为了建立一种能快速鉴别猪日本脑炎病毒(JEV)、猪繁殖与呼吸综合征病毒(PRRSV)、猪细小病毒(PPV)和猪伪狂犬病病毒(PRV)的准确、高效的多重常规PCR检测方法,针对JEV、PRRSV、PPV、PRV基因保守区域合成特异性引物,优化后获得最优反应条件,对该方法特异性、敏感性、重复性进行了测定,并应用该方法对临床样品进行初步应用.结果显示,该方法对JEV、PRRSV、PPV、PRV可进行特异性扩增,对混合阳性质粒检测下限达2×106 copies/μL,对猪圆环病毒2型、猪瘟病毒、猪流行性腹泻病毒、猪丁型冠状病毒、猪传染性胃肠炎病毒等相关病毒均无扩增,且重复性良好.用该方法检测51份2021年采集的广西区内组织样品,检出JEV、PRRSV、PPV、PRV阳性率分别为7.84％、50.98％、5.88％、23.53％,且存在多重混合感染的情况.所建立的多重PCR方法具有良好的特异性、敏感性及重复性,可应用于临床常见猪繁殖障碍性病毒病的快速诊断和监测预警,为猪繁殖障碍性病毒病提供了诊断技术支持.

旨在研究猪丁型冠状病毒(porcine deltacoronavirus,PDCoV)N蛋白的抗原性,为下一步诊断试剂盒的研发提供依据.试验通过RT-PCR的方法扩增PDCoV CH/GX/1468B/2017株完整的N基因并构建原核表达质粒pET32a-N,将pET32a-N转化至大肠杆菌BL21感受态细胞中,经诱导表达获得重组N蛋白,纯化后经Western blot方法检测其反应原性,免疫昆明小鼠制备重组N蛋白多克隆抗体并进行效价测定(间接ELISA法)和特异性验证(间接免疫荧光法).结果显示:重组N蛋白在IPTG终浓度为1.0mmol/L,37℃诱导表达6 h的条件下可获得最高表达量,该重组蛋白主要以可溶性蛋白的形式表达;Western blot结果显示,纯化后的重组N蛋白可以和PDCoV阳性猪血清发生特异性结合,具有良好的反应原性.制备的多克隆抗体的效价可达1:64 000,间接免疫荧光法结果表明其能特异性识别和结合PDCoV,说明重组N蛋白具有较好免疫原性.提示:PDCoV N蛋白抗原性好,可作为诊断试剂盒的候选抗原.

Wumei San (WMS) is a traditional Chinese medicine that has been widely applied in the treatment of piglet diarrhea (PD). However, the mechanism of WMS in PD has not been investigated. In this study, the main active compounds of WMS and the target proteins were obtained from the Traditional Chinese Medicine Systematic Pharmacology, PubChem, and SwissTargetPrediction databases. The molecular targets of PD were identified using GeneCards, OMIM, and NCBI databases. The common targets of WMS and PD were screened out and converted into UniProt gene symbols. PD-related target genes were constructed into a protein-protein interaction network, which was further analyzed by the STRING online database. Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes enrichment analyses were performed to construct the component-target gene-disease network. Molecular docking was then used to examine the relationship between the core compounds and proteins. As a result, a total of 32 active compounds and 638 target genes of WMS were identified, and a WMS-compound-target network was successfully constructed. Through network pharmacology analysis, 14 core compounds in WMS that showed an effect on PD were identified. The targets revealed by GO and KEGG enrichment analysis were associated with the AGE-RAGE signaling pathway, PI3K-Akt signaling pathway, TNF signaling pathway, NOD-like receptor signaling pathway, IL-17 signaling pathway, and other pathways and physiological processes. Molecular docking analysis revealed that the active compounds in WMS spontaneously bind to their targets. The results indicated that WMS may regulate the local immune response and inflammatory factors mainly through the TNF signaling pathway, IL-17 signaling pathway, and other pathways. WMS is a promising treatment strategy for PD. This study provides new insights into the potential mechanism of WMS in PD.

