目的 探究S100B抑制剂Pentamidine对内侧颞叶癫痫(MTLE)不同时期COX-2、IL-1β蛋白表达的影响及其可能的作用机制.方法 160只小鼠随机分为对照组和模型组,模型组小鼠右背海马区给予注射50 nL海人酸-PBS溶液(1μg/μL),对照组小鼠则注射等体积生理盐水.分别于建模前1 d和建模6 h后向各组小鼠尾静脉注射Pentamidine(5mg/kg)或等体积的PBS,每天1次,连续7 d.MTLE成功诱导后12 h(急性期)、7 d(潜伏期)、40 d(慢性期)取小鼠海马组织行后续实验.采用RT-PCR检测小鼠海马组织S100B mRNA表达水平;采用尼氏染色检测小鼠海马组织尼氏小体和神经元丢失;采用Western blot检测小鼠海马组织COX-2和IL-1β蛋白表达.结果 与对照-PBS组比较,急性期、潜伏期和慢性期-PBS组海马组织中S100B mRNA表达不同程度增加(P<0.05);与各PBS组比较,不同时期Pentamidine组S100B mRNA的表达下降(P<0.05).尼氏染色显示,与对照-PBS组比较,急性期、潜伏期和慢性期-PBS组神经元结构不同程度受损,尼氏小体数量下降(P<0.05);与各PBS组比较,不同时期Pentamidine组的神经元受损程度减轻,尼氏小体数量明显增加(P<0.05).Western blot显示,与对照-PBS组比较,急性期、潜伏期和慢性期-PBS组海马组织COX-2、IL-1β和phospho-NF-κB的蛋白表达增加(P<0.05);与各PBS-组比较,不同时期Pentamidine组COX-2、IL-1β和phospho-NF-κB蛋白表达下调(P<0.05).结论 S100B抑制剂Pentamidine可通过下调COX-2和IL-1β的表达降低MTLE小鼠海马炎症反应和神经损伤,为MTLE的临床治疗提供重要的参考价值.

目的 探究内侧颞叶癫痫(MTLE)不同时期S100B、RAGE和COX-2的动态表达变化及其临床意义.方法 120只SPF级雄性C57BL/6小鼠随机分为对照组和模型组,模型组小鼠右背海马注射50nl海人酸(KA,1μg·μL-1),对照组小鼠则注射等体积的PBS溶液.分别于癫痫诱发成功后6h(急性期)、7d(潜伏期)、40d(慢性期)取各组鼠脑行后续实验.采用免疫组化检测个各组小鼠不同时期海马组织中RAGE和S100B的表达水平;采用Western blot检测各组小鼠不同时期海马组织中COX-2的表达水平.结果 对照组小鼠未见任何癫痫发作表现.模型组小鼠KA注射1h后出现面部肌肉抽搐并逐渐加重至全身痉挛,脑电图(EEG)监测到明显的癫痫样放电;7d后癫痫发作基本消失,进入潜伏期;30d后小鼠开始出现慢性、反复性癫痫发作.免疫组化显示,急性期小鼠海马组织中RAGE和S100B蛋白表达较对照组显著增加(P<0.05);与急性期比较,潜伏期和慢性期小鼠RAGE和S100B表达逐渐降低,差异有统计学意义(P<0.05).Western blot结果显示,急性期小鼠海马组织中COX-2的蛋白表达较对照组显著增加(P<0.05);潜伏期和慢性期小鼠海马组织中COX-2的表达较急性期显著下降,差异有统计学意义(P<0.05).结论 RAGE、S100B和COX-2的表达在MTLE小鼠急性期、慢性期和潜伏期显著增加,有望为MTLE的临床治疗提供新的靶点.

血红素加氧酶-1(heme oxygenase-1,HO-1)是一种内源性抗氧化酶,具有抗氧化、抗炎、抗凋亡、促血管生成、细胞保护的作用[1-2].今将HO-1与帕金森病(parkinson's disease,PD)关系的研究进展进行综述.

