目的 探讨不同头高足低俯卧位通气角度对重型新型冠状病毒感染(COVID-19)患者血流动力学和氧合指标的影响.方法 选择2022年1-12月郑州大学第一附属医院收治的162例重型COVID-19患者,采用随机数字表法将患者分为0～5°组(38例)、10°组(40例)、30°组(43例)及45°组(41例).利用电动病床调节床面倾斜角度实施头高足低俯卧位通气.通气后6 h和12 h,比较各组患者平均动脉压(MAP)、心率(HR)、中心静脉压(CVP)、动脉血氧分压(PaO2)和二氧化碳分压(PaCO2)以及氧合指数(PaO2/FiO2).结果 俯卧位后6 h,各组MAP、HR及CVP比较,差异无统计学意义(P＞0.05);俯卧位后12 h,各组MAP和CVP比较,差异无统计学意义(P＞0.05),30°组和45°组HR低于0～5°组和10°组(P＜0.05).俯卧位后6 h和12 h,各组PaO2、PaCO2及PaO2/FiO2比较,差异有统计学意义(P＜0.05),且 30°组、45°组 PaCO2低于0～5°组和 10°组,PaO2/FiO2高于0～5°组和 10°组(P＜0.05);30°组、45°组 PaO2高于0～5°组(P＜0.05);30°组和45°组各指标比较,差异无统计学意义(P＞0.05).结论 30°、45°头高足低俯卧位通气能较好地改善重型COVID-19患者的氧合状态,效果较0～5°或10°头高足低俯卧位更明显,但对血流动力学的改善不明显.

Objective:To observe the effect of mirror visual feedback training on upper limb function and muscle tension in children with spastic hemiplegia resulting from cerebral palsy (SHCP).Methods:Seventy-six children aged 2-5 with SHCP were randomly divided into a control group of 33 and a treatment group 34. All were given routine occupational therapy, physical therapy, massage and physical agents. Each therapy session lasted 30 minutes daily, 5 times a week over 3 weeks as a course of treatment. There was a one week interval after each of 6 courses, so the total treatment lasted 6 months. The treatment group was additionally trained with mirror visual feedback with the same schedule. Before, as well as after 3 and 6 months of treatment, each patient′s upper limb motor function, fine motor function and muscle tone were evaluated using the Fugl-Meyer motor function assessment scale (FMA), the Peabody fine motor development scales (PDMS-FM), the modified Ashworth scale (MAS) and integrated electromyograms (iEMGs).Results:There were no significant differences between the two groups before treatment. After both 3 and 6 months significant improvement was observed in both groups′ average FMA score, PDMS-FM total score, grip, and visual motor integration. At both points the treatment group′s averages were significantly better than those of the control group. The average MAS and iEMG results, however, were not significantly different at either time point.Conclusions:For children with spastic hemiplegia caused by cerebral palsy, mirror visual feedback training can effectively improve upper limb functioning, but it cannot reduce their muscle tone.

目的 研究胸腔镜下肺癌根治术治疗早期非小细胞肺癌(NSCLC)的效果及对患者肺功能的影响.方法 将86例早期NSCLC患者根据手术方式不同分为观察组(完全胸腔镜下肺癌根治术)66例和对照组(小切口开胸肺癌根治术)20例,比较两组患者围手术期指标、术后并发症发生情况、术后1个月及术后6个月肺功能恢复情况以及随访1年后患者生活质量.结果 观察组患者术中出血量明显少于对照组,引流管放置时间、住院时间均明显短于对照组,术后24 h视觉模拟评分法(VAS)评分明显低于对照组(P﹤0.01).观察组患者并发症总发生率低于对照组(P﹤0.05).术后6个月,观察组患者肺活量(VC)和第1秒用力呼气容积(FEV1)均高于术后1个月及对照组(P﹤0.05).术后,两组患者生活质量各维度评分均高于术前,且观察组生活质量各维度评分均高于对照组(P﹤0.05).结论 胸腔镜下肺癌根治术能有效提高早期NSCLC的治疗效果,促进患者肺功能恢复,减少并发症的发生,改善患者术后生活质量.

