound healing activity was observed with topical application of Telephium imperati (L.) hydroalcoholic extract. Reduction of the wound area at the end of each treatment each was 95.5%, 97.5% and 75.75% for the test, reference and control groups, respectively. Moreover, the tissue histology of 5% ointment treated groups was most effect on the wound healing process with good fibroblasts proliferation, few inflammatory cells and well-organized collagen. The results of this study confirmed the traditional use of Telephium imperati (L) on wound healing management. (C) 2019 SAAB. Published by Elsevier B.V. All rights reserved.

Aim of the study: Immunohistochemical evaluation of WT1, E-cadherin, beta-catenin, EGFR and p53 on Tissue MicroArray (TMA) of 43 Moroccan benign, borderline and invasive epithelial ovarian tumours. Materials and methods: All 43 cases were collected from the pathology department of the Institut National d'Oncologie in Rabat, Morocco, and comprised 34 carcinomas, 4 borderline serous and mucinous tumours and 5 benign tumours. Patients were between 20 and 74 years old with a mean age of 50 years. TMAs and the IHC study were supported by a grant from the IAAE (International Agency of Atomic Energy) and prepared in the pathology department of Columbia University in New York. 3 cores were selected from each case, and the peroxydase-anti peroxydase technique was used for the study of the different markers (DAKO Cytovision, Carpinteria CA). Results: 23.25% of the cases (10/43) were WT1 positive and were serous tumours (including one poorly differentiated adenocarcinoma). 72% of the cases (31/43) showed reduced (19/43) or no (12/43) membranous expression of E-cadherin, and all the tumours showed reduced membranous expression with cytoplasmic expression (5/43) or no expression (38/43) of beta-catenin. p53 overexpression (13/43) was exclusively observed in 58% (11/19) of the serous carcinomas and 2/3 poorly to moderately differentiated adenocarcinomas, of which 9/13 were EGFR + and 6/13 were E-cadherin +. 70% of the cases (30/43) showed EGFR membrane staining, and 2 cases were not interpretable. Conclusion: TMA is a feasible tool to study a large number of cases allowing comparative analysis of the expression of different biomarkers. To our knowledge, this is the first study of 5 biomarkers to be done on TMAs from 43 moroccan benign, borderline and invasive epithelial ovarian tumour samples. This would allow for larger studies with the aim of analyzing the significance of these biological markers and their impact in clinical trials.

Les auteurs rapportent un cas de tumeur stromale de l'intestin grele diagnostique a l'Institut National d'Oncologie. Celui-ci est remarquable par sa multiplicite et son caractere dissemine au sein de la cavite abdominale. A travers une revue recente de la litterature, les auteurs soulignent les circonstances particulieres du diagnostic, les aspects morphologiques, immunohistochimiques, et les divers concepts histogenetiques de ces tumeurs. Les auteurs commentent enfin les difficultes d'etablissement d'un pronostic fiable pour ces tumeurs pour lesquelles aucun consensus n'a ete etabli jusqu'a ce jour.

La tuberculose occupe une place non negligeable dans les infections survenant au cours de certains cancers notamment les lymphomes. Dans certains cas le tableau clinique est evocateur d’une tuberculose, mais dans la majorite des cas il existe une symptomatologie non evocatrice. Les auteurs rapportent un cas de tuberculose associe un lymphome gastrique de Malt; ils mettent l’accent sur l’interet de la recherche d’une telle lesion difficilement accessible a la biopsie qui seule permet d’aboutir au diagnostic par le biais de l’examen anatomo-pathologique.

Le gliosarcome est une tumeur rare du systeme nerveux central a double composante neurogliale et mesenchymateuse. Cette entite suscite des discussions quant a ses caracteristiques histopathologiques, son histogenese et la conduite a tenir. Les auteurs rapportent le cas d’un patient age de 57 ans ayant presente une hypertension intra-cranienne durant 3 mois. L’Imagerie par Resonance Magnetique (IRM) et le scanner avaient mis en evidence la presence d’un processus tumoral frontal expansif entoure d’oedeme. Le diagnostic de meningiome a ete evoque a l’examen histopathologique d’une biopsie stereotaxique de la tumeur. Le patient est decede une semaine apres l’intervention chirurgicale dans un etat comateux.

In situ hybridization is a molecular technique which allows for in situ detection, on paraffin sections of tumoral specimens, of viral nucleic acid using complementary probes hybridizing with the viral genetic material present in the infected cells. This technique, which has been introduced for the first time in our country, is highly interesting as pathogenic, diagnostic and epidemiological tools detecting, without the need for RNA extraction, the causative agent in some diseases. This is indeed the case in some viral-induced cancers as the rhinopharynx one which is closely linked to the presence of Epstein-barr virus.