多系统萎缩(multiple system atrophy,MSA)是一组散发性、进行性、成年期发病的神经系统变性疾病,相继累及锥体外系、锥体系、小脑和自主神经系统等多个部位.临床主要表现为小脑共济失调、帕金森病(Parkinson's disease,PD)、自主神经功能障碍及锥体束症状的不同组合.根据Gilman 等[1]在2008年修订的诊断标准,MSA患者可分为小脑共济失调为主(MSA cerebellar variant,MSA-C)型和PD综合征为主(MSA parkinsonian variant,MSA-P)型2个亚型.PD是一种常见的神经退行性疾病,以运动不能、肌强直、静止性震颤及姿势反射障碍为特征性表现.多系统萎缩临床表现较为复杂,尤其疾病的早期很难与PD相鉴别,今将多系统萎缩与PD的鉴别要点作一综述.

精神障碍是帕金森病常见的非运动症状之一,以抑郁和焦虑表现为主,对患者情绪稳定和生活产生极大的破坏,却很少被临床工作者正确诊断,更难做到行之有效的治疗,致使患者的生活质量下降.为了更好地了解帕金森病伴发精神障碍的发病机制及临床干预措施,本文将从多巴胺、5-羟色胺、去甲肾上腺素等神经递质及心理因素等方面综述如下.

Parkinson's disease (PD) is usually characterized by motor symptoms including bradykinesia,rigidity,tremor,and postural instability.Rapid eye movement (REM) sleep behavior disorder (RBD) is characterized by lack of muscle atonia during REM sleep and enactment of dream content.RBD is closely associated with PD.The pathophysiological mechanisms of PD with RBD may be related to defects of nigrostriatal dopamine and locus coeruleus/sub-locus coeruleus complex in brainstem.RBD has high incidence in PD patients,mainly presented with more severe non-motor symptoms,and more prominent life ability and life quality damage.Injury prevention should be given to PD patients with RBD besides positive intervention of PD.We mainly discussed the above contents in this paper.

Objective To investigate the relationship of RS3831458 polymorphism of α-synuclein gene and Parkinson’s disease ( PD) in different Hoehn-Yahr ( H-Y) grading.Methods The RS3831458 polymorphism of α-synuclein were detected by gene sequencing in 95 PD patients ( PD group) and 95 healthy controls ( normal control group) .The relationship between RS3831458 polymorphism and PD were analyzed.Results Compared with normal control group, the frequency of -/CT genotype in PD group was significantly higher, and the frequency of AG/CT genotype was significantly lower ( all P<0.05 ) .Compared with H-Y <3 PD subgroup, the frequency of -/CT genotype in H-Y≥3 PD subgroup was significantly higher, and the frequency of AG/CT genotype was significantly lower(all P<0.05).The-/CT genotype was positively correlated with the disease severity of PD (χ2 =4.633, OR=2.456, 95%CI:1.076-5.605, P<0.05), and AG/CT genotype was negatively correlated with the disease severity of PD (χ2 =4.633, OR=0.407, 95%CI:0.178-0.929, P<0.05).Conclusions The frameshift mutation of RS3831458-/CT genotype can exacerbate the PD.

Objective To investigate the association of α-synaptophysin A53T gene polymorphism with parkinson's disease(PD) in Chinese people.Methods The conventional polymerase chain reaction was used to detect the α-synaptophysin A53T gene polymorphism in 224 sporadic PD patients(PD group)and 154 healthy individuals(control group).According to the Hoehn-Yahr(H-Y) classification standard, PD patients were divided into H-Y ≥ 3 group(n=172) and H-Y ≤ 2.5 group(n=52).Each genotype and alleles frequencies as well as the A53T gene expression and their relation to the severity of parkinson's disease were analyzed with Chi-square test of SPSS19.0.Results The frequency of the A53T genotype of A/A were 40(17.9％) and 10(6.5％) (x2 =10.267, P=0.001, OR=3.13,95％ CI =1.514-6.473) in the PD group and control group, respectively.The frequency of the allele A was 160(35.7％) and 70(22.7％) (x2 =14.543, P=0.000, OR=1.889,95％ CI =1.359-2.625) in the PD group and control group,respectively.The frequency of the A53T genotype of A/A was 30(17.4％) and l0 (19.2％) in the H-Y ≥ 3 group and H-Y ≤ 2.5 group, respectively, with significant difference (P=0.003,0.007) as compared to the control group.The frequency of the allele A was 122(35.5％) and 38(36.5％) in the the H-Y ≥ 3 group and H-Y ≤ 2.5 group,respectively, with significant difference (P=0.000,0.006) as compared to the control group.There was no statistically significant difference of the A53T genotype of A/A and the allele A between the H-Y≥3 group and H-Y≤2.5 group (P=0.768,0.841).Conclusion The A53T gene polymorphism is the risk factor of Parkinson's disease in Chinese people, but it isn't correlated to stage of sporadic parkinson' s disease.