Objective:To investigate the effect of exercise-based cardiac rehabilitation after percutaneous coronary intervention (PCI) on patients with myocardial infarction.Methods:One hundred patients with myocardial infarction undergoing PCI in Henan Provincial People’s Hospital from February 2020 to February 2021 were selected. And they were divided into exercise-based cardiac rehabilitation group and routine rehabilitation guidance group according to postoperative intervention methods, with 50 cases in each group. The cardiac function, metabolic equivalent (METs), 6-minute walk distance (6MWD), activities of daily life, major adverse cardiovascular events and bleeding events of the two groups were compared and analyzed.Results:After intervention, wall motion score index, left ventricular end-diastolic diameter and left ventricular end-systolic diameter in the two groups were lower than those before intervention, and these indexes in the exercise-based cardiac rehabilitation group were lower than those in the routine rehabilitation guidance group ( P<0.05); after intervention, the left ventricular ejection fraction, METs and 6MWD in the two groups were higher than those before intervention, and these indexes in the exercise-based cardiac rehabilitation group were higher than those in the routine rehabilitation guidance group ( P<0.05). After intervention, the physical activity, worry about medication, drug adverse reaction, emotional reaction, dependence, safety, diet score and myocardial infarction dimensional assessment scale total score in the two groups were lower than those before treatment (all P<0.05), and these indexes in the exercise-based cardiac rehabilitation group were lower than those in the routine rehabilitation guidance group ( P<0.05). The incidence of major adverse cardiovascular events in the exercise-based cardiac rehabilitation group was 6.00% (4/50), lower than the 22.00%(11/50) in the routine rehabilitation guidance group ( P<0.05). But there was no significant difference in the incidence of bleeding events between the two groups ( P>0.05). Conclusions:Exercise-based cardiac rehabilitation after PCI for myocardial infarction can effectively improve the cardiac function of patients, and effectively improve the quality of life of patients.

目的 了解脑瘫患儿高危因素、临床特点及其与癫痫发生的关系,探讨脑瘫患儿共患癫痫的危险因素.方法 回顾性收集从2019年1月-2022年2月在郑州大学第三附属医院住院治疗的脑瘫患儿的病例资料,将患儿分为脑瘫共患癫痫组和未共患癫痫组,采用x2检验分析两组间高危因素、临床特点等资料,应用多因素Logistic回归对共患癫痫的危险因素进行分析.结果 共纳入630例脑瘫患儿,其中男421例,女209例;脑瘫共患癫痫组患儿155例,未共患癫痫组患儿475例,癫痫发生率24.6％.脑瘫共患癫痫组患儿在宫内窘迫、窒息、多胎及新生儿先天性畸形史方面的发生率高于未共患癫痫组患儿(x2=4.788、9.368、5.255、12.111,P＜0.05),两组患儿在脑瘫分型、头颅MRI分类、粗大运动功能分级方面差异有统计学意义(x2=213.686、14.640、481.531,P＜0.05).多因素Logistic回归结果显示,痉挛型四肢瘫(OR=14.090,95％CI:1.950～101.813)、新生儿先天性畸形史(OR=1.891,95％CI:1.155～3.095)、围生期窒息史(OR=1.527,95％CI:1.034～2.255)可能是脑瘫患儿共患癫痫的危险因素(P＜0.05).结论 脑瘫患儿共患癫痫的发病率较高,病情重,识别危险因素可以为针对性的临床治疗提供依据.

Objective:To retrospectively analyze the clinical effects and complications of alcohol inactivation, irradiation inactivation, and liquid nitrogen inactivation in the treatment of femur osteosarcoma in children, in an attempt to provide a theoretical basis for clinical selection of in vitro inactivation methods of tumor bone segment. Methods:The clinical data of 93 children with femur osteosarcoma admitted to the Department of Bone and Soft Tissue, the Affi-liate Cancer Hospital of Zhengzhou University from January 2008 to December 2017 were retrospectively analyzed, and 40 children, including 21 males and 19 females, aged 8-18 (13.65±2.87) years, who were treated with in vitro inactivation and replantation of autogenous tumor bone segment, were screened.Among these children, there was alcohol inactivation in 15 cases, irradiation inactivation in 12 cases, and liquid nitrogen inactivation in 13 cases.A comparison was drawn on these 3 inactivation methods with respect to bone healing time, bone healing rate, tumor recurrence rate, infection rate, fracture or fixation failure rate, and revision rate. Results:All those 40 children were subject to valid medical followed-up, with the alcohol inactivation group for (102.60±16.55) months, the irradiation inactivation group for (59.33±6.39) months, and the liquid nitrogen inactivation for (36.85±6.49) months.The difference in follow-up time of 3 groups was statistically significant ( P<0.05). Compared with other 2 groups, the index of bone healing time, bone healing rate, infection rate and revision rate in the alcohol inactivation group were unfavorable, which showed a significant difference (all P<0.05); However, there was no significant difference in the recurrence rate, fracture rate or fixation failure rate compared with other 2 groups (all P>0.05); There was no significant difference in all above indexes between the irradiation group and the liquid nitrogen group (all P>0.05). Conclusions:Three in vitro inactivation methods for the treatment of tumor bone segment are safe and reliable.The alcohol inactivated bone has a long healing time and more complications.Both irradiation inactivation and liquid nitrogen inactivation are clinical options, but irradiation inactivation requires professional equipment, which may limit the clinical application.