目的:益生菌发酵鸽粪生产饲料研究.方法:本文用复合益生菌(枯草芽孢杆菌、酵母菌、乳酸菌、放线菌、放线菌等)、鸽粪、玉米粉、麸皮和豆粕混合发酵10 d,比较发酵鸽粪前后的品质变化.结果:经过复合益生菌发酵后不添加辅料组和添加辅料组粪臭味变成酸香味,质地变松软,pH值下降,粗蛋白和粗纤维含量少量提升.结论:通过复合益生菌发酵鸽粪生产饲料,可改善鸽粪适口性,消除鸽粪异味,维持鸽粪营养价值,为生产廉价鸽粪蛋白质饲料提供依据.

制备桃金娘果多糖并对其单糖组成进行分析,探讨桃金娘果多糖对健康小鼠免疫功能的影响.采用水浴提取法制备桃金娘果多糖,衍生化高效液相色谱法分析单糖成分.选用健康昆明小鼠80只,随机分为空白组、黄芪多糖组(腹腔注射200 μg/g·BW)、桃金娘果多糖低、中、高(分别腹腔注射50、100、200 μg/g·BW)共5组.空白组每只小鼠腹腔注射等体积的生理盐水,1次/d,连续7d.试验结束后,分别测定小鼠脏器指数(胸腺、脾脏)、血清中IL-1β、IL-2、IL-6、IFN-γ、LZM、POD等免疫指标的含量.结果显示,桃金娘果多糖含量为65.83％,主要由甘露糖、鼠李糖、半乳糖醛酸、葡萄糖、半乳糖、木糖、阿拉伯糖组成.桃金娘果多糖会降低小鼠的胸腺指数,但能显著增强小鼠的脾脏指数;桃金娘果多糖可提高了IL-1β、IL-2、IL-6、IFN-γ血清细胞因子的水平、POD活性和LZM含量.桃金娘果多糖对提高正常小鼠的脾脏指数及免疫调节功能具有较好效果,但浓度过高会引起小鼠胸腺萎缩,此研究结果为桃金娘的进一步开发和利用提供参考依据.

为建立猪流行性腹泻病毒(PEDV)血清学ELISA抗体检测方法,本研究以原核表达纯化的PEDV S2重组蛋白为包被抗原,通过反应条件优化,建立了一种PEDV重组S2蛋白的间接ELISA抗体检测方法.结果显示,重组蛋白抗原最佳包被量0.8 μg/孔;血清最佳稀释倍数为1 ∶40,最佳血清孵育时间为120 min;酶标二抗最佳工作浓度为1 ∶4000,最佳二抗反应时间为45 min;最佳底物显色时间为15 min;待检血清样品S/P值＞0.212时判定为阳性,待检血清样品S/P值＜0.188时判定为阴性,当0.188≤待检血清样品S/P值≤0.212时判定为可疑.以S2蛋白作为包被抗原建立的PEDV抗体间接ELISA方法仅对PEDV血清检测为阳性,与猪瘟病毒、猪丁型冠状病毒等主要猪源病毒阳性血清均无交叉反应,具有良好的特异性.该方法检测灵敏度较高、重复性好,批内和批间重复性变异系数均小于10％.利用本研究建立的PEDV S2蛋白间接ELISA抗体检测方法对广西地区不同阶段猪群血清样品共计622份进行检测,总体样品阳性率为76.05％,不同阶段阳性率相差较大.本实验建立的ELISA方法可应用于临床样品PEDV抗体检测以及PEDV血清流行病学调查.

试验旨在研究一种合成酯化鸽乳对人工哺育幼鸽生长性能和免疫功能的影响.选取同批孵化出雏的银王鸽幼鸽180只,随机分为3组:1日龄人工哺育组、3日龄人工哺育组、亲鸽哺育组,每组5个重复,每重复12只;试验时间10 d.结果表明:1日龄人工哺育组1～10日龄幼鸽日增重低于亲鸽哺育组(P<0.05),3日龄人工哺育组3～7日龄幼鸽日增重接近于亲鸽哺育组(P>0.05)、8～10日龄幼鸽日增重显著低于亲鸽哺育组(P<0.05);1日龄和3日龄人工哺育幼鸽翅羽长度、翅羽宽度发育程度显著低于亲鸽哺育幼鸽(P<0.05);各组间法氏囊指数差异显著(P<0.05),胸腺和脾脏指数差异不显著(P>0.05);各组间血清免疫球蛋白G(Immunoglobulin G,IgG)含量差异不显著(P>0.05),血清免疫球蛋白M(Immunoglobulin M,IgM)含量差异显著(P<0.05);1日龄人工哺育、3日龄人工哺育血清免疫球蛋白A(Immunoglobulin A,IgA)含量差异显著(P<0.05).综上所述,酯化鸽乳适宜于幼鸽3日龄超前断乳人工哺育,较佳哺育时间为3～7日龄;酯化鸽乳需要在促进幼鸽法氏囊、羽毛生长发育方面进行适当营养调整或补充.