LNG在储罐内长时间存储过程中,受外部热源的侵扰会发生蒸发,罐内压力升高,安全存储时间缩短并可能导致其分层翻滚,乃至LNG大量急剧蒸发,不仅浪费资源且造成安全隐患.建立密闭LNG储罐内静态蒸发模型,对初始充满率、储罐容积、环境温度、罐壁导热系数、LNG含氮量等影响因素进行研究,结论如下:其一,在同一初始充满率下,在储罐最大工作压力范围内,罐内压力随安全存储时间呈正比例关系增长.其二,在储罐最大工作压力范围内存在最优充满率,在最优充满率时储罐有最大的安全存储时间;当初始充满率小于最优充满率时,安全存储时间随初始充满率的减小而减小;当初始充满率大于最优充满率时,安全存储时间随初始充满率的增大而减小.其三,储罐的尺寸越小,储罐所具有的最大承压能力越大,最优充满率越大,安全存储时间越长.其四,外界环境温度越高以及罐壁导热系数越大,罐内压力随时间增长率越大,储罐的安全存储时间越短.其五,LNG组分中含氮量越高,罐内压力随时间增长率越大,储罐的安全存储时间越短.

帕金森氏病(PD)是一种中枢神经系统变性疾病，临床特征主要以运动障碍为主，还包括一些非运动症状(认知下降、记忆力减退、情绪变化、睡眠模式异常、味嗅觉丧失、多汗等)。发病主要原因是纹状体内多巴胺能神经元病理性衰退，新的证据表明，此种衰退病程与病理性神经重塑性超敏多巴胺受体信号传导增强有一定相关，甲状腺激素水平异常(主要是三肽促甲状腺激素释放激素TRH异常)可介导此调控机制。

Objective To explore the effect of the boiling disinfection in district hospitals. Methods Two hundred and forty pieces of outpatient surgery equipment were divided into group A and B, the patients in group A were soaked in chlorine disinfectant (containing chlorine 500 mg/L) for 30 minutes after regular hand-washing, while the patients in group B were boiled in 90℃ for 5 minutes after regular hand-washing. Bacterial culture was executed every two days, and corrosion and scissors sharpness were examined after one month. Results Bacterial culture results in both groups were up to the standard after two groups of surgical instruments disinfection; The rust rate and sharpness in group B were better than group A, the results had a significant difference (P < 0.05). Conclusion Boiling disinfection can meet the standard of disinfection and it is efficient in district hospitals when disinfection machines are not available. It can be achieved the high work quality, efficiency, and reduce material consumptions. It can also reduce consumption, pollution as well as the risk of occupational exposure.

Objective To analyze and investigate the risk factors of nursing in department of internal medicine, and put forward corresponding management countermeasures aimed at the risk problem. Methods Research object was 68 cases of patients in department of internal medicine from March 2011 to March 2012 in a hospital. The nursing risk factors of the 68 patients were analyzed and investigated retrospectively. Results All patients received scientific, effective nursing. However, 1 case occurred transfusion leakage problems, and 2 patients had economic disputes with the hospital. The overall rate of complaints was 2.94%. Conclusion According to the hospital's department of internal medicine, there may be a number of nursing risk should adopt corresponding risk management measures, so as to reduce a risk as far as possible events, help the patients recover, and also conducive to the harmonious relationship between doctors and patients.