目的 探讨谷氨酰胺联合早期肠内营养治疗急性重症胰腺炎的疗效.方法 选取2017年9月至2020年9月期间郑州大学第二附属医院收治的65例重症急性胰腺炎患者,随机分为对照组(32例)和谷氨酰胺组(33例).对照组实施早期肠内营养治疗,谷氨酰胺组在对照组治疗基础上联合谷氨酰胺治疗,对比两组疗效、营养状态改善情况等指标.结果 谷氨酰胺组总有效率(93.94％,31/33)显著高于对照组(71.88％,23/32),差异有统计学意义(P<0.05).结论 早期肠内营养治疗基础上,联合谷氨酰胺治疗,可有效提升救治效果,改善患者营养状态及病情危重程度,临床应用效果可靠.

目的 观察补肾健脑针法联合运动疗法对痉挛型脑瘫患者精细运动功能及血清神经生长因子(NGF)、脑源性神经营养因子(BDNF)水平的影响.方法 将106例痉挛型脑瘫患者,采用随机数字表法分为针刺组和运动组,每组53例.运动组采用运动疗法治疗,针刺组采用补肾健脑针法联合运动疗法治疗.观察两组治疗前后精细运动功能及血清神经生长因子(NGF)、脑源性神经营养因子(BDNF)水平变化,并比较脑瘫严重程度.结果 治疗1个月后及治疗后,两组精细运动功能(FMFM)评分、粗大运动功能(GMFM)评分均较治疗前升高(P<0.05),针刺组高于运动组(P<0.05).治疗后,两组血清NGF、BDNF水平均升高(P<0.05),针刺组高于运动组(P<0.05);针刺组脑瘫严重程度分布优于运动组(P<0.05).结论 补肾健脑针法联合运动疗法可减轻痉挛型脑瘫患者病情严重程度,提高精细运动与粗大运动功能,其机制可能与升高血清NGF、BDNF水平有关.

目的 应用医疗失效模式和效应分析(H FM EA)找出肝移植患者中心静脉导管维护操作流程中的失效环节并制订改进措施,规范中心静脉导管相关操作流程标准,以确保患者安全.方法 选取2019年1月至2020年3月在该院外科重症监护室行肝移植且符合纳入标准的180例患者作为研究对象,根据H FM EA模式实施情况分为两组,2019年1～8月为对照组(n=90),2019年9月至2020年3月为试验组(n=90).比较两组患者实施HFMEA管理前后危机值、导管相关性血流感染(CRBSI)发生率和护士对实施HFMEA管理的评价情况.结果 危机值由H FM EA管理实施前的2206分降至实施后的732分,危机值下降率为66.8％;试验组患者CRBSI发生率(2.3％)明显低于对照组(10.1％),差异有统计学意义(P<0.05);两组护士对实施HFMEA管理后导管维护管理的评价差异有统计学意义(P<0.05).结论 HFMEA管理理论在中心静脉导管维护流程中起优化作用,能有效降低CRBSI发生率,实现持续改进导管相关护理质量的目的.

