目的:探讨光动力联合CO2激光治疗对尖锐湿疣患者外周血T淋巴细胞亚群及炎性因子的影响.方法:收集在我院接受治疗的尖锐湿疣患者临床资料,基于特定时间段内(2020年2月-2021年2月)对其进行选取(80例),将所有选取患者的病例信息均输入Excel系统,按照系统编号的单双数分为2组(各40例),以SPSS23.0为统计工具,其中40例双数者联合光动力治疗,将其设为A组,另40例单数者选择CO2激光治疗,将其设为B组,就A组、B组治疗效果展开对比.结果:A组临床疗效(95.00％)显著高于B组(80.00％)(P<0.05);治疗后,A组CD3+、CD4+、CD4+/CD8+显著高于B组(P<0.05),CD8+显著低于B组(P<0.05);治疗后,A组炎性因子水平显著低于B组(P<0.05);不良反应:A组(7.50％)显著低于B组(25.00％)(P<0.05).结论:该研究具有可行性.

目的:观察蒿秦化斑方联合雷公藤多苷对皮炎湿疹患者免疫功能的影响.方法:选取168例皮炎湿疹患者作为研究对象,按随机数字表法分为治疗组及对照组各84例,对照组使用雷公藤多苷治疗,治疗组使用蒿秦化斑方联合雷公藤多苷治疗.比较2组临床疗效,比较2组治疗前后白细胞介素-4(IL-4)、白细胞介素-10(IL-10)、γ干扰素(IFN-γ)及T细胞亚群TH1、TH2、TH1/TH2的变化,比较2组治疗前后皮肤屏障功能及中医证候积分的变化.结果:治疗后,2组IL-4、IL-10、IFN-y水平均较治疗前下降,治疗组IL-4、IL-10、IFN-γ水平均低于对照组,差异均有统计学意义(P＜0.05).治疗后,TH1、TH2水平均较治疗前下降,TH1/TH2水平均较治疗前上升,差异均有统计学意义(P＜0.05);治疗组TH1、TH2水平均低于对照组,TH1/TH2水平高于对照组,差异均有统计学意义(P＜0.05).治疗后,2组经表皮水份流失水平均较治疗前下降,角质层含水量、皮脂含量均较治疗前上升,差异均有统计学意义(P＜0.05);治疗组经表皮水份流失水平低于对照组,角质层含水量、皮脂含量均高于对照组,差异均有统计学意义(P＜0.05).治疗后,2组中医证候积分均较治疗前下降,治疗组中医证候积分低于对照组,差异均有统计学意义(P＜0.05).治疗组临床疗效总有效率为97.62％,对照组临床疗效总有效率为89.29％,2组比较,差异有统计学意义(P＜0.05).结论:蒿秦化斑方联合雷公藤多苷治疗皮炎湿疹疗效较好,可缓解患者临床症状,改善炎症因子水平及免疫功能,提高患者皮肤屏障功能.

目的:探讨加巴喷丁、多塞平联合治疗方案应用于带状疱疹神经痛治疗中的效果.方法:共纳入贵阳市第二人民医院收治的86例带状疱疹神经痛患者(2020年1月至2021年6月入院)作为研究对象,以随机分组方式分为参照组和治疗组(各43例),两组均接受常规抗病毒治疗,在此基础上参照组行加巴喷丁治疗,治疗组在以上治疗基础上联合多塞平,比较治疗效果.结果:较参照组79.07％,治疗组治疗有效率95.35％更高(P＜0.05).治疗前,两组患者疼痛评分、血清疼痛介质水平、血清炎性因子水平相似(P＞0.05).治疗后,较参照

水痘-带状疱疹病毒（varicella zoster virus）初次感染人体引起水痘,再次感染后将体内的病毒再次激活引起带状疱疹（HZ）,临床主要表现为皮肤的疱疹病变,同时伴有明显的神经痛。目前对带状病毒的治疗主要采取足量、足疗程的抗病毒及营养神经药物,但部分患者即使皮损消退也会遗留不同程度的神经痛（PHN）,影响患者的生活质量。目前,临床研究认为带状疱疹神经痛主要是由于病毒侵犯