Objective To explore the relationship between FABP2 54 gene polymorphism and serum lipid levels in different ethnic patients with coronary heart disease.Methods We collected fasting blood from participants,then extracted DNA from whole blood,and detected lipids from serum.We performed 54A/T FABP2 genotyping for participants in the case and the control groups by polymerase chain reaction (PCR) and DNA restriction enzyme digestion techniques.Results ( 1 ) In Hans population with coronary heart disease,the allelic frequency of point in 54T gene was 0.542,and 54A gene was 0.458 ; in urban Mongolian with coronary heart disease,the allelic frequency of point in 54T gene was 0.708,and that of 54A gene was 0.292.The allelic frequency of point in 54T and 54A genes were 0.284 and 0.716 respectively in the Han population control group and 0.353 and 0.647 respectively in the normal urban Mongolian group.Compared with the control groups,mutant 54T allele frequency in the case groups of Han and urban Mongolian was significantly higher ( Han:x2 =14.967,P ＜ 0.05 ; Mongolian:x2 =28.083,P ＜ 0.05 ).Compared with the Han Chinese population with coronary heart disease,the mutant 54T allele frequency in urban Mongolian with coronary heart disease was significantly (x2 =7.111,P ＜ 0.05 ).There was no significant difference in mutant 54T allele frequency between the Mongolian control group and the Han control group ( x2 =3.392,P ＞ 0.05 ).( 2 ) Patients with coronary heart disease who carry Thr54 ( + ) had a significantly higher level of fasting plasma TG [ CC Thr54 (-):( 1.89 ± 0.57 ) mmol/L,CC Thr54 ( + ):( 3.92 ± 1.63 ) mmol/L; CM Thr54 (-):(2.23 ± 0.13 ) mmol/L,CM Thr54 ( + ):(4.03 ± 1.14) mmol/L; Hans:P =0.001,Mongolian:P =0.035 ],and LDL-C [ CC Thr54 (-):( 3.09 ± 0.92) mmol/L,CC Thr54 ( + ):(4.05 ± 1.14 ) mmol/L ; CM Thr54 (-):( 4.26 ± 0.08 ) mmol/L,CM Thr54 ( + ):( 5.10 ± 0.56 ) mmol/L; Hans:P =0.025,Mongolian:P =0.045 ] than those with Thr54 (-).Conclusion (1) Threre is FABP2 gene polymorphism in urban Mongolian and Han population.Gene mutation frequency is in accordance with Hardy-Weinberg law of genetic equilibrium,suggesting the sample group representative.(2) FABP2 polymorphism may have a certain contribution to dyslipidemia in patients with coronary heart disease.FABP2 gene polymorphism may be associated with coronary heart disease.Individuals of Mongolian with FABP2 Thr54( + ) has a higher risk of coronary heart disease.

Objective To explore the clinical diagnositic significance of the interleukin-6 (IL-6), amyloid protein Aβ1-40 (Aβ1-40), macrophage clony stimulating factor (M-CSF), interleukin-1β(IL-1β) and tumor necrosis factor-α(TNF-α) in plasma in Alzheimer's disease (AD). Methods Seventy four AD patients were chosen, and 100 healthy elderly were selected as the control. ELISA test methods were used to test the level of IL-6, Aβ1-40, M-CSF , IL-1β and TNF-αin peripheral blood. Results Plasma Aβ 1-40 concentration in AD patients of (108.4±6.5) pg/mL had no significant difference compared with the control group of (103.5±7.1) pg/mL (P 0.05). The concentration of IL-6, Aβ1-40, M-CSF , IL-1β,TNF-α in AD patients all had statistical significance compared the controls (P 0.01), with the comparison of (180.9±50.6) pg/mL vs (75.2±45.1) pg/mL, (524.6±68.1 ) pg/mL vs (320.5±80.8) pg/mL, (40.45±6.14) pg/mL vs (30.64±7.50) pg/mL,(60.52±5.64) pg/mL vs (36.64±15.32) pg/mL. Conclusion The test of Aβ1-40 level in plasma may be meaningless for the diagnosis of AD, the test of IL-6, M-CSF, IL-1β, TNF-α level may be meaningful for clinical diagnosis as a biological indicator for AD.