目的 检测微小RNA-138(miR-138)、微小RNA-338(miR-338)在痉挛型脑性瘫痪患儿中表达水平,并探究两者的临床价值.方法 选取2018年3月-2020年4月于本院门诊就诊及住院治疗的64例痉挛型脑性瘫痪患儿为研究对象(痉挛型脑性瘫痪组);同期选取62例健康体检儿童作为健康对照组.采用实时荧光定量PCR (qRT-PCR)法检测各组受试儿童血清中miR-138、miR-338表达水平;采用酶联免疫吸附(ELISA)法检测各组受试儿童血清肿瘤坏死因子-α(TNF-α)、C-反应蛋白(CRP)、神经生长因子(NGF)、内皮素(ET)水平;采用Pearson法分析痉挛型脑性瘫痪患儿血清miR-138、miR-338与TNF-α、CRP、NGF、ET表达水平相关性;采用受试者工作特征曲线(ROC)分析血清miR-138、miR-338水平对痉挛型脑性瘫痪的诊断价值;采用多因素Logistic回归分析影响痉挛型脑性瘫痪发生的因素.结果 痉挛型脑性瘫痪组血清中miR-138、miR-338、TNF-α、CRP、ET表达水平高于健康对照组,NGF表达水平低于健康对照组,差异均有统计学意义(t=9.598、9.592、11.039、42.397、14.172、5.744,P＜0.05).痉挛型脑性瘫痪患儿血清miR-138与miR-338呈正相关(r=0.687,P＜0.05),且二者均与TNF-α、CRP、ET水平呈正相关(miR-138:r=0.519、0.483、0.494;miR-138:r=0.506、0.492、0.516,P＜0.05),与NGF水平呈负相关(r=-0.522、-0.496,P＜0.05).血清miR-138、miR-338诊断痉挛型脑性瘫痪的曲线下面积(AUC)分别为0.882、0.858,截断值分别为1.189、1.406,特异性分别为75.8％、93.5％,敏感度分别为87.5％、67.2％;二者联合诊断痉挛型脑性瘫痪的AUC为0.950,特异性为90.3％,敏感度为93.8％.Logistic回归显示miR-138、miR-338是痉挛型脑性瘫痪发生的危险因素[OR(95％CI)分别为2.280(1.742～2.983)、2.222(1.637～3.016)],NGF是痉挛型脑性瘫痪发生的保护因子[OR(95％ CI)为0.720(0.527～0.984),P＜0.05].结论 痉挛型脑性瘫痪患儿血清中miR-138、miR-338表达水平显著上调,二者对痉挛型脑性瘫痪的发生、发展可能有重要提示作用.

目的:观察重复经颅磁刺激联合强制性诱导疗法治疗偏瘫型脑瘫患儿的效果.方法:选取100例偏瘫型脑瘫患儿为研究对象,按随机数字表法分为研究组和对照组各50例.两组均给予常规康复治疗,在此基础上,对照组采用强制性诱导疗法,研究组在对照组基础上采用重复经颅磁刺激治疗.比较两组治疗前后Peabody精细运动发育量表-2(PDMS-2FM)和Carroll双上肢功能测试(UEFT)量表评分.结果:治疗后,研究组PDMS-2FM和UEFT评分均高于对照组,差异有统计学意义(P<0.05).结论:重复经颅磁刺激联合强制性诱导疗法治疗偏瘫型脑瘫患儿可提高PDMS-2FM和UEFT评分,效果优于单用强制性诱导疗法.

目的 筛选食管癌放疗后中医辨证要点,优化食管癌放疗后的中医辨证治疗.方法 编制食管癌中医证候研究专家问卷,用德尔菲法对全国18个省的一线临床肿瘤专家进行咨询,共识会议法修订,SPSS 21.0软件分析专家积极程度、专家意见集中程度、专家意见协调程度,筛选食管癌放疗后中医证候辨证要点.结果 食管癌放疗后中医证候热毒蕴结、胃气失和证保留14个辨证要点;肺胃阴伤、气血虚弱证保留14个辨证要点.结论 明确了食管癌放疗后中医证候辨证要点,为临床食管癌放疗后辨证治疗方案的优化提供参考依据.

〔目的〕探讨踝关节持续被动运动在脑瘫足外翻患儿中的应用效果.〔方法〕收集100例脑瘫足外翻患儿为研究对象,随机分组后分为研究组与对照组,对照组患儿给予综合康复治疗,研究组患儿在综合康复治疗的基础上给予踝关节持续被动运动(CPM)治疗,治疗4个疗程后观察与比较两组患儿的站立及走、跑、跳能力及临床治疗效果.〔结果〕干预后两组患儿的站立及走、跑、跳能力比干预前均有明显的改善,且研究组患儿改善的更加明显.干预后研究组患儿的临床疗效明显高于对照组.〔结论〕踝关节持续被动运动能明显改善脑瘫足外翻患儿的站立及走、跑、跳能力,明显提高患儿的临床疗效.