Pseudomyxoma peritonei is a rare pathology, which poses number of questions about its physiopathology. We report two cases of appendiceal mucocele and mucinous ovarian tumour with pseudomyxoma peritonei. We discusse the morphological aspect of this affection. A mucinous metaplasia of mesothelial cells caused by local diffusion of the mucus is proposed in order to give an explauration to this microscopically benign affection but very aggressive due to its numerous recurrences.

Le rhabdomyosarcome est une tumeur mésenchymateuse rare. La localisation paratesticulaire est la plus fréquente des localisations urogénitales. C’est une tumeur qui intéresse surtout l’enfant et reste exceptionnelle chez l’adulte. Le pronostic est mauvais, et le traitement repose sur le trépied chirurgie, chimiothérapie et radiothérapie. À la lumière d’une revue de la littérature, nous discutons les modalités diagnostiques et thérapeutiques de cette tumeur.

La malakoplakie est une maladie granulomateuse benigne rare. La theorie physiopathologique la plus soutenue est l’origine infectieuse associee a une atteinte de la fonction des macrophages. Son aspect clinique ou paraclinique n’a pas de particularites, contrairement a sa specificite histologique caracterisee par la presence de cellules de Von Hansemann. La forme pseudo-tumorale et la localisation tubo-ovarienne sont rares. Le traitement est surtout medical associant antibiotiques et cholinergiques, l’exerese chirurgicale n’est indiquee que dans les formes pseudo-tumorales ou en cas de destruction totale de l’organe atteint. Nous rapportons un cas de malakoplakie tubo-ovarienne pseudo-tumorale simulant un cancer de l’ovaire et nous discutons les connaissances actuelles sur cette maladie rare et son approche therapeutique.

Introduction : Malignen change in dermatofibrosarcoma protuberans is a rare phenomenon. We present one case. Case report : a patient operated for local recurrences of dermatofibrosarcoma. The histopathological study showed a fascicular and highly cellular histological pattern in the dermis, with importante mitotic activity.Sometimes the nucleus was central chow small indentations produced by multiple small vacuoles like a lipoblast, with an extensive capillary network apparence the diagnosis of liposarcoma was proposed. Discussion : malignen change of dermatofibrosarcoma is defined by its herringbone architecture, hypercellularity, and increased mitotic rate .Occasionally, can lead to misdiagnosis with other spindle-cell tumors especially liposarcoma which has a worse prognosis. Multiple sampling and immunohistochemistry with antibody CD34 help to establish the right diagnosis. Conclusion: The degree of aggression is related to the histological grade and of sarc omatous transformation. The prognosis of the transformed dermatofibrosarcoma is more aggressive than the classic than one in liposarcoma.

Peripheral primitive neuroectodermal tumors (PNET) are rare malignant tumors, affecting mostly children and adolescents and have been described in breast in eight case reports only. In this paper, we present a case of bilateral mammary ES/PNET where distinction between primary and metastatic diseases was discussed through a literature review. The aim of this work is to demonstrate that although rare, the possibility of PNET should be kept in mind while evaluating a palpable breast abnormality in a young female.

Langerhans' cell histiocytosis is a non-malignant proliferative disease of unknown etiology. It is a rare illness affecting mainly children and young adults with a male predominance. It can affect one or many organs. The bone locations are the most frequent. We present one case of multifocal Langerhans' cell histiocytosis in a 22-year-old male patient followed for diabetes insipidus with an enlargement of the pituitary stalk at the magnetic resonance imaging (MRI), and a lytic bone lesion revealed in lumbar spine (L2) by Tc-99m-HMDP scintigraphy conducted in planar mode, and then in Single Photon Emission Computed Tomography coupled to Computed Tomography (SPECT/CT) in hybrid mode. To this end, we propose to highlight diagnostic gain of SPECT/CT compared with SPECT and planar scintigraphy to characterize radiotracer uptake abnormalities in bone in Langerhans' cell histiocytosis. (C) 2012 Elsevier Masson SAS. All rights reserved.

M. El Mzibri1,*, M. Attaleb1, R. Ameziane El Hassani1, M. Khyatti2, L. Benbacer1, M. M. Ennaji3 and M. Amrani4 1Unite de Biologie et Recherche Medicale, Centre National de l'Energie, des Sciences et Techniques Nucleaires (CNESTEN), Rabat 2Laboratoire d’Onco-Virologie, Institut Pasteur du Maroc, Casablanca 3Laboratoire Virologie Hygiene and Microbiologie, Faculte des Sciences et Techniques Mohammedia 4Service d’Anatomie Pathologique, Institut National d’Oncologie, Rabat Morocco