Objective To investigate relationship between Ala 54 Thr polymorphism in the intestinal fatty acid-binding protein (I-FABP) and plasma lipids in old people.Methods We collected fasting blood samples of selected groups,then we extracted DNA from whole blood,and detected lipids from serum.The 54A/T I-FABP genotypes were analyzed in 72 Han Chinese elderly by polymerase chain reaction (PCR) and DNA restriction enzyming.Total cholesterol (TC),triglyceride (TG),high density lipoprotein (HDL-C) and low-density lipoprotein (LDL-C) were detected.Genotype subgroups were Thr54 (-) and Thr54 (+) groups (36 cases for each).Results Compared with Thr54 (-) genotype group,Thr54 (+) genotype group had higher levels of plasma TC[(4.50±0.73) mmol/L vs.(5.48±0.49)mmol/L],TG[(1.08±0.48) mmol/L vs.(2.02±0.53)mmol/L],LDL-C [(3.10±0.44) mmol/L vs.(3.50±0.66)mmol/L],and had lower level of HDL-C [(1.14±0.25) mmol/L vs.(0.96±0.23)mmol/L](t=-6.67,-7.84,-3.03,3.05,all P＜0.05).

Heart-type fatty acid-binding protein is a soluble cytoplasmic protein,and expressed in the brain cells.The levels of heart-type fatty acid-binding protein were significantly increased in serum in the early stage of cerebral infarction,While pati-ents were suffering from acute cerebral infarction.it is more sensitive than conventional neuro-biochemical markers,named neuron specific enolase(NSE) and S100B.The concentrations of serum were associated with the areas and injured neurological functions.So it is potential to be a new type of neuro-biochemical markers for the early diagnosis of cerebral infarction.

<正>阿尔茨海默病是慢性进行性中枢神经系统变性病导致的痴呆,主要临床表现是渐进性远近记忆力障碍、分析判断能力衰退、认知障碍、人格改变和行为失常,进而意识模糊,最后多死于肺炎和尿路感染。是导致老年人痴呆最常见的疾病,严重影响老年人的生命质量,这一疾病可以持续20余年,给个人、家庭和社带来沉重的负担和痛苦。随着人类寿命的延长和人口老龄化AD的发病率也将不断增加。目前Alzheimer病(Alzheimer disease,AD)临床诊断尚无明确的特异性生化指标,

Serum uric acid is the important outcome of purlne metabolize.Recent reports show that hyperuricemia plays an important role in the occurrence and development of coronary heart disease.Endothelin is a short-chain peptides constituted by 21 amino acid residue,with a strong vasoconstriction,which involved a of physical activities and the disease process,and is becoming a hot topic in the cardiovascular system.This review includes some progesses about relationship between hyperuricemia and Endothelin.

The geological reserves of south Gudao Oilfield were 2271.6×104t,it's reservoir characters were multilayer,thin reservoir,fault zones and poor reservoirs,which determined that it was difficult for oilfield management and development.At present,the recovery percent was only 5.3%,recovery ratio was 11.3%,it had a good foundation for tapping the potential.Fine geological research was conducted on Layers Ng5-6 and Ng1+2～4for implementing supporting adjustment techniques,for the highly controlled well pattern of middle Ng5-6 heavy oil layer,a fine evaluation was performed on the heterogeneity of reservoirs and fluids to implement sub-zoning and low efficient waterflooding transferred to thermal recovery,for Ng1+2～4 heavy-oil layer with poor well pattern control and complex structure,structural and reverse reservoir interpretations were carried out to clarify the resources and adjust the strata development for the purpose of raising the oil porduction level of reserves.Improving the development effect and enhancing oil recovery and for effective development of the south Gudao Oilfield.

Objective:To investigate the genetic association between PvuⅡpolymorphisms of estrogen receptor 1(ESR1) and migraine.Methods:A case-control study was performed in 41 migraineurs and 44 controls.For the subjects,ESR1 PvuⅡgenotypes were detected by polymerase chain reaction and restriction fragment length polymorphisms assay.Results:In the migraineurs,PP frequency was 17.1%, Pp frequency was 43.9%and pp frequency was 39%,respectively;in the controls,the corresponding frequency was 13.6%,38.6%and 47.7%,respectively.There was no significant difference in the frequency of ESR1 polymorphisms between the migraineurs and the contros.(χ~2=0.676,P=0.713). Conclusions:The PvuⅡpolymorphism in ESR1 might have no significant correlation with migraine.