Objective:To investigate the relationship of expression of serum neuron specific enolase (NSE) and S100β protein with short-term brain function prognosis of patients with successful cardiopulmonary resuscitation.Methods:The clinical data of 109 patients who successfully underwent cardiopulmonary resuscitation in Henan Provincial People’s Hospital from January 2015 to October 2020 were retrospectively analyzed. According to the brain function assessed by cerebral performance category (CPC), the patients with CPC grade 1-2 (85 cases)were included in the good prognosis group, and the patients with CPC grade 3-5 (24 cases) were included in the poor prognosis group. The general data, levels of NSE and S100β protein were compared between the two groups. Logistic regression analysis was used to analyze the correlation of expression of NSE and S100β protein with short-term prognosis of patients with successful cardiopulmonary resuscitation.Results:There was no statistically significant different in general data between the two group ( P>0.05). Serum NSE and S100β protein in poor prognosis group were(112.58±24.46)μg/L and(1.84±0.36)ng/L, respectively, higher than (25.46±6.52)μg/L and (1.02±0.25)ng/L in good prognosis group ( P<0.05). Logistic regression analysis showed that the expression of NSE and S100β protein was related to the short-term prognosis of cerebral function in patients with cardiopulmonary resuscitation ( P<0.05). Conclusions:Overexpression of NSE and S100β protein may lead to poor prognosis of brain function in patients with successful cardiopulmonary resuscitation.

目的:探讨使用螺旋断层进行全身皮肤照射治疗难治性皮肤T细胞淋巴瘤中蕈样霉菌病的方法.方法:具有7年病史的男性患者确诊为全身多发皮肤T细胞淋巴瘤中的蕈样霉菌病,行全身皮肤照射,采用5 mm氯丁橡胶潜水衣作为组织补偿物,利用其剂量建成效应提高全身皮肤剂量.患者分上下两段扫描,髌骨上方10 cm附近作为分界线,总处方剂量24 Gy,单次1.2 Gy执行20次,5次/周.计划设计采用射野宽度5 cm,螺距0.287,调制因子2.5,在体中心加入全挡模式以减少内部危及器官受量,尤其是全身骨髓剂量,以降低骨髓抑制的发生率.通过Cheese Phantom点剂量、ArcCHECK三维面剂量及全身多点EBT3胶片验证,确保剂量投照的准确性.最后利用兆伏级图像多部位引导确保摆位和治疗的精度.结果:上段靶区和下段靶区均实现95％靶区体积达到处方剂量,下段靶区的适形度指数和均匀性指数略优于上段靶区.全身骨髓随着距离皮肤间距的缩小而受量逐步增加,其余危及器官剂量均在临床可接受范围之内.点剂量验证偏差小于1％,三维面剂量验证Gamma通过率大于90％,胶片多点剂量验证偏差小于5％,多种验证确保实际投照剂量的准确性,整个治疗过程时间约1h(包括摆位和图像引导),患者治疗后期仅出现轻微的乏力、恶心、呕吐、低热及Ⅲ度骨髓抑制,整个治疗效果接近完全缓解.结论:该方法具有剂量分布均匀、治疗时间短、实施过程简易、治疗效果佳和毒副作用低的特点,适合在临床中广泛开展,但对剂量建成层的组织补偿物选择及治疗后的毒副作用尤其是骨髓抑制需进一步探讨.

电视节目《典籍里的中国》以创新手法将中华典籍搬上荧屏,为文化经典的当代化传播做出了有益尝试.节目综合了专家访谈、剧作围读、戏剧演绎等多种表现手法,以人物为中心,以情感引共鸣,建构起文化传承的仪式,并在今人与古人的跨时空对话中完成对先贤的求问与告慰,其深厚的文化底蕴、新颖的表现形式、精良的制作品质可圈可点,让传统典籍成功走进了大众的视野.

大学生是重要的人才资源,其健康成长意义重大,通过廉洁教育,能够促进大学生形成廉洁自律、遵纪守法、无私奉献等优秀品质.以故事化的形式进行廉洁教育,契合大学生的思想和心理特点,能够提高教育的吸引力、感染力和说服力.高校教育工作者应积极探索和实践,利用多种渠道和平台,在以故事化的形式对大学生进行廉洁教育的过程中做到五个相结合.