目的 探讨生殖器癌患者人类乳头状瘤病毒感染及免疫调节状态的变化情况.方法 将105例生殖器癌患者设为观察组,105名健康人员设为对照组,检测并比较两组人类乳头状瘤病毒感染情况、细胞免疫及红细胞免疫指标,并对不同疾病分类生殖器癌及TNM分期患者的检测结果进行分析.结果 观察组高危型人类乳头状瘤病毒感染率显著高于对照组(P<0.05),细胞免疫及红细胞免疫指标检测结果均显著差于对照组(P<0.05).宫颈癌患者高危型人类乳头状瘤病毒感染率显著高于其他生殖器癌患者(P<0.05),但不同疾病分类生殖器癌患者的细胞免疫及红细胞免疫指标检测结果比较差异无统计学意义(P>0.05);较高TNM分期患者的高危型人类乳头状瘤病毒感染率显著高于较低分期者(P<0.05),细胞免疫及红细胞免疫指标检测结果显著差于较低分期者(P<0.05).结论 生殖器癌患者高危型人类乳头状瘤病毒感染率较高,且机体的免疫调节状态相关指标相对较差,应给予充分的重视及干预.

Leprosy is a chronic infectious diseasecaused by Myc-obacterium leprae. Majorviolations of human skin and nerves, if not treatment can cause permanent damage to the skin, nerves, etc. At present, Chinese treatment of leprosy prevention, and for those patients who have been suffering from leprosy, treatment is also worth studying deeper exploration. The research method of this article is through the T cellreceptor gene drift a chain V region each family CDR3 immune pedigree analysis technique to monitor leprosy patient TCR a chain CDR3 pedigree, and preliminary analysis of CDR3 PCR products and TRAVcloning of quantitative hyperplasia of the family, in order to provide a new research direction for T cell immune response changes after the patients infected with leprosy.

目的：比较苄星青霉素与阿奇霉素治疗妊娠期梅毒患者临床疗效及对新生儿的影响。方法选取妊娠期梅毒患者62例，随机分为苄星青霉素组和阿奇霉素组，各31例，对比2组疗效及其对新生儿的影响。结果治疗后，苄星青霉素组有效26例(83.87%)，无效5例(16.13%)；阿奇霉素组有效27例(87.10%)、无效4例(12.90%)，组间有效率比较差异无统计学意义。对2组产妇的焦虑情绪等进行比较，观察组的SAS评分为（14.5±2.7）分，对照组为（23.5±4.7）分，观察组产妇优于对照组，差异具有统计学意义（P＜0.05）；对2组新生儿的Apgar评分进行比较，观察组为（11.31±1.34）分，对照组为（22.43±3.06），观察组观察组优于对照组，差异具有统计学意义（P＜0.05）。结论苄星青霉素与阿奇霉素治疗妊娠期梅毒患者临床疗效及对新生儿的影响相当，均可作为治疗妊娠期梅毒行之有效的方法。

目的：观察在带状疱疹患者中使用泛昔洛韦联合胸腺肽治疗的效果。方法：选取患有带状疱疹的患者一共100例，将这些患者平均分为两组，观察组使用泛昔洛韦联合胸腺肽进行治疗，对照组患者仅使用泛昔洛韦进行治疗，观察两组患者的治疗效果。结果：使用泛昔洛韦联合胸腺肽进行治疗的患者，治疗的有效率明显高于仅使用泛昔洛韦进行治疗的患者，并且两组具有明显的差异（P<0.05）。结论：对患有带状疱疹的患者使用泛昔洛韦联合胸腺肽进行治疗具有很好的治疗效果，同时不良反应较少，因此具有临床推广的价值。

Objective: To observe clinical effects of the Hanlian capsule plus NB-UVB on vitiligo. Methods: 112 patients were randomly divided into three groups, 37 patients in the group A were given the Hanlian capsule; 37 patients in the group B were given NB-UVB; 38 patients in the group C were given the Hanlian capsule plus NB-UVB. Results: In the group A, 3 cases were improved, 8 cases were improved better, the total efficacy was 59.46%; in the group B, 4 cases were improved, 8 cases were improved better, the total efficacy was 64.86%; in the group C, 6 cases were improved, 15 cases were improved better, the total efficacy was 76.32%; the difference between the group C and the other groups was statistically significant (P<0.05). Conclusion: The Hanlian capsule plus NB-UVB on vitiligo can enhance effects and shorten course.