[目的]探讨桃金娘果多糖对免疫抑制小鼠免疫功能的影响.[方法]腹腔注射环磷酰胺80 μg/g BW,1次/d,连续3d,建立小鼠免疫抑制模型.将造模成功的小鼠随机分为5组(每组16只):模型组、黄芪多糖组、桃金娘果多糖低、中、高剂量组.另设16只健康小鼠为正常组.黄芪多糖组每只小鼠按200 μg/g BW剂量腹腔注射黄芪多糖混悬液,桃金娘果多糖低、中、高剂量组分别按50、100、200 μg/g BW剂量腹腔注射桃金娘果多糖混悬液,正常组和模型组每只小鼠腹腔注射等体积的生理盐水,1次/d,连续7d.试验结束后,分别测定小鼠脏器指数(朐腺、脾脏)、血清中白细胞介素1β(IL-1β)、白细胞介素2 (IL-2)、白细胞介素6(IL-6)、γ干扰素(IFN-γ)、溶菌酶(LZM)的含量和过氧化物酶(POD)活性.[结果]桃金娘果多糖不同剂量组小鼠胸腺指数和脾脏指数均有不同程度提高,尤以桃金娘果中剂量组最高,与模型组相比差异极显著(P＜0.01).桃金娘果多糖不同剂量组IL-6、IL-1β血清细胞因子含量均极显著高于模型组(P＜0.01);桃金娘果多糖低、中剂量组的血清IFN-γ、IL-2含量相较于模型组都有所提升,但差异均不显著(P＞0.05),桃金娘果多糖高剂量组的IFN-γ、IL-2含量均极显著高于模型组(P＜0.01).桃金娘果多糖不同剂量组小鼠的POD活性和LZM含量均极显著或显著高于模型组(P＜0.01;P＜0.05).[结论]桃金娘果多糖中剂量即100 μg/g BW对免疫抑制小鼠免疫功能的恢复效果最佳.

砷(As)是一种备受关注的环境化学元素,由于砷矿的开采及砷化合物在农牧业的使用,环境中的砷暴露对人畜的心血管系统产生严重的影响,包括缺血、心律失常和心力衰竭.三氧化二砷(As2 O3)是常见的无机砷化合物,近年来被作为治疗急性早幼粒细胞白血病的高效药,其在临床应用上一直受到心脏毒副作用的限制.目前研究认为,三氧化二砷诱导的心脏毒性可能的机制包括离子通道功能改变、氧化应激、细胞自噬和凋亡.文章就近年来国内外对As2 O3心脏毒性的机制研究及其防治进行综述.

为建立快速、准确的猪丁型冠状病毒(PDCoV)抗体ELISA检测方法,本研究以重组蛋白N作为包被抗原,建立了 PDCoV间接ELISA抗体检测方法.结果显示,经条件筛选,确定了该ELISA最佳反应条件:重组蛋白每孔包被0.2 μg;待检血清1:80稀释,孵育60 min;酶标二抗1:4 000稀释,反应30 min;底物显色10min.待检血清样品S/P值＞0.200时判定为阳性,S/P值＜0.173时判定为阴性,S/P值介于0.173和0.200之间则判为可疑.特异性试验结果表明,该检测方法不与HCV、FMDV、PRRSV、PRV、TGEV和PEDV的阳性血清发生交叉反应;重复性试验结果显示,批内变异系数为0.79％～4.75％,批间变异系数在1.92％～6.60％之间,表明重复性良好;敏感性试验结果显示,该方法可检测到血清最大稀释度为1:320.符合率试验结果显示,本方法与中和试验检测结果符合度高,阳性符合率、阴性符合率和总符合率分别为86.36％、96.43％和92.00％.利用本研究建立的ELISA方法对456份采自广西地区不同阶段猪群的血清样品进行检测,结果显示,总体样品阳性率为40.40％,不同阶段猪群抗体阳性率有较大区别,后备母猪的阳性率可高达100％,保育猪阳性率最低仅为3.90％.