目的:探讨缝匠肌肌瓣移位联合VSD在腹股沟淋巴结清扫术的应用,及共对降低术后皮缘坏死、淋巴漏及切口感染等并发症的影响。方法:回顾性分析2016年7月至2018年6月行腹股沟淋巴结清扫术的足部恶性黑色素瘤患者50例；其中采用单纯腹股沟淋巴结清扫术的25例为对照组；采用腹股沟淋巴结清扫、缝匠肌肌瓣移位加VSD敷料覆盖创面的25例为试验组。术后定期随访,观察两组患者在术后发生切口感染、淋巴漏及皮缘坏死等并发症发生率,并进行统计学分析, P<0.05为差异有统计学意义。 结果:本组50例术后肌瓣均顺利成活,切口一期愈合。50例均获得随访,试验组术后随访时间14～90（40.12±30.68） d，对照组术后随访时间为14～120（42.20±34.15） d。试验组皮缘坏死2例（8.0%),较对照组[10例（40.0%)]明显减少；淋巴漏发生2例（8.0%),较对照组[7例（28.0%)]明显降低;切口感染3例（12.0%),也较对照组[9例（36.0%)]低,上述差异均有统计学意义（ P<0.05) 。 结论:腹股沟淋巴结清扫术中应用缝匠肌肌瓣移位加VSD能明显降低患者术后皮缘坏死、淋巴漏及切口感染的发生率,提高手术效果。

目的 研究三孔胸腔镜肺癌根治术术后慢性疼痛的发生率并探讨其影响因素.方法 收集某三级甲等医院2018年1月1日-2018年10月31日符合研究要求的胸腔镜下肺癌根治术患者56例.实际完成随访48例.以数字分级评分法评定术后第1-3天,第3个月时患者的疼痛程度.以术后第3个月是否发生术后慢性疼痛(CPSP)为标准,将患者分为两组,即CPSP组,与非CPSP组.并针对10项相关因素进行分析.结果 根据实际完成的随访例数分析年龄,术后1-3天疼痛程度差异有统计学意义(P＜0.05).结论 针对存在统计学差异的相关因素行Logistics回归分析,分析结果显示术后急性期疼痛(P＜0.05)、年龄(P＜0.05)是发生慢性术后疼痛的独立危险因素.

Objective:To observe the effects of probiotics and bioelement on intestinal flora in mice with severe acute pancreatitis.Methods:Eighty 6-8-week-old male C57BL/6J mice were randomly divided into four groups, each containing 20 mice. Group A was the blank control group, group B was the modeling control group, group C was the probiotics group, and group D was the symbiotic tuple group. The body weight, pathological score, serum serotonin oxidase (DAO), endotoxin, tumor necrosis factor-α (TNF-α), interleukin (IL)-6 concentrations of the mice in each group were compared, and the relative abundance of intestinal mucosa and fecal samples in each group was compared.Results:At first week, weight of mice in groups B, C and D was lower than that in group A [(35.11±0.70) g], and that in group C [(33.46±0.72) g] and group D [(33.50±0.89) g] than in group B [(30.35±0.89) g]. The pathological scores in group B (27.00±7.94), group C (5.40±2.79) and group D (7.25±2.85) were higher than in group A (0), and those in groups C and D were lower than in group B, with the difference being statistically significant ( F=7.586, 5.365, P< 0.05). Serum DAO [(1.13±0.21), (1.00±0.32), (0.98±0.21) U/L], endotoxin [(3.48±0.07), (2.56±0.10), (2.55±0.05) EU/L], TNF-α [(21.07±9.09), (16.24±2.52), (15.90±8.43) ng/L], and IL-6 [(24.96±8.89), (20.41±6.02) ng/L, (19.02±7.40) ng/L] were significantly higher groups B, D and D than those in group A, and those in groups C and D were significantly higher than those in group B ( F=8.427, 6.536, 4.151, 6.158, P< 0.05). Alpha diversity analysis showed that the fecal and intestinal mucosal flora biodiversity of mice in groups B, C and D was significantly less than that in group A ( F=2.084, 2.823, 2.557, 2.186, 2.573, 1.983, P< 0.05). Principal Component Analysis (Principal Component Analysis, PCA) showed that the fecal flora of mice in each group was compared, and the differences were statistically ( t=2.279, 2.559, 2.495, 1.975, 2.557, 2.084, P< 0.05). Conclusion:Probiotics and synbiotics can reduce the degree of intestinal inflammation in mice with severe acute pancreatitis, regulate the proportion of beneficial bacteria, maintain the balance of intestinal flora, and protect the intestinal barrier and absorption function.