目的：探讨多形外膜蛋白（Pmp）与沙眼衣原体泌尿生殖道感染的相关性。方法选取84例沙眼衣原体泌尿生殖道感染患者作为感染组，同期健康体检者40例为对照组，以Western-blot法检测两组对象血清Pmp蛋白抗体。结果感染组有78例检测出Pmp蛋白抗体阳性，阳性率为92．86％，对照组有6例检测出Pmp蛋白抗体阳性，阳性率为15．00％，两组比较差异有统计学意义（P＜0．01）；感染组Pmp蛋白抗体种类从A～I均有分布，其中阳性率 PmpB-Ab最高，PmpD-Ab最低，对照组检出Pm-pA-Ab、PmpB-Ab、PmpF-Ab、PmpG-Ab ，阳性率普遍低。感染组患者治疗3个月后再次检测沙眼衣原体原体（EB）抗体28例仍然为阳性，56例转阴，比较持续感染患者与转阴患者Pmp蛋白抗体检测结果，PmpA～I 9种类型均有分布，各类型阳性率比较差异无统计学意义（P＞0．05）。结论沙眼支原体泌尿生殖道感染患者体内血清均存在Pmp蛋白抗体，不同种类间免疫原性有一定差异，Pmp蛋白抗体的免疫保护能力有限。

Objecttive To explore clinical effective of eclipta capsule and NB-UVB combined with mental interven tion in the treatment of vitiligo.Methods 180 patients with vitiligo were randomly divided into the control group and the treatment group,90 cases of each group.The control group was treated with oral eclipta capsules and NB-UVB irradiation;The treatment group was treated with psychological intervention on the basis of the control group.To evaluate effect through the evaluation of mental health status:dermatology life quality index(DLQI),symptom checklist 90(SCL-90),self-rating depression scale(SDS),self-rating anxiety scale(SAS),clinical white spot number and complex color before and after treatment.Results The total marked effective rate and total effective rate of the treatment group were significantly better than that of the control group(P<0.05).The evaluation of DLQI,SCL-90,SDS,SAS after treatment were better than that of the control group(P<0.05).Conclusion Eclipta capsules and NB-UVB combined with mental intervention in the treatment of vitiligo has quick effect,can shorten the course of treatment and improve the life quality of the patients.

目的探讨儿童尖锐湿疣原因及对策与临床疗效的关系。方法 24例儿童尖锐湿疣患者分别总结分析发病原因及相应对策并采用液氮冷冻联合口服薏仁合剂外用干扰素凝胶及健康宣教综合治疗6～8周,3个月后统计疗效,6个月后统计复发率。结果 24例患儿治愈21例,治愈率为87.5%。6个月后复发2例,复发率为9.5%。结论儿童尖锐湿疣多间接传染而来,不除外性传播,应加强宣教,积极治疗外阴肛周疾病,选择不良反应小,儿童易接受的综合治疗。

Objective:To observe the curative effect of drug combined with acupoint injection therapy for Ramsay Hunt syndrome and prevention sequela.Methods:Patients of Ramsay Hunt syndrome were randomly divided into treatment group of 32 cases and control group of 16 cases.The control group using acyclovir and prednisone acetate tablets treatment,the treatment group was treated with Acupoint Injection on the basis of control group,2 weeks for 1 courses,2 courses were observed,follow-up 3 months.Results:The difference of efficiency rate was statistically significant between the two groups(P<0.05);The difference of VAS score was statistically significant too(P<0.05).Conclusion:Drugs combined with acupointure point injection has a rapid onset,high cure rate,low occurrence rate of sequelae in the treatment of ramsay hunt syndrome.