Coinfection caused by various genotypes of porcine epidemic diarrhea virus (PEDV) is a new disease situation. We previously reported the coexistence of PEDV strains containing different ORF3 genotypes in China. In this study, the PEDV strains 17GXCZ-1ORF3d and 17GXCZ-1ORF3c were isolated and plaque-purified from the same piglet, which had a natural large deletion at the 172–554 bp position of the ORF3 gene or possessed a complete ORF3 gene, respectively. Meanwhile, 17GXCZ-1ORF3d had >99% nt identity with 17GXCZ-1ORF3c in the 5′UTR, ORF1a/1b, S, E, M, N and 3′UTR regions but only demonstrated low nucleotide identities (80.5%) in the ORF3 gene. To elucidate the pathogenicity, 7-day-old piglets were infected. Piglets infected with these two PEDV strains exhibited severe clinical signs and shed the virus at the highest level within 96 hpi. Compared with the piglets inoculated with the 17GXCZ-1ORF3c strain, the piglets inoculated with the 17GXCZ-1ORF3d strain had higher mortality rates (75% vs. 50%), an earlier onset of clinical signs with a significantly higher diarrhea score, lower VH:CD ratios and a higher percentage of PEDV-positive enterocytes. This study is the first to report PEDV coinfections with different ORF3 genotypes, and a PEDV strain with a large deletion in the ORF3 gene might have the advantage of a potential genetic marker, which would be useful during vaccine development.

为了解广西猪场伪狂犬病毒(PRV)感染和免疫状况,2017年1月—2020年12月,对部分猪场随机采样送检的血清样品,应用ELISA方法开展PRV血清抗体检测,并对检测结果进行不同年份、不同季节、不同地区、不同生长阶段猪群的统计分析.结果显示:从时间分布上看,2018年PRV gE抗体场阳性率(57.71％)和个体阳性率(24.75％)均最高,此后呈逐年下降趋势,其中个体阳性率下降明显(P<0.05),2020年下降至6.14％;各年间的PRV gB抗体场合格率差异不显著(P>0.05),均在90％以上,而2019年的个体阳性率(88.71％)最低,与其他年份差异显著(P<0.05).冬季PRV gE和gB抗体个体阳性率最低,分别为14.96％和89.35％,与其他季节差异明显(P<0.05);冬季gE抗体场阳性率(42.97％)最低,与春季、秋季差异不显著(P>0.05),但显著低于夏季(P<0.05),而gB抗体场合格率一年四季差异不显著(P>0.05),均在95％以上.从空间分布上看,广西14个地市猪场均存在不同程度的PRV野毒感染,其中玉林市最严重,场阳性率和个体阳性率分别为69.51％和35.86％,而钦州市最轻,分别为16.67％和4.07％;PRV gB抗体个体合格率普遍较高,均在84％以上.从不同生长阶段猪群上看,后备母猪、育肥猪PRV gE和gB抗体个体阳性率均显著低于其他生长阶段猪群(P<0.05),其中gE抗体个体阳性率在13％以下,而gB抗体个体阳性率不足80％.结果表明,近年广西规模猪场PRV野毒感染率较高,而疫苗免疫并不能完全阻止野毒株感染.建议通过PRV净化和提高规模猪场生物安全水平等措施来控制其流行.本研究为制定合理的猪伪狂犬病防控与净化策略提供了依据.

旨在对广西玉林市某规模化猪场免疫猪流行性腹泻病毒(porcine epidemic diarrhea virus,PEDV) AJ1102疫苗株和猪传染性胃肠炎病毒(TGEV)二联弱毒活疫苗后,猪群发生的腹泻进行确诊.通过对发病猪群临床观察、腹泻死亡仔猪病理解剖、实验室RT-PCR检测和基因测序,最终诊断为PEDV变异株感染.对PEDV变异毒株的S基因进行了扩增测序与分析发现,该毒株与国内外参考毒株S基因核苷酸同源性为92.8％～ 99.5％,氨基酸同源性为91.8％ ～ 99.2％;遗传进化分析表明,该PEDV毒株属于G2-a型变异毒株;氨基酸序列比较结果显示,该PEDV变异株氨基酸在多个位点发生了变异,S蛋白在第807位与第827位疏水性氨基酸处出现了较大的变化,在抗原位点第800～813位氨基酸处发生了较为明显的变化.本研究通过该例猪流行性腹泻病毒变异株感染的临床诊断和S基因变异分析,为PEDV病原学研究以及科学防控该病提供参考依据.

为了解广西玉林市牛羊口蹄疫(FMD)和小反刍兽疫(PPR)免疫情况,2017—2019年采用酶联免疫吸附试验(ELISA),对从玉林市7个县(市、区)234个种畜场、规模养殖场和散养户采集2244份羊血清和922份牛血清样本进行O型、A型和亚洲I型FMD以及PPR免疫抗体检测,并比较不同年份、不同地区的免疫抗体水平.结果显示:2017—2019年,玉林市牛羊O型FMD抗体场群及个体合格率基本保持在70％以上,但部分地区的抗体合格率较低(P<0.05);亚洲I型FMD抗体合格率2017年偏低,但2018年后直线上升至80％以上;A型FMD抗体合格率普遍较低,均不足70％.PPR免疫合格率逐年增长,均保持在70％以上,且无显著地区差异(P>0.05).结果表明,广西玉林市牛羊O型、亚洲I型FMD和羊PPR的整体免疫效果较好,但A型FMD免疫效果较差,且FMD免疫效果存在一定的地区不均衡性.因此,玉林市应根据各地区实际情况,持续加强FMD、PPR免疫和监测,确保各地区牛羊处于较高的免疫保护水平.本调查为玉林市有效防控此类疫病提供了技术支持.

近年来,猪德尔塔冠状病毒(porcine deltacoronavirus,PDCoV)给养猪业造成了较大经济损失.为快速检测PDCoV,针对病毒N基因设计特异引物,建立了PDCoV重组酶介导核酸等温扩增荧光法(RT-RAA).通过优化引物浓度、反应温度,确定了最佳反应条件,然后进行了特异性和灵敏性试验,并与普通RT-PCR方法进行了比较.结果显示:该方法特异性较好,与猪繁殖与呼吸综合征病毒、猪轮状病毒、猪伪狂犬病病毒、猪细小病毒、猪圆环病毒、猪瘟病毒、猪捷申病毒、传染性胃肠炎病毒、猪流行性腹泻病毒等猪源病毒无明显交叉反应;反应过程均在39℃恒温条件下完成,用时30 min即可得到扩增结果;反应体系可检测的最低扩增拷贝数为10 copies/μL,敏感性较高;该方法检测68份猪源病毒样品的PDCoV阳性检出率为17.6％,高于普通RT-PCR(13.2％).结果表明,本研究建立的RT-RAA快速检测PDCoV方法具有快速、特异、灵敏的优点,可用于PDCoV的快速检测和流行病学监测.

Radix Rhodomyrti is the dried root of Rhodomyrtus tomentosa (Aiton) Hassk., which is used as a herb in southeast Asian countries and regions. Its main active ingredients are phenols. To make efficient use of the active components in Radix Rhodomyrti, 2,2-diphenyl-1-picrylhydrazyl radical–ultra-high-performance liquid chromatography–photo diode array (DPPH–UPLC–PAD) analysis and UPLC–quadrupole time-of-flight tandem mass spectrometry (UPLC–QTOF-MS/MS) were applied to evaluate and identify the antioxidant phenolic components in Radix Rhodomyrti. Gallic acid and ellagic acid were identified as the major phenolic components in Radix Rhodomyrti. The levels of gallic acid and ellagic acid in Radix Rhodomyrti were 2.16 ± 0.27 mg/g and 0.17 ± 0.04 mg/g, respectively. Both compounds showed excellent free-radical scavenging activity in vitro. The known antioxidant capacity of ellagic acid is slightly higher than that of gallic acid, which is consistent with the results of the DPPH radical-scavenging assay. The results of this study indicate that the proposed method could be applied for rapid screening and identification of antioxidants from complex natural products.

为了解近年来广西玉林市规模猪场主要病毒性疫病的流行动态和免疫保护水平,分析疫情流行趋势和暴发风险,对2016—2019年采集自玉林市规模猪场284个场次的6954份血清样本,以及113个场次的1539份临床健康猪组织样本、249个场次自主送检的536份病死猪组织样本,进行猪瘟病毒(CSFV)、猪繁殖与呼吸综合征病毒(PRRSV)、猪圆环病毒2型(PCV2)、猪伪狂犬病病毒(PRV)抗体和抗原检测,并对结果进行时间和区间分析.结果显示:2016—2019年,规模化猪场CSFV、PCV2、PRV-gB场群平均抗体阳性率以及个体平均抗体阳性率均在70％以上;PRRSV抗体阳性率总体偏低,但2019年抗体阳性率超过90％.北流市CSFV场群及个体抗体阳性率最低,与其他地区有差异(P<0.05);北流市、福绵区、玉州区的PRRSV场群抗体阳性率未超过70％,低于其他地区(P<0.05);各地区间PCV2抗体阳性率无统计学差异(P>0.05);福绵区PRV-gB抗体阳性率(<70％)与其他地区相比最低(P<0.05);在健康屠宰猪群及病死猪群组织样品中均检出上述4种病原,其中PRRSV、PCV2病原阳性检出率较高.结果表明,玉林市规模化猪场上述4种疫病的免疫抗体保护水平总体较高,但部分地区抗体水平偏低,且均存在猪群带毒现象,尤其是PRRSV、PCV2,存在疫病暴发风险.结果提示,各县(市、区)要根据本地养殖及疫病监测情况,制定科学合理的免疫计划,加强综合防控与监测,防止上述猪群疫病的发生与流行.本研究调查了广西玉林市猪群主要病毒性疫病的免疫保护水平及其病原流行特点,为该地区此类疫病防控提供了数据支持.

利用超声辅助提取法对桃金娘叶三萜进行提取,采用单因素试验和响应面法优化超声辅助提取工艺,通过香草醛-高氯酸法对三萜含量进行测定,并探讨其抗氧化能力.结果表明,各因素对三萜含量的影响大小依次为超声功率>液料比>超声时间,三萜最佳提取工艺为提取溶剂乙酸乙酯,液料比50:1(mL/g)、超声功率294 W,超声时间90 min.在最优的提取条件下,桃金娘叶三萜的平均含量为15.72 mg/g,优于传统浸提法和水浴提取法.在桃金娘叶三萜浓度为12.27μg/mL时,对DPPH自由基清除率可达92.52％,具有较强的抗氧化性.

2018年5月,广西某县某猪场突然有70多头35日龄猪发病,病猪症状为消瘦、腹泻、气喘、腹式呼吸,其中20多头猪病死.病死猪剖检病变为淋巴结肿大、出血;肺充血、出血,与胸膜粘连;肝充血;脾有梗死点;肾充血,表面有散在的出血点;膀胱黏膜有散在的出血点.应用抗生素对病猪进行治疗无效.该猪场将其中5头病死猪每头分别采取淋巴结、肺、肝、脾、肾等病料送实验室检测.

Farmers involved in the lucrative pork trading business between China and Southeast Asian countries should be aware of a recently discovered novel porcine deltacoronavirus (PDCoV) in Guangxi province, China. A PDCoV strain, CHN/GX/1468B/2017, was isolated from the small intestinal contents of piglets with diarrhea from this region, with a titer of 1 × 10 8.0 TCID 50 /mL on LLC-PK cells. The full-length genome sequence consists of 25,399 nt as determined by next-generation sequencing and this was deposited in the GenBank (accession number MN025260.1 ). Genomic analysis showed that CHN/GX/1468B/2017 strain had 96.9~99.4% nucleotide homology with other 87 referenced PDCoV strains from different areas, and contained 6 and 9-nt deletions at positions 1,733~1,738 and 2,804~2,812, respectively, in the ORF1a gene. Phylogenetic analyses based on the whole gene sequence as well as S protein and ORF1a/1b protein sequences all showed that this strain was closely related to the Southeast Asia strain. When 7-day-old piglets were inoculated orally with the CHN/GX/1468B/2017 strain, they developed severe diarrhea, with a peak of fecal viral shedding at 4 days post-infection. Although no death or fever were observed, the CHN/GX/1468B/2017 strain produced a wide range of tissue tropism, with the main target being the intestine. Importantly, the VH:CD ratios of the jejunum and ileum in infected piglets were significantly lower than controls. These results indicate that CHN/GX/1468B/2017, isolated in China, is a novel PDCoV Southeast Asia-like strain with distinct genetic characteristics and pathogenicity. This finding enriches the international information on the genetic diversity of PDCoV.