目的:观察复方地骨皮洗剂治疗角化过度型手足癣的临床疗效。方法:180例患者随机分为治疗组90例,采用复方地骨皮洗剂加外用药,对照组90例单纯外用药;2周为1疗程,治疗2疗程后统计疗效,痊愈者随访1年观察复发率。结果:治疗组治愈率56.67%、显效率77.78%明显高于对照组,复发率15.56%低于对照组,两组比较有显著性差异(P<0.05)。结论:复方地骨皮洗剂治疗角化过度型手足癣疗效确切,无不良反应,复发率低。

Objective To discuss the relationship between acupoint injection of early intervention and herpes zoster and postherpetic neuralgia(PHN).Methods Totally 240 cases of herpes zoster patients were randomly divided into control group(n=80) given by intravenous acyclovir and the semiconductor laser irradiation.The treatment group(160 cases) were given vitamin B1 and cobamamide acupoint injection in addition once a day,10 days in a course.Observe pain score,sleep quality score,pain frequency and comprehensive assessment of curative effect before and after treatment.Follow up of 3 months PHN incidence statistics.Results The cure rate of treatment group,the total efficiency and comprehensive assessment after treatment were significantly better than thaseof the control group,the incidence of PHN were lower than those of control group(P0.05) after 3 months.Conclusions Acupoint injection of early intervention in the treatment of herpes zoster can rapidly relieve the pain,the cure rate is higher and the incidence of PHN is lower.

目的探讨三联疗法治疗早期带状疱疹预防后遗神经痛临床疗效观察。方法 180例带状疱疹患者随机分为治疗组(120例)和对照组(60例),2组均给予阿昔洛韦静脉滴注,半导体激光照射,治疗组联合穴位注射。治疗前及治疗后1、3、7、14 d分别进行视觉模拟评分法(visual analogue scale,VAS)、睡眠质量评分法(sleep quality score,QS)、疼痛频率(frequency,F=次/d)综合评估临床疗效,1个月后观察带状疱疹后遗神经痛(postherpetic neuralgia,PHN)发生率。结果治疗组痊愈率,总有效率及综合评分(VAS,QS,F)均明显优于对照组,PHN发生率低于对照组,差异有统计学意义(P<0.05)。结论三联疗法治疗早期带状疱疹起效迅速,治愈率高,无不良反应,可减少PHN发生率。

目的观察中西医结合治疗角化过度型足癣的临床疗效。方法将120例患者随机分为治疗组和对照组。对照组采用特比萘芬治疗,治疗组加用中药洗剂。结果治疗组痊愈率、总有效率均优于对照组;复发率低于对照组。结论中西医结合治疗角化过度型足癣起效迅速,治愈率高,复发率低。

目的 评价质子泵抑制剂(PPI)隔日疗法及每日疗法治疗非甾体抗炎药(NSAIDs)所致药物性消化性溃疡(PU)的临床疗效.方法 分析86例因服用NSAIDs引起胃、十二指肠溃疡的病例资料给予质子泵抑制剂隔日疗法及每日疗法治疗效果.结果 质子泵抑制剂隔日疗法及每日疗法均有明显效果,两者比较差异无统计学意义.结论 质子泵抑制剂隔日疗法治疗NSAIDs所致PU不仅经济,且减少了由之引起的不良反应,但是增加了患者的依从性.

Aim:To explore the optimal conditions for bio-engineering of human epidermis.Methods:The NIH-3T3 cells were used as a feeder layer. After the epidermis was isolated from dermis with thermolysin. The single epidermal cells were separated by 4 kinds of digesting solutions;0.5 g/L trypsin (T)+0.2 g/L EDTA(E)for group A,2.5 g/L T +0.2 g/L E for group B, 0.5 g/L T+1 g/L E for group C and 2.5 g/L T+1 g/L E for group D. The epidermal cells were plated in DMEM-F12 complete medium containing 8 kinds of contents. The colony formation rate, expanding fold and the confluent time of epidermal cells were recorded.Results: The colony formation rate and expanding fold of the plated epidermal cells in the digesting solution groups respectively were: group A, (10.00±0.09)% and (500.00±9.63)fold; group B, (7.00±0.12)% and (350.00±6.16) fold; group C,(17.00±0.18)% and (850.00±8.83) fold and group D,(14.00±0.19)% and (700.00±9.55) fold. There was significant difference in all of the 4 groups. The confluent time of the seeded epidermal cells was (10.00±0.82) th day in group A, (10.00±0.82) th day in group B,(11.50±1.29) th days in group C and (12.00±2.45)th day in group D. There was no significant difference in correlation between the confluent time and colony formation rate of the seeded epidermal cells in 4 groups.Conclusion: In comparison of the digesting solution groups with the same concentration of EDTA, the bio-effects of groups with 0.5 g/L T were better than those with 2.5 g